フルオレサミン、フルオレスカミン
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2013/08/12 16:44:45」(JST)
[Wiki en表示]
Fluorescamine[1] |
|
IUPAC name
4'-phenylspiro[2-benzofuran-3,2'-furan]-1,3'-dione
|
|
Identifiers |
CAS number |
38183-12-9 Y |
PubChem |
37927 |
Jmol-3D images |
Image 1 |
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C1=CC=C(C=C1)C2=COC3(C2=O)C4=CC=CC=C4C(=O)O3
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Properties |
Molecular formula |
C17H10O4 |
Molar mass |
278.26 g/mol |
Melting point |
153–157 °C
|
Y (verify) (what is: Y/N?)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
Infobox references |
Fluorescamine is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products. It hence has been used as a reagent for the detection of amines and peptides. 1-100 µg of protein and down 10pg protein can be detected.[2][3] This method is found to suffer of high blanks resulting of high rate of hydrolysis due to used excess concentration. Alternative methods are based on ortho-Phthalaldehyde (OPA), Ellman's reagent (DTNB) and epicocconone.
References[edit source | edit]
- ^ Fluram at Sigma-Aldrich
- ^ Böhlen P., S. Stein,W. Dairman, and S. Undenfriend, Arch. Biochem. Biophys. 155, 1973, 213–220
- ^ protocol by Fluoprobes
English Journal
- pH-Responsive polymeric nanocarriers for efficient killing of cariogenic bacteria in biofilms.
- Zhao Z, Ding C, Wang Y, Tan H, Li J.
- Biomaterials science. 2019 Mar;7(4)1643-1651.
- Traditional antibacterial treatments, such as chlorhexidine (CHX), destroy cariogenic biofilms. However, they exert negative effects in clinical applications, for example, teeth staining, taste disturbance and harm to oral tissue after a long-term exposure. Therefore, biocompatible strategies for ef
- PMID 30723851
- Hydrophilic-interaction planar chromatography in ultra-sensitive determination of α-aminocephalosporin antibiotics. Application to analysis of cefalexin in goat milk samples using modified QuEChERS extraction technique.
- Rageh AH, Abdel-Rahim SA, Askal HF, Saleh GA.
- Journal of pharmaceutical and biomedical analysis. 2019 Mar;166()421-434.
- A highly sensitive, selective and precise HPTLC method coupled with fluorescence detection was developed and validated for the determination of α-aminocephalosporin antibiotics; namely cefalexin, cefadroxil and cefradine in their standard solutions. The applicability of the developed methodology wa
- PMID 30716654
- Biocompatibility of Amine-Functionalized Silica Nanoparticles: The Role of Surface Coverage.
- Hsiao IL, Fritsch-Decker S, Leidner A, Al-Rawi M, Hug V, Diabaté S, Grage SL, Meffert M, Stoeger T, Gerthsen D, Ulrich AS, Niemeyer CM, Weiss C.
- Small (Weinheim an der Bergstrasse, Germany). 2019 Mar;15(10)e1805400.
- Here, amorphous silica nanoparticles (NPs), one of the most abundant nanomaterials, are used as an example to illustrate the utmost importance of surface coverage by functional groups which critically determines biocompatibility. Silica NPs are functionalized with increasing amounts of amino groups,
- PMID 30721573
Japanese Journal
- A simple high-throughput method for determination of antiepileptic analogues of γ-aminobutiric acid in pharmaceutical dosage forms using microplate fluorescence reader
- Martinc Bostjan,Vovk Tomaz
- Chemical and Pharmaceutical Bulletin, 1009-1014, 2013
- … We aimed to develop a high- throughput method using a microplate reader with fluorescence detection and simple derivatization with fluorescamine. …
- NAID 130003360905
- フルオレスカミン誘導体化HPLC法による魚および水産加工品中のヒスタミン分析の性能評価
- 菊地 博之,堤 智昭,松田 りえ子
- 食品衛生学雑誌 53(2), 121-127, 2012
- わが国では,ヒスタミンを原因とする食中毒事例が毎年報告されているが,食品に含まれるヒスタミンの規格基準および公定分析法は示されていない.そこで,食品中のヒスタミンの規格試験法を開発することを目的として,既報のタンデム固相抽出を用いたヒスタミン分析法を一部改良するとともに,改良した分析法の性能を評価した.本法の妥当性を確認するために,25,50 μg/gの濃度になるようにヒスタミンを添加したマグロ試 …
- NAID 130002109175
- Derivatization of a Protein with Fluorescamine Utilizing the Tube Radial Distribution Phenomenon of Ternary Mixed Carrier Solvents in a Capillary Tube
- MASUHARA Yuji,JINNO Naoya,HASHIMOTO Masahiko,TSUKAGOSHI Kazuhiko
- Chemistry letters 40(8), 804-805, 2011-08-05
- NAID 10029281461
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- Packaging 1 g in glass bottle 100, 250 mg in glass bottle Application Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent ...
- [Fluorescamine] [38183-12-9] | 価格や在庫、物性値などの詳細情報ページです。 ... ・川口,尼崎の在庫は即日,保管在庫は2〜3営業日以内の出荷となります。 川口,尼崎の配送対象エリアは各々異なります。
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