English Journal
- Measurement of ATP in single oocytes: impact of maturation and cumulus cells on levels and consumption.
- Dalton CM, Szabadkai G, Carroll J.Author information Division of Biosciences, Department of Cell and Developmental Biology, UCL, London, UK.AbstractMitochondria provide the primary source of ATP in the oocyte and early embryo and mitochondrial dysfunction and deficit of mitochondria-derived ATP has been linked to suboptimal developmental competence. We have undertaken a study of ATP in the maturing mouse oocyte using a novel recombinant FRET based probe, AT1.03. We show that AT1.03 can be successfully used to monitor cytosolic ATP levels in single live oocytes over extended time periods. We find that ATP levels undergo dynamic changes associated with specific maturational events and that oocytes display altered rates of ATP consumption at different stages of maturation. Cumulus enclosed oocytes have a higher ATP level during maturation than denuded oocytes and this can be abolished by inhibition of gap junctional communication between the oocyte and cumulus cells. Our work uses a new approach to shed light on regulation of ATP levels and ATP consumption during oocyte maturation.
- Journal of cellular physiology.J Cell Physiol.2014 Mar;229(3):353-61. doi: 10.1002/jcp.24457.
- Mitochondria provide the primary source of ATP in the oocyte and early embryo and mitochondrial dysfunction and deficit of mitochondria-derived ATP has been linked to suboptimal developmental competence. We have undertaken a study of ATP in the maturing mouse oocyte using a novel recombinant FRET ba
- PMID 24002908
- Comparison of porcine endogenous retroviruses infectious potential in supernatants of producer cells and in cocultures.
- Rodrigues Costa M, Fischer N, Gulich B, Tönjes RR.Author information Division of Medical Biotechnology, Paul-Ehrlich-Institut, Langen, Germany.AbstractBACKGROUND: Porcine endogenous retroviruses (PERV) pose a zoonotic risk potential in pig-to-human xenotransplantation given that PERV capacity to infect different human cell lines in vitro has been clearly shown in the past. However, PERV infectious potential for human peripheral blood mononuclear cells (huPBMC) has been also demonstrated, albeit with controversial results. As productive PERV infection of huPBMC involves immune suppression that may attract opportunistic pathogens as shown for other retroviruses, it is crucial to ascertain unequivocally huPBMC susceptibility for PERV. To address this question, we first investigated in vitro infectivity of PERV for huPBMC using supernatants containing highly infectious PERV-A/C. Second, huPBMC were cocultivated with PERV-A/C producer cells to come a step closer to the in vivo situation of xenotransplantation. In addition, cocultivation of huPBMC with porcine PBMC (poPBMC) isolated from German landrace pigs was performed to distinguish PERV replication competence when they were constitutively produced by immortalized cells or by primary poPBMC.
- Xenotransplantation.Xenotransplantation.2014 Jan 21. doi: 10.1111/xen.12081. [Epub ahead of print]
- BACKGROUND: Porcine endogenous retroviruses (PERV) pose a zoonotic risk potential in pig-to-human xenotransplantation given that PERV capacity to infect different human cell lines in vitro has been clearly shown in the past. However, PERV infectious potential for human peripheral blood mononuclear c
- PMID 24447212
Japanese Journal
- Industrial Info. xCELLigence(エクセリジェンス)システムによるリアルタイム共培養実験
- 平沼 秀記,小林 五月
- 細胞 44(4), 177-181, 2012-04
- NAID 40019336425
- Development of a cocultivation microdevice for toxicokinetic study in humans
- NAKAYAMA H.,KIMURA H.,KOMORI K.,FUJII T.,SAKAI Y.
- Alternatives to animal testing and experimentation : AATEX 13, 211, 2008-12-31
- NAID 10026777638
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- Is COCULTIVATION a Scrabble word? Is it Scrabble dictionary, and What is COCULTIVATION definition, Anagrams of COCULTIVATION, Scrabble score for COCULTIVATION, images of COCULTIVATION, and words that can be ... ...
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★リンクテーブル★
| リンク元 | 「共培養」「coculture」「co-culture」「cocultivate」「共存培養」 |
「共培養」
「coculture」
- n.
- v.
- 共培養する、同時培養する
「co-culture」
「cocultivate」
- v.
- 共培養する、共存培養する