blotting

• ブロッティング、ブロット解析、ブロット法、吸収転移法

WordNet

1. absorbent paper used to dry ink (同)blotter

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/12/01 00:53:18」(JST)

wiki en

[Wiki en表示]

A blot, in molecular biology and genetics, is a method of transferring proteins, DNA or RNA, onto a carrier (for example, a nitrocellulose PVDF or nylon membrane). In many instances, this is done after a gel electrophoresis, transferring the molecules from the gel onto the blotting membrane, and other times adding the samples directly onto the membrane. After the blotting, the transferred proteins, DNA or RNA are then visualized by colorant staining (for example, silver staining of proteins), autoradiographic visualization of radioactive labelled molecules (performed before the blot), or specific labelling of some proteins or nucleic acids. The latter is done with antibodies or hybridization probes that bind only to some molecules of the blot and have an enzyme joined to them. After proper washing, this enzymatic activity (and so, the molecules we search in the blot) is visualized by incubation with proper reactive, rendering either a colored deposit on the blot or a chemiluminiscent reaction which is registered by photographic film.

Southern blot

A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.

Southwestern blot

A Southwestern blot is based on Southern blotting and is used to identify and characterize DNA-binding proteins by their ability to bind to specific oligonucleotide probes. The proteins are separated by gel electrophoresis and are subsequently transferred to nitrocellulose membranes similar to other types of blotting.

Other blots

• Northern blot for RNA
• Reverse Northern blot for RNA
• Western blot for proteins
• Far-Western blot for Protein-Protein
• Eastern blotting for posttranslational modification
• Far-Eastern blot for Lipids, Drugs and Hormones
• Dot blot
• Slot blot

See also

• Immunoscreening

References

UpToDate Contents

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• 1. 慢性骨髄性白血病の分子遺伝学 molecular genetics of chronic myeloid leukemia
• 2. 遺伝学およびゲノミクスのためのツール：細胞遺伝学および分子遺伝学 tools for genetics and genomics cytogenetics and molecular genetics
• 3. 代謝性ミオパチーへのアプローチ approach to the metabolic myopathies
• 4. ヒトTリンパ球好性ウイルスI型：疾患関連、診断、および治療 human t lymphotropic virus type i disease associations diagnosis and treatment
• 5. 変異型クロイツフェルト・ヤコブ病 variant creutzfeldt jakob disease

English Journal

• Influence of oxidation on the susceptibility of purified desmin to degradation by μ-calpain, caspase-3 and -6.

• Chen Q1, Huang J1, Huang F2, Huang M3, Zhou G1.Author information 1Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.2Traditional Food Processing and Machinery Laboratory, Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Science, Beijing 100193, China.3Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: mhuang@njau.edu.cn.AbstractThis study was designed to investigate the effects of desmin oxidation on its degradation by proteolytic enzymes. Desmin was isolated from bovine muscle and exposed to varying oxidative conditions, and then incubated with μ-calpain, caspase-3 or -6, respectively. The extent of protein degradation was subsequently determined using SDS-PAGE and Western-blotting. Furthermore, the oxidative modification of the secondary structure of desmin was measured by circular dichroism (CD). Our results revealed that, compared with the native desmin, degradation of oxidised desmin was enhanced by caspases, but suppressed by μ-calpain. The CD spectra of desmin showed that the content of α-helix decreased from 76.2% to 52% while random coil increased from 8% to 22.4% after oxidation. These findings demonstrated that oxidative modifications of desmin changed their susceptibility to μ-calpain, caspase-3 and -6 as well as their secondary structure.
• Food chemistry.Food Chem.2014 May 1;150:220-6. doi: 10.1016/j.foodchem.2013.10.149. Epub 2013 Nov 1.
• This study was designed to investigate the effects of desmin oxidation on its degradation by proteolytic enzymes. Desmin was isolated from bovine muscle and exposed to varying oxidative conditions, and then incubated with μ-calpain, caspase-3 or -6, respectively. The extent of protein degradation w
• PMID 24360443

• Epigenetic regulation of angiotensin-converting enzyme 2 (ACE2) by SIRT1 under conditions of cell energy stress.

