関: BPB、bromphenol blue

WordNet

used to signify the Union forces in the American Civil War (who wore blue uniforms); "a ragged blue line"
blue color or pigment; resembling the color of the clear sky in the daytime; "he had eyes of bright blue" (同)blueness
any of numerous small butterflies of the family Lycaenidae
blue clothing; "she was wearing blue"
any organization or party whose uniforms or badges are blue; "the Union army was a vast blue"
of the color intermediate between green and violet; having a color similar to that of a clear unclouded sky; "Octobers bright blue weather"- Helen Hunt Jackson; "a blue flame"; "blue haze of tobacco smoke" (同)bluish, blueish
causing dejection; "a blue day"; "the dark days of the war"; "a week of rainy depressing weather"; "a disconsolate winter landscape"; "the first dismal dispiriting days of November"; "a dark gloomy day"; "grim rainy weather" (同)dark, dingy, disconsolate, dismal, gloomy, grim, sorry, drab, drear, dreary
suggestive of sexual impropriety; "a blue movie"; "blue jokes"; "he skips asterisks and gives you the gamy details"; "a juicy scandal"; "a naughty wink"; "naughty words"; "racy anecdotes"; "a risque story"; "spicy gossip" (同)gamy, gamey, juicy, naughty, racy, risque, spicy
morally rigorous and strict; "the puritan work ethic"; "puritanic distaste for alcohol"; "she was anything but puritanical in her behavior" (同)puritanic, puritanical
turn blue
a state of depression; "he had a bad case of the blues" (同)blue devils, megrims, vapors, vapours
a type of folksong that originated among Black Americans at the beginning of the 20th century; has a melancholy sound from repeated use of blue notes
a process that makes something blue (or bluish) (同)blueing
used to whiten laundry or hair or give it a bluish tinge (同)blueing, blue

PrepTutorEJDIC

『青い』,あい色の / 青黒い / 《話》陰気な,憂うつな / 〈U〉『青色』,あい色;青色の着物 / 〈U〉〈C〉青色絵の具,あい色染料 / 《the~》《詩》青空,青い海 / 《the blues》《話》気のふさぎ,うれいの色 / 《the blues》《ときに単数扱い》(ジャズ音楽の)ブルース / …'を'青色にする
= bluing

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2012/07/01 11:47:33」(JST)

wiki en

Bromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein) is used as an acid-base indicator, a color marker and a dye.

Acid-base indicator

Bromophenol blue (pH indicator)
below pH 3.0		above pH 4.6
3.0	↔	4.6

As an acid-base indicator its useful range lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to purple at pH 4.6; this reaction is reversible. Bromophenol blue is structurally related to phenolphthalein (a popular indicator).

Color marker

Bromophenol blue is also used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein in a gel; the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in TAE buffer or TBE buffer, bromophenol blue migrates at the same rate as a DNA fragment of approximately 500 base pairs. Xylene cyanol and Orange G may also be used for this purpose.^[1]

Dye

Bromophenol blue is also used as a dye. At neutral pH, the dye absorbs red light most strongly and transmits blue light. Solutions of the dye therefore are blue. At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution. In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red color. This phenomenon is called dichromatic color.^[2] Bromophenol blue is the substance with the highest known value of Kreft's dichromaticity index.^[3] This means that it has the largest change in color hue, when the thickness or concentration of observed sample increases or decreases.

External links

Bromophenol blue at OpenWetWare

References

^ "Agarose gel electrophoresis (basic method)". http://www.methodbook.net/dna/agarogel.html. Retrieved 2008-04-07.
^ Kreft S, Kreft M (2007). "Physicochemical and physiological basis of dichromatic colour". Naturwissenschaften 94 (11): 935–9. DOI:10.1007/s00114-007-0272-9. PMID 17534588.
^ Kreft S, Kreft M. (2009). "Quantification of dichromatism: a characteristic of color in transparent materials". Journal of the Optical Society of America A 26 (7): 1576–1581. Bibcode 2009JOSAA..26.1576K. DOI:10.1364/JOSAA.26.001576. http://www.opticsinfobase.org/josaa/abstract.cfm?URI=josaa-26-7-1576.

UpToDate Contents

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English Journal

Non-invasive high throughput approach for protein hydrophobicity determination based on surface tension.

Amrhein S1, Bauer KC1, Galm L1, Hubbuch J2.
Biotechnology and bioengineering.Biotechnol Bioeng.2015 Dec;112(12):2485-94. doi: 10.1002/bit.25677. Epub 2015 Oct 18.
The surface hydrophobicity of a protein is an important factor for its interactions in solution and thus the outcome of its production process. Yet most of the methods are not able to evaluate the influence of these hydrophobic interactions under natural conditions. In the present work we have estab
PMID 26074500

Antimutagenicity and induction of antioxidant defense by flavonoid rich extract of Myrcia bella Cambess. in normal and tumor gastric cells.

Serpeloni JM1, Leal Specian AF2, Ribeiro DL2, Tuttis K2, Vilegas W3, Martínez-López W4, Dokkedal AL5, Saldanha LL6, de Syllos Cólus IM2, Varanda EA7.
Journal of ethnopharmacology.J Ethnopharmacol.2015 Nov 5. pii: S0378-8741(15)30203-8. doi: 10.1016/j.jep.2015.11.003. [Epub ahead of print]
ETHNOPHARMACOLOGICAL RELEVANCE: The Brazilian "Cerrado" is an important source of natural products, such as Myrcia bella Cambess (MB, also known as "mercurinho"). MB leaves are popularly used for the treatment of diabetes and gastrointestinal disorders; however, only its hypoglycemic activity has be
PMID 26549270

A color test for the convenient identification of an ingested surface activating agent.

Kinoshita H, Tanaka N, Jamal M, Takakura A, Kumihashi M, Tobiume T, Tsutsui K, Ameno K.
Soudni lekarstvi / casopis Sekce soudniho lekarstvi Cs. lekarske spolecnosti J. Ev. Purkyne.Soud Lek.2015 Fall;60(3):40-42.
Color tests are easy, simple and inexpensive methods for the qualitative identification of chemicals. A color test was applied to the stomach contents of a forensic autopsy case. The result of the test, using bromophenol blue reagent, indicated the ingestion of a commercial cleaning product containi
PMID 26419519

Japanese Journal

簡易電気泳動装置を用いたタンパク質分解酵素の働きを観察する実験

森富子
理科教育学研究 51(3), 239-244, 2011-03-10
サブマリン型簡易電気泳動装置を用いて、タンパク質の分解酵素・ペプシンの働きを短時間のうちに、客観的に判定できる実験を開発した。消化される基質タンパク質として、ウシ血清アルブミン(BSA)を用い、色素・ブロモフェノールブルー(BPB)を加えることによって、泳動後の染色、脱染の時間を省き、実験時間を短縮することができた。また、電気泳動装置を使うことにより、マイクロスケールの試薬量で、消化酵素の働きを様 …
NAID 110008513431

Open-cell Titration of Seawater for Alkalinity Measurements by Colorimetry Using Bromophenol Blue Combined with a Non-linear Least-squares Method

Okamura Kei,Kimoto Hideshi,Kimoto Takashi
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 26(6), 709-713, 2010-06-10
NAID 10026784219

A New Spectrophotometric Method for the Determination of Tianeptine in Tablets Using Ion-Pair Reagents

Ulu Sevgi Tatar,Aydogmus Zeynep
Chemical & pharmaceutical bulletin 56(12), 1635-1638, 2008-12-01
… The developed method involves formation of colored chloroform extractable ion-pair complexes of tianeptine with bromophenol blue (BPB), bromocresol green (BCG), bromothymol blue (BTB) and methyl orange (MO) in acidic medium. …
NAID 110007004690

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★リンクテーブル★

リンク元	「ブロムフェノールブルー」「bromphenol blue」「ブロモフェノールブルー」「BPB」
関連記事	「blue」「bluing」

「ブロムフェノールブルー」

Bromophenol blue
	IUPAC name 4,4'-(1,1-dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis(2,6-dibromophenol)
Identifiers
CAS number	115-39-9
PubChem	8272
ChemSpider	7973
ChEBI	CHEBI:59424
Jmol-3D images	Image 1
	SMILES Brc1cc(cc(Br)c1O)C3(OS(=O)(=O)c2ccccc23)c4cc(Br)c(O)c(Br)c4 ;
	InChI InChI=1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H ; Key: UDSAIICHUKSCKT-UHFFFAOYSA-N InChI=1/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H; Key: UDSAIICHUKSCKT-UHFFFAOYAE ;
Properties
Molecular formula	C19H10Br4O5S
Molar mass	669.96 g mol−1
	(verify) (what is: /?); Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
	Infobox references