English Journal

Effects of psoralen on chondrocyte degeneration in lumbar intervertebral disc of rats.

Yang L1, Sun X1, Geng X1.
Pakistan journal of pharmaceutical sciences.Pak J Pharm Sci.2015 Mar;28(2 Suppl):667-70.
Discuss the internal mechanism of delaying degeneration of lumber intervertebral disc. The cartilage of lumbar intervertebral disc of SD rats was selected in vitro, then cultured by tissue explant method, and identified by HE staining, toluidine blue staining and immunofluorescence. The optimal conc
PMID 25796142

miR-CLIP capture of a miRNA targetome uncovers a lincRNA H19-miR-106a interaction.

Imig J1, Brunschweiger A1, Brümmer A2, Guennewig B1, Mittal N2, Kishore S2, Tsikrika P1, Gerber AP3, Zavolan M2, Hall J1.
Nature chemical biology.Nat Chem Biol.2015 Feb;11(2):107-14. doi: 10.1038/nchembio.1713. Epub 2014 Dec 22.
Identifying the interaction partners of noncoding RNAs is essential for elucidating their functions. We have developed an approach, termed microRNA crosslinking and immunoprecipitation (miR-CLIP), using pre-miRNAs modified with psoralen and biotin to capture their targets in cells. Photo-crosslinkin
PMID 25531890

Chromatin structure analysis of single gene molecules by psoralen cross-linking and electron microscopy.

Brown CR1, Eskin JA, Hamperl S, Griesenbeck J, Jurica MS, Boeger H.
Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2015;1228:93-121. doi: 10.1007/978-1-4939-1680-1_9.
Nucleosomes occupy a central role in regulating eukaryotic gene expression by blocking access of transcription factors to their target sites on chromosomal DNA. Analysis of chromatin structure and function has mostly been performed by probing DNA accessibility with endonucleases. Such experiments av
PMID 25311125