• Clarke NE1, Belyaev ND1, Lambert DW2, Turner AJ1.Author information 1*School of Molecular & Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, U.K.2†Integrated Biosciences, School of Clinical Dentistry, University of Sheffield, Sheffield S10 2TA, U.K.AbstractACE2 (angiotensin-converting enzyme 2) counterbalances the actions of ACE (angiotensin-converting enzyme) by metabolizing its catalytic product, the vasoactive and fibrogenic peptide AngII (angiotensin II), into Ang-(1-7) [angiotensin-(1-7)]. Enhanced ACE2 expression may be protective in diabetes, cardiovascular disease and cancer. However, relatively little is known about the specific physiological factors regulating ACE2 expression. In the present paper, we show, by Western blotting and qPCR (quantitative real-time PCR), that ACE2 expression is increased under conditions of cell stress, including hypoxic conditions, IL (interleukin)-1β treatment and treatment with the AMP mimic AICAR (5-amino-4-imidazolecarboxamide riboside). The NAD+-dependent deacetylase SIRT1 (silent information regulator T1) was found to be up-regulated after AICAR treatment but, conversely, was down-regulated after IL-1β treatment. ChIP analysis demonstrated that SIRT1 bound to the ACE2 promoter and that binding was increased after AICAR treatment, but decreased after IL-1β treatment. Inhibition of SIRT1 activity ablated the AICAR-induced increase in ACE2. In conclusion, we have established that the expression of the ACE2 transcript is controlled by the activity of SIRT1 under conditions of energy stress.
• Clinical science (London, England : 1979).Clin Sci (Lond).2014 Apr 11;126(7):507-16. doi: 10.1042/CS20130291.
• ACE2 (angiotensin-converting enzyme 2) counterbalances the actions of ACE (angiotensin-converting enzyme) by metabolizing its catalytic product, the vasoactive and fibrogenic peptide AngII (angiotensin II), into Ang-(1-7) [angiotensin-(1-7)]. Enhanced ACE2 expression may be protective in diabetes, c
• PMID 24147777

• Muscle disuse atrophy is not accompanied by changes in skeletal muscle satellite cell content.

• Snijders T1, Wall BT1, Dirks ML1, Senden JM1, Hartgens F2, Dolmans J3, Losen M4, Verdijk LB1, van Loon LJ1.Author information 1*Department of Human Movement Sciences, School for Nutrition, Toxicology and Metabolism (NUTRIM), Maastricht University, Maastricht, The Netherlands.2†Departments of Epidemiology and Surgery, School for Public Health and Primary Care (CAPHRI), Maastricht University Medical Centre+, Maastricht, The Netherlands.3‡Department of Surgery, Maastricht University Medical Centre+, Maastricht, The Netherlands.4§Department of Neuroscience, School of Mental Health and Neuroscience, Maastricht University, Maastricht, The Netherlands.AbstractMuscle disuse leads to a considerable loss in skeletal muscle mass and strength. However, the cellular mechanisms underlying disuse-induced muscle fibre atrophy remain to be elucidated. Therefore we assessed the effect of muscle disuse on the CSA (cross-sectional area), muscle fibre size, satellite cell content and associated myocellular signalling pathways of the quadriceps muscle. A total of 12 healthy young (24±1 years of age) men were subjected to 2 weeks of one-legged knee immobilization via a full-leg cast. Before and immediately after the immobilization period and after 6 weeks of natural rehabilitation, muscle strength [1RM (one-repetition maximum)], muscle CSA [single slice CT (computed tomography) scan] and muscle fibre type characteristics (muscle biopsies) were assessed. Protein and/or mRNA expression of key genes [i.e. MYOD (myogenic differentiation), MYOG (myogenin) and MSTN (myostatin)] in the satellite cell regulatory pathways were determined using Western blotting and RT-PCR (real-time PCR) analyses respectively. The present study found that quadriceps CSA declined following immobilization by 8±2% (P<0.05). In agreement, both type I and type II muscle fibre size decreased 7±3% and 13±4% respectively (P<0.05). No changes were observed in satellite cell content following immobilization in either type I or type II muscle fibres. Muscle MYOG mRNA expression doubled (P<0.05), whereas MSTN protein expression decreased 30±9% (P<0.05) following immobilization. Muscle mass and strength returned to the baseline values within 6 weeks of recovery without any specific rehabilitative programme. In conclusion, 2 weeks of muscle disuse leads to considerable loss in skeletal muscle mass and strength. The loss in muscle mass was attributed to both type I and type II muscle fibre atrophy, and was not accompanied by a decline in satellite cell content.
• Clinical science (London, England : 1979).Clin Sci (Lond).2014 Apr 1;126(8):557-66. doi: 10.1042/CS20130295.
• Muscle disuse leads to a considerable loss in skeletal muscle mass and strength. However, the cellular mechanisms underlying disuse-induced muscle fibre atrophy remain to be elucidated. Therefore we assessed the effect of muscle disuse on the CSA (cross-sectional area), muscle fibre size, satellite
• PMID 24215591

