WordNet

to an advanced stage or point; "a young man who will go very far"
at or to a certain point or degree; "I can only go so far before I have to give up"; "how far can we get with this kind of argument?"
at or to or from a great distance in space; "he traveled far"; "strayed far from home"; "sat far away from each other"
being of a considerable distance or length; "a far trek"
being the animal or vehicle on the right or being on the right side of an animal or vehicle; "the horse on the right is the far horse"; "the right side is the far side of the horse"
beyond a norm in opinion or actions; "the far right"
located at a great distance in time or space or degree; "we come from a far country"; "far corners of the earth"; "the far future"; "a far journey"; "the far side of the road"; "far from the truth"; "far in the future"
remote in time; "if we could see far into the future"; "all that happened far in the past"
to a considerable degree; very much; "a far far better thing that I do"; "felt far worse than yesterday"; "eyes far too close together"
relating to or characteristic of the western parts of the world or the West as opposed to the eastern or oriental parts; "the Western world"; "Western thought"; "Western thought"
a sandwich made from a western omelet (同)western sandwich
lying toward or situated in the west; "our companys western office"
of or characteristic of regions of the United States west of the Mississippi River; "a Western ranch"
the syllable naming the fourth (subdominant) note of the diatonic scale in solmization
a film about life in the western United States during the period of exploration and development (同)horse_opera

PrepTutorEJDIC

《距離》『遠くに』,遠くへ,はるかに / 《時間》『ずっと後まで(に),ずっと先まで(に)』 / 《程度》《形容詞・副詞[句],およびその比較級を修飾して》『ずっと』,はるかに(much),たいそう / 《おもに文・詩》(場所が)『遠くにある』,遠い / (二つのうち)『遠いほうの』,向こう側の / 長距離(長時間)にわたる / (政治的に)極端な
『西の』;西へ向かう;(風などが)西からの / 《しばしば Western》『西洋の』 / 《しばしば Western》西部の,(特に)米国西部の / 《通例 Western》(共産圏に対して)西側の / 西部劇;米国の開拓時代を描いた小説
ファ(全音階の第4音)

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/04/12 12:20:49」(JST)

wiki en

Far-western blotting is a molecular biological method which is based on the technique of western blotting to detect protein-protein interaction in vitro. While usual western blotting uses an antibody to detect a protein of interest, far-western blotting uses a non-antibody protein, which can bind the protein of interest. Thus, whereas western blotting is used for the detection of certain proteins, far-western blotting is rather employed to detect protein:protein interactions.

Method

In conventional western blotting, gel electrophoresis is used to separate proteins from a sample; these proteins are then transferred to a membrane in a 'blotting' step. In a western blot, specific proteins are then identified using an antibody probe.

Far-western blotting employs non-antibody proteins to probe the protein(s) of interest on the blot. In this way, binding partners of the probe (or the blotted) protein may be identified. The probe protein is often produced in E. coli using an expression cloning vector. Proteins in a cell lysate containing prey proteins are firstly separated by SDS or native PAGE, and transferred to a membrane, as in a standard WB. The proteins in the membrane are then denatured and renatured. The membrane is then blocked and probed, usually with purified bait protein(s). The bait proteins are detected on spots in the membrane where a prey protein is located, if the bait proteins and the prey protein together form a complex.

The probe protein can then be visualized through the usual methods— it may be radiolabelled; it may bear a specific affinity tag like His or FLAG for which antibodies exist; or there may be a protein specific antibody (to the probe protein).

Because cell extracts are usually completely denatured by boiling in detergent before gel electrophoresis, this approach is most useful for detecting interactions that do not require the native folded structure of the protein of interest.

External links

Far-Western Blotting at the US National Library of Medicine Medical Subject Headings (MeSH)
Overview at piercenet.com
Overview at utoronto.ca

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