フルオレセインイソチオシアネート FITC
WordNet
- a yellow dye that is visible even when highly diluted; used as an absorption indicator when silver nitrate solution is added to sodium chloride in order to precipitate silver chloride (turns pink when no chloride ions are left in solution and negative fluorescein ions are then absorbed) (同)fluoresceine, fluorescent_dye, resorcinolphthalein
- a family of compounds derived from horseradish and radishes and onions and mustards; source of the hotness of those plants and preparations
- exhibit or undergo fluorescence
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- けい光を発する
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2015/09/14 00:28:49」(JST)
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This article needs additional citations for verification. Please help improve this article by adding citations to reliable sources. Unsourced material may be challenged and removed. (December 2009) |
Fluorescein isothiocyanate
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Names |
Other names
FITC
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Identifiers |
CAS Registry Number
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27072-45-3 Y |
ChEBI |
CHEBI:37928 Y |
ChemSpider |
2043359 Y |
InChI
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InChI=1S/C21H11NO5S/c23-12-2-5-15-18(8-12)26-19-9-13(24)3-6-16(19)21(15)17-7-11(22-10-28)1-4-14(17)20(25)27-21/h1-9,23-24H Y
Key: GTQFZXYECNSNNC-UHFFFAOYSA-N Y
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InChI=1/C21H11NO5S/c23-12-2-5-15-18(8-12)26-19-9-13(24)3-6-16(19)21(15)17-7-11(22-10-28)1-4-14(17)20(25)27-21/h1-9,23-24H
Key: GTQFZXYECNSNNC-UHFFFAOYAK
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Jmol-3D images |
Image |
MeSH |
Fluorescein-5-isothiocyanate |
PubChem |
18730 |
SMILES
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S=C=Nc5cc1c(C(=O)OC13c4ccc(O)cc4Oc2cc(O)ccc23)cc5
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Properties |
Chemical formula
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C21H11NO5S |
Molar mass |
389.382 |
Density |
1.542 g/mL |
Melting point |
359.5 °C (679.1 °F; 632.6 K) |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Y verify (what is: Y/N?) |
Infobox references |
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Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.
A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles.
FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm.[1] Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.
References
- ^ http://www.fluorophores.tugraz.at/substance/252 Fluorophores.org
UpToDate Contents
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English Journal
- GM1 controlled lateral segregation of tyrosine kinase Lck predispose T-cells to cell-derived galectin-1-induced apoptosis.
- Novák J, Kriston-Pál E, Czibula A, Deák M, Kovács L, Monostori E, Fajka-Boja R.Author information Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, H-6726 Szeged, Hungary.AbstractOne prominent immunoregulatory function of galectin-1 (Gal-1), a β-galactoside binding mammalian lectin, is induction of apoptosis in activated T-cells by a process depending on the activity of Src family tyrosine kinase, Lck. Although the requirement for Lck in Gal-1 induced T-cell death and the ability of Gal-1 to affect the membrane localization of extracellular Gal-1-binding proteins have been well documented, the consequence of the complex and related reorganization of extra- and intracellular signaling components upon Gal-1 treatment of T-cells has not yet been revealed. Therefore, we have analyzed the plasma membrane movement of Lck upon Gal-1 triggered signaling, and the significance of this event in Gal-1 induced T-cell death. Non-receptor tyrosine kinase, Lck primarily localized in the synapse of tumor cell-T-cell during 15min of the established direct cell contact. Later, after 30min, a lateral segregation of Lck from the cell synapse was observed. The migration of Lck to the opposite of the cell contact apparently depended on the expression and cell surface presentation of Gal-1 on the effector (tumor) cells and was accompanied by phosphorylation on the negative regulatory tyrosine residue, Tyr505. Receptor tyrosine phosphatase, CD45 played crucial role in this event since CD45 deficiency or inhibition of its phosphatase activity resulted in the failure of Lck membrane movement. Level of the Gal-1-binding glycolipid GM1 ganglioside also essentially regulated Lck localization. Segregation of Lck and Gal-1 induced apoptosis was diminished in T-cells with low GM1 expression compared to T-cells with high GM1. Our results show that spatial regulation of Lck by CD45 and GM1 ganglioside determines the outcome of apoptotic response to Gal-1 and this local regulation may occur only upon intimate effector (Gal-1 expressing) cell-T-cell attachment.
- Molecular immunology.Mol Immunol.2014 Feb;57(2):302-9. doi: 10.1016/j.molimm.2013.10.010. Epub 2013 Nov 12.
- One prominent immunoregulatory function of galectin-1 (Gal-1), a β-galactoside binding mammalian lectin, is induction of apoptosis in activated T-cells by a process depending on the activity of Src family tyrosine kinase, Lck. Although the requirement for Lck in Gal-1 induced T-cell death and the a
- PMID 24231767
- The role of microRNA-26b in human adipocyte differentiation and proliferation.