Japanese Journal

• Differential expression of heat-shock proteins in F2 offspring from F1 hybrids produced between thermally selected and normal rainbow trout strains

• Ojima Nobuhiko,Mekuchi Miyuki,Ineno Toshinao [他]
• Fisheries science 78(5), 1051-1057, 2012-09
• NAID 40019415644

• 神経膠腫におけるc-myc,CD133,MGMTの発現と予後との関連についての検討

• 角田 翔,中村 翔,櫻田 香,松田 憲一朗,佐藤 慎哉,嘉山 孝正
• 山形大学紀要. 医学 : 山形医学 30(2), 41-49, 2012-08-15
• 【背景】神経膠腫は成人脳腫瘍で最も頻度の高い疾患であるが、その治療成績はいまだ極めて不良である。近年、DNA修復酵素の1つO-6-methylguanine-DNA methyltransferase(MGMT)の遺伝子プロモーター領域のメチル化が膠芽腫(glioblastoma:GBM)の治療感受性、治療予後に相関があることが報告されている。また神経幹細胞マーカーCD133は腫瘍幹細胞マーカーと …
• NAID 110009445795

Related Links

• Blotting | Define Blotting at Dictionary.com

He is ever in his office, receiving all who come, and blotting out with his own blood the handwriting which is against them. ... a stain or reproach (Job 31:7; Prov. 9:7). To blot out sin is to forgive it (Ps. 51:1, 9; Isa. 44:22; Acts 3:19).

• Blotting - definition of blotting by The Free Dictionary

Of all who hail thy presence as the morning -- Of all to whom thine absence is the night -- The blotting utterly from out high heaven The sacred sun -- of all who, weeping, bless thee Hourly for hope- for life -- ah

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★リンクテーブル★

リンク元	「ブロット法」「吸収転移法」「ブロット解析」「ブロッティング」
拡張検索	「Western blotting」「dot blotting」「Southwestern blotting」「western blotting」

「ブロット法」

　　[★]

英

blotting

関

ブロッティング、吸収転移法、ブロット解析

「吸収転移法」

　　[★]

英

blotting

関

ブロッティング、ブロット法、ブロット解析

「ブロット解析」

　　[★]

英

blotting

関

ブロッティング、ブロット法、吸収転移法

「ブロッティング」

　　[★]

英

blotting

関

ブロット法、吸収転移法、ブロット解析

「Western blotting」

　　[★]

ウエスタンブロット法、ウエスタンブロッティング、ウエスタンブロッティング法、Westernブロット法

関

Western blot、Western immunoblot

「dot blotting」

　　[★]

• ドットブロット、ドットブロット法、ドットブロッティング

関

dot blot、dot blot method

「Southwestern blotting」

　　[★]

• サウスウェスタンブロット法、Southwesternブロット法

「western blotting」

　　[★] ウェスタンブロット法