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1. 遺伝学およびゲノミクスのためのツール：遺伝子発現プロファイリングtools for genetics and genomics gene expression profiling [show details]
…After isolating RNA from a sample, complementary DNAs (cDNAs) are synthesized by reverse transcription with an RNA-dependent DNA polymerase. This cDNA mixture is then combined with a DNA-dependent DNA polymerase …
2. アンドロゲン不応症の発症機序と臨床的特徴pathogenesis and clinical features of disorders of androgen action [show details]
…on chromosome Xq11-12. X-linkage of the AR gene was established when the human cDNA was cloned from an X-chromosome library . The AR is a member of the steroid-thyroid-retinoid superfamily of nuclear receptors…
3. 環境中のアレルゲンサンプリングallergen sampling in the environment [show details]
…for novel allergens within a known allergenic substance involves creating a complementary DNA (cDNA) library from the substance, which is then screened with serum IgE antibodies from a population of people…
4. 学会が公開する診療ガイドラインのリンク：麻疹society guideline links measles [show details]
…Health England (PHE): National measles guidelines (2017) Communicable Diseases Network Australia (CDNA): National guidelines for public health units – Measles (2019) Australian Technical Advisory Group…
5. 学会が公開する診療ガイドラインのリンク：狂犬病society guideline links rabies [show details]
…Immunisation Handbook – Rabies and other lyssaviruses (2018) Communicable Diseases Network Australia (CDNA): National guidelines for public health units on rabies virus and other lyssavirus (including Australian…

English Journal

Normalization of full-length enriched cDNA.

Bogdanova EA, Shagin DA, Lukyanov SA.
Molecular bioSystems. 2008 Mar;4(3)205-12.
Analysis of rare messages in cDNA libraries is extremely difficult due to the substantial variations in the abundance of different transcripts in cells and tissues. Therefore, for rare transcript searches and analyses, the generation of equalized (normalized) cDNA is essential. Several cDNA normaliz
PMID 18437263

A method for the construction of equalized directional cDNA libraries from hydrolyzed total RNA.

Davis C, Barvish Z, Gitelman I.
BMC genomics. 2007 Oct;8()363.
The transcribed sequences of a cell, the transcriptome, represent the trans-acting fraction of the genetic information, yet eukaryotic cDNA libraries are typically made from only the poly-adenylated fraction. The non-coding or translated but non-polyadenylated RNAs are therefore not represented. The
PMID 17925018

Construction of equalized short hairpin RNA library from human brain cDNA.

Xu L, Li J, Liu L, Lu L, Gao J, Li X.
Journal of biotechnology. 2007 Feb;128(3)477-85.
Short hairpin RNA (shRNA) library is a powerful new tool for high-throughput loss-of-function genetic screens in mammalian cells. An shRNA library can be constructed from synthetic oligonucleotides or enzymatically cleaved natural cDNA. Here, we describe a new method for constructing equalized shRNA
PMID 17187888

Japanese Journal

Systematic Isolation of Highly Transcribed Genes in Inflorescence Apices in Arabidopsis thaliana from an Equalized cDNA Library

Hyodo Hideki,Takemura Miho,Yokota Akiho [他],OHYAMA Kanji,KOHCHI Takayuki
Bioscience, biotechnology, and biochemistry 64(7), 1538-1541, 2000-07-23
… To analyze plant meristem functions, we systematically isolated thirteen cDNA clones for transcripts that are highly expressed in the inflorescence apices of <i>Arabidopsis</i> … by differential screening, using an equalized cDNA library with the clones arrayed on membranes. …
NAID 110002680127