- Song G, Xu G, Ji C, Shi C, Shen Y, Chen L, Zhu L, Yang L, Zhao Y, Guo X.Author information Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.AbstractRecent findings indicate that microRNAs (miRNAs) are involved in the regulatory network of adipogenesis and obesity. Thus far, only a few human miRNAs are known to function as adipogenic regulators, fanning interest in studies on the functional role of miRNAs during adipogenesis in humans. In a previous study, we used a microarray to assess miRNA expression during human preadipocyte differentiation. We found that expression of the miR-26b was increased in mature adipocytes. MiR-26b is an intronic miRNA located in the intron of CTDSP1 (carboxy terminal domain, RNA polymerase II, polypeptide A, small phosphatase 1). Target prediction and Renilla luciferase analyses revealed the phosphatase and tensin homolog gene (PTEN) as a putative target gene. In this study, we found that miR-26b was gradually upregulated during adipocyte differentiation. To understand the roles of miR-26b in adipogenesis, we adopted a loss-of-function approach to silence miR-26b stably in human preadipocytes. We found that miR-26b inhibition effectively suppressed adipocyte differentiation, as evidenced by decreased lipid droplets and the ability of miR-26b to decrease mRNA levels of adipocyte-specific molecular markers and triglyceride accumulation. Furthermore, the cell growth assay revealed that miR-26b inhibition promoted proliferation. Nevertheless, it had no effect on apoptosis. Taken together, these data indicate that miR-26b may be involved in adipogenesis and could be targeted for therapeutic intervention in obesity.
- Gene.Gene.2014 Jan 10;533(2):481-7. doi: 10.1016/j.gene.2013.10.011. Epub 2013 Oct 17.
- Recent findings indicate that microRNAs (miRNAs) are involved in the regulatory network of adipogenesis and obesity. Thus far, only a few human miRNAs are known to function as adipogenic regulators, fanning interest in studies on the functional role of miRNAs during adipogenesis in humans. In a prev
- PMID 24140453
- A highly sensitive LED-induced chemiluminescence platform for aptasensing of platelet-derived growth factor.
- Zhang X, Zhang H, Xu S, Sun Y.Author information College of Material and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China. zhangxinfeng09@cdut.cn.AbstractIn this work, we demonstrated a simple and highly sensitive LED-induced chemiluminescence (LED-CL) platform for aptasensing of proteins. It was based on the fact that fluorescein isothiocyanate (FITC) strongly catalyzed the reaction between luminol and dissolved oxygen under LED irradiation. Using FITC as a new catalytic tag, a highly sensitive sandwich type LED-CL aptasensor was fabricated for quantification of the model protein (platelet-derived growth factor-BB, PDGF-BB). Under optimized conditions, the assay for PDGF-BB was linear from 0.1 nM to 100 nM, and the detection limit was as low as 50 pM, which is better than most of other aptamer-based assays without an amplification procedure. Thrombin, bovine serum albumin, hemoglobin, cytochrome C and adenosine triphosphate showed no obvious interferences for the determination of PDGF-BB. The advantages of high sensitivity and selectivity provided by this LED-CL aptasensor will facilitate its wide application in protein assays.
- The Analyst.Analyst.2014 Jan 7;139(1):133-7. doi: 10.1039/c3an01566a. Epub 2013 Nov 1.
- In this work, we demonstrated a simple and highly sensitive LED-induced chemiluminescence (LED-CL) platform for aptasensing of proteins. It was based on the fact that fluorescein isothiocyanate (FITC) strongly catalyzed the reaction between luminol and dissolved oxygen under LED irradiation. Using F
- PMID 24179991
Japanese Journal
- The in vivo Pig-a gene mutation assay is applied to study the genotoxicity of procarbazine hydrochloride in Sprague-Dawley rats
- Characterization of Ocular Iontophoretic Drug Transport of Ionic and Non-ionic Compounds in Isolated Rabbit Cornea and Conjunctiva
- Characterization of ocular iontophoretic drug transport of ionic and non-ionic compounds in isolated rabbit cornea and conjunctiva
Related Links
- [Fluorescein 5-Isothiocyanate] [3326-32-7] | 価格や在庫、物性値などの詳細情報ページです。 ... ・川口の在庫は即日,つくばの在庫は2〜3日以内の出荷となります。・詳細につきましては,お手数ですが営業部までお問い合わせください。
- Thesaurus Antonyms Related Words Synonyms Legend: Noun 1. fluorescein isothiocyanate - a fluorochrome commonly conjugated with antibodies for use in indirect immunofluorescence fluorescein isocyanate fluorochrome - any of ...
Related Pictures
★リンクテーブル★
[★]
- 英
- fluorescein isothiocyanate、FITC
- 関
- フルオレッセインイソチオシアネート、フルオレセイン-5-イソチオシアネート
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- 英
- fluorescein isothiocyanate
- 関
- フルオレセインイソチオシアネート
[★]
フルオレセインイソチオシアネート fluorescein isothiocyanate
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イソチオシアン酸、(化合物)イソチオシアン酸塩、イソチオシアネート