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Characterization of Tn5801.Sag, a Variant of Staphylococcus aureus Tn916 Family Transposon Tn5801 That Is Widespread in Clinical Isolates of Streptococcus agalactiae.

Mingoia M, Morici E, Tili E, Giovanetti E, Montanari MP, Varaldo PE.SourceUnit of Microbiology, Departments of Biomedical Sciences and Public Health.
Antimicrobial agents and chemotherapy.Antimicrob Agents Chemother.2013 Sep;57(9):4570-4. doi: 10.1128/AAC.00521-13. Epub 2013 Jul 1.
Tn5801, originally detected in Staphylococcus aureus Mu50, is a Tn916 family element in which a unique int gene (int5801) replaces the int and xis genes in Tn916 (int916 and xis916). Among 62 tet(M)-positive tetracycline-resistant Streptococcus agalactiae isolates, 43 harbored Tn916, whereas 19 harb
PMID 23817370

Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A.

Zhang J, Yamaguchi S, Hirakawa H, Nagamune T.SourceDepartment of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Journal of bioscience and bioengineering.J Biosci Bioeng.2013 Sep;116(3):298-301. doi: 10.1016/j.jbiosc.2013.03.006. Epub 2013 Apr 12.
Streptococcus pyogenes sortase A (SpSrtA) was transduced into mammalian cells. Then SpSrtA-mediated intracellular circularization of a model protein was confirmed in a time and dose-dependent manner by Western blotting analysis. Direct transduction of SpSrtA is expected to be a strong tool for more
PMID 23587499

High Specific and Ultrasensitive Isothermal Detection of MicroRNA by Padlock Probe-Based Exponential Rolling Circle Amplification.

Liu H, Li L, Duan L, Wang X, Xie Y, Tong L, Wang Q, Tang B.SourceCollege of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University, Jinan, 250014, China.
Analytical chemistry.Anal Chem.2013 Aug 20;85(16):7941-7. doi: 10.1021/ac401715k. Epub 2013 Jul 31.
In this paper, a padlock probe-based exponential rolling circle amplification (P-ERCA) assay is developed for highly specific and sensitive detection of microRNA (miRNA). The padlock probe is composed of a hybridization sequence to miRNA and a nicking site for nicking endonuclease. Using the miRNA a
PMID 23855808

Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

Zhang Jing,Yamaguchi Satoshi,Hirakawa Hidehiko,Nagamune Teruyuki
Journal of bioscience and bioengineering 116(3), 298-301, 2013-09
… Then SpSrtA-mediated intracellular circularization of a model protein was confirmed in a time and dose-dependent manner by Western blotting analysis. …
NAID 110009657223

DNA double strand break repair enzymes function at multiple steps in retroviral infection

Sakurai Yasuteru,Komatsu Kenshi,Agematsu Kazunaga,Matsuoka Masao
Retrovirology 6, 2009-12-15
… Background: DNA double strand break (DSB) repair enzymes are thought to be necessary for retroviral infection, especially for the post-integration repair and circularization of viral cDNA. …
NAID 120002577342

In vivo circular RNA production using a constitutive promoter for high-level expression(METHODS)

Umekage So,Kikuchi Yo
Journal of bioscience and bioengineering 108(4), 354-356, 2009-10
The permuted intron-exon (PIE) method based on group I intron self-splicing is the only methodology currently available for production of circular RNA in vivo. Here, we report improvement of the circu …
NAID 110007465833