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出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2013/02/06 17:15:04」(JST)
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"Canary Yellow" redirects here. For the color, see Yellow.
Auramine O |
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Solid Auramine O
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Auramine O in aqueous solution
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IUPAC name
bis[4-(dimethylamino)phenyl]methaniminium chloride
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Other names
Basic yellow 2, Pyocatanium aureum, aizen auramine, Pyoktanin Yellow, Canary Yellow, Pyoktanin, or C.I. 41000
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Identifiers |
CAS number |
2465-27-2 Y |
PubChem |
17170 |
ChemSpider |
16254 Y |
Jmol-3D images |
Image 1
Image 2 |
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[N@H]=C(c1ccc(N(C)C)cc1)c2ccc(N(C)C)cc2
Cl.[N@H]=C(c1ccc(N(C)C)cc1)c2ccc(N(C)C)cc2
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InChI=1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H Y
Key: KSCQDDRPFHTIRL-UHFFFAOYSA-N Y
InChI=1/C17H21N3/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4/h5-12,18H,1-4H3
Key: JPIYZTWMUGTEHX-UHFFFAOYAY
InChI=1/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
Key: KSCQDDRPFHTIRL-UHFFFAOYAK
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Properties |
Molecular formula |
C17H22ClN3 |
Molar mass |
303.83 g mol−1 |
Melting point |
267 °C
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Hazards |
R-phrases |
R22 R24 R40 |
S-phrases |
S36/37 S45 |
Y (verify) (what is: Y/N?)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
Infobox references |
Auramine O is a diarylmethane dye used as a fluorescent stain. In its pure form, Auramine O appears as yellow needle crystals. It is very soluble in water and soluble in ethanol.
Auramine O can be used to stain acid-fast bacteria (e.g. Mycobacterium, where it binds to the mycolic acid in its cell wall) in a way similar to Ziehl-Neelsen stain.[1] It can also be used as a fluorescent version of Schiff reagent.[2]
Auramine O can be used together with Rhodamine B as the Truant auramine-rhodamine stain for Mycobacterium tuberculosis.[3][4] It can be also used as an antiseptic agent. According to the packaging of Post-it brand notes, Canary Yellow is a trademark of 3m.
References
- ^ Kommareddi S, Abramowsky C, Swinehart G, Hrabak L (1984). "Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis". Hum Pathol 15 (11): 1085–9. doi:10.1016/S0046-8177(84)80253-1. PMID 6208117.
- ^ Khavkin T, Kudryavtseva M, Dragunskaya E, Polotsky Y, Kudryavtsev B (1980). "Fluorescent PAS-reaction study of the epithelium of normal rabbit ileum and after challenge with enterotoxigenic Escherichia coli". Gastroenterology 78 (4): 782–90. PMID 6986320.
- ^ Truant J, Brett W, Thomas W (1962). "Fluorescence microscopy of tubercle bacilli stained with auramine and rhodamine". Henry Ford Hosp Med Bull 10: 287–96. PMID 13922644.
- ^ Arrowood M, Sterling C (1989). "Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection". J Clin Microbiol 27 (7): 1490–5. PMC 267601. PMID 2475523. //www.ncbi.nlm.nih.gov/pmc/articles/PMC267601/.
External links
Stains
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Iron/Hemosiderin |
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Lipids |
- Sudan stain
- Sudan II
- Sudan III
- Sudan IV
- Oil Red O
- Sudan Black B
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Carbohydrates |
- Periodic acid-Schiff stain
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Amyloid |
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Bacteria |
- Gram staining
- Methyl violet/Gentian violet
- Safranin
- Ziehl–Neelsen stain/acid-fast
- Carbol fuchsin/Fuchsine
- Methylene blue
- Auramine-rhodamine stain
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Connective tissue |
- trichrome stain: Masson's trichrome stain/Lillie's trichrome
- Light Green SF yellowish
- Biebrich scarlet
- Phosphomolybdic acid
- Fast Green FCF
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Other |
- H&E stain
- Silver stain
- Grocott's methenamine silver stain
- Warthin–Starry stain
- Methyl blue
- Wright's stain
- Giemsa stain
- Gömöri trichrome stain
- Neutral red
- Janus Green B
- Alcian blue stain
- Movat's stain
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Tissue stainability |
- Acidophilic
- Basophilic
- Chromophobic
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UpToDate Contents
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English Journal
- Bone marrow aspirate microscopy v. bone marrow trephine biopsy microscopy for detection of Mycobacterium tuberculosis infection.
- Sedick Q1, Vaughan J, Tebogo P, Alli NA.
- South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde.S Afr Med J.2015 Sep 14;105(9):773-5. doi: 10.7196/SAMJnew.8171.
- BACKGROUND: Tuberculosis (TB) remains a global health problem. According to the 2013 Global Report on Tuberculosