UpToDate Contents

全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.

1. アナフィラキシーの臨床診断を裏付ける臨床検査 laboratory tests to support the clinical diagnosis of anaphylaxis
2. 胃酸分泌の生理学 physiology of gastric acid secretion
3. 多発血管炎性肉芽腫症を伴う肉芽腫症および関連血管炎の病因 pathogenesis of granulomatosis with polyangiitis and related vasculitides
4. 好酸球性筋膜炎 eosinophilic fasciitis
5. ヒトの疾患における真菌（カビ）の役割 the role of fungi molds in human disease

English Journal

T cell-derived microvesicles induce mast cell production of IL-24: Relevance to inflammatory skin diseases.

Shefler I1, Pasmanik-Chor M2, Kidron D3, Mekori YA4, Hershko AY1.Author information 1Laboratory of Allergy and Clinical Immunology, Meir General Hospital, Kfar Saba, Israel; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.2Bioinformatics unit, G.S Wise Faculty of Life Science, Tel Aviv University, Tel Aviv, Israel.3Department of Pathology, Meir General Hospital, Kfar Saba, Israel; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.4Laboratory of Allergy and Clinical Immunology, Meir General Hospital, Kfar Saba, Israel; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel. Electronic address: ymekori@clalit.org.il.AbstractBACKGROUND: It has recently been shown that microvesicles derived from activated T cells can stimulate human mast cells (MCs) to degranulate and release several cytokines.
The Journal of allergy and clinical immunology.J Allergy Clin Immunol.2014 Jan;133(1):217-224.e3. doi: 10.1016/j.jaci.2013.04.035. Epub 2013 Jun 12.
BACKGROUND: It has recently been shown that microvesicles derived from activated T cells can stimulate human mast cells (MCs) to degranulate and release several cytokines.OBJECTIVE: The aim of this study was to characterize microvesicle-induced MC expression patterns. Through identification of uniqu
PMID 23768573

Pleurocidin, a novel antimicrobial peptide, induces human mast cell activation through the FPRL1 receptor.

Pundir P1, Catalli A2, Leggiadro C3, Douglas SE3, Kulka M4.Author information 11] National Research Council, Charlottetown, Prince Edward Island, Canada [2] Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada.2National Research Council, Charlottetown, Prince Edward Island, Canada.3National Research Council, Halifax, Nova Scotia, Canada.41] National Research Council, Charlottetown, Prince Edward Island, Canada [2] National Research Council, Edmonton, Alberta, Canada.AbstractPleurocidins are a novel family of α-helical cationic antimicrobial peptides (CAPs) that are structurally and functionally similar to cathelicidins, one of the major CAP families. As cathelicidins stimulate mast cell chemotaxis and mediator release, we postulated that pleurocidins similarly activate mast cells. A screen of 20 pleurocidin peptides revealed that some were capable of degranulating the human mast cell line LAD2 (Laboratory of Allergic Diseases 2). Pleurocidin NRC-04 caused LAD2 to adhere, migrate, degranulate, and release cysteinyl leukotrienes and prostaglandin D2. Moreover, pleurocidin increased intracellular Ca(2+) mobilization in mast cells and induced the production of proinflammatory chemokines such as monocyte chemotactic protein-1/C-C motif chemokine ligand 2 (CCL2) and macrophage inflammatory protein-1β/CCL4. Our evaluation of possible cellular mechanisms suggested that G proteins, phosphoinositol-3 kinase (PI3K), phospholipase C (PLC), and phosphokinase C (PKC) were involved in pleurocidin-induced mast cell activation as evidenced by the inhibitory effects of pertussis toxin (G protein inhibitor), wortmanin (PI3K inhibitor), U-73122 (PLC inhibitor), and Ro-31-8220 (PKC inhibitor), respectively. We also found that human mast cells expressed the N-formyl-peptide receptor 1 (FPRL1) receptor and FPRL1-specific inhibitor affected pleurocidin-mediated activation of mast cell. Our finding that the novel CAP pleurocidin activated human mast cell through G protein-coupled receptor signaling suggests that this peptide might have immunomodulatory functions.
Mucosal immunology.Mucosal Immunol.2014 Jan;7(1):177-87. doi: 10.1038/mi.2013.37. Epub 2013 Jul 10.
Pleurocidins are a novel family of α-helical cationic antimicrobial peptides (CAPs) that are structurally and functionally similar to cathelicidins, one of the major CAP families. As cathelicidins stimulate mast cell chemotaxis and mediator release, we postulated that pleurocidins similarly activat
PMID 23839065

Time- and Concentration-Dependent Effects of Exogenous Serotonin and Inflammatory Cytokines on Mast Cell Function.

Gruba SM, Meyer AF, Manning BM, Wang Y, Thompson JW, Dalluge JJ, Haynes CL.Author information Department of Chemistry, University of Minnesota , 207 Pleasant St. SE, Minneapolis, Minnesota 55455, United States.AbstractMast cells play a significant role in both the innate and adaptive immune response; however, the tissue-bound nature of mast cells presents an experimental roadblock to performing physiologically relevant mast cell experiments. In this work, a heterogeneous cell culture containing primary culture murine peritoneal mast cells (MPMCs) was studied to characterize the time-dependence of mast cell response to allergen stimulation and the time- and concentration-dependence of the ability of the heterogeneous MPMC culture to uptake and degranulate exogenous serotonin using high performance liquid chromatography (HPLC) coupled to an electrochemical detector. Additionally, because mast cells play a central role in asthma, MPMCs were exposed to CXCL10 and CCL5, two important asthma-related inflammatory cytokines that have recently been shown to induce mast cell degranulation. MPMC response to both allergen exposure and cytokine exposure was evaluated for 5-HT secretion and bioactive lipid formation using ultraperformance liquid chromatography coupled to an electrospray ionization triple quadrupole mass spectrometer (UPLC-MS/MS). In this work, MPMC response was shown to be highly regulated and responsive to subtle alterations in a complex environment through time- and concentration-dependent degranulation and bioactive lipid formation. These results highlight the importance of selecting an appropriate mast cell model when studying mast cell involvement in allergic response and inflammation.
ACS chemical biology.ACS Chem Biol.2013 Dec 19. [Epub ahead of print]
Mast cells play a significant role in both the innate and adaptive immune response; however, the tissue-bound nature of mast cells presents an experimental roadblock to performing physiologically relevant mast cell experiments. In this work, a heterogeneous cell culture containing primary culture mu
PMID 24304209

Japanese Journal

Upregulation of Heme Oxygenase-1 by Degranulation in Rat Basophilic Leukemia Cells(Molecular and Cell Biology)

, , [他], , , ,
Biological & pharmaceutical bulletin 30(3), 443-446, 2007-03-01
… HO-1 mRNA transiently increased at 1-2h after RBL-2H3 cells were stimulated to degranulate. …
NAID 110006239182

204 Aspartate Protease from Alternaria Activate and Degranulate Human Eosinophils through PAR-2

松脇由典,White Thomas,堀田京子,井上良成,森山寛,紀太博仁
アレルギー 54(8・9), 1057, 2005-09-30
NAID 110004045257

Respiratory syncytial virus induces inflammation in bronchial asthma: Role of eosinophils

,
Allergology International 53(4), 301-307, 2004
… this suggests that eosinophils degranulate in the respiratory tract during RSV infection. …
NAID 130004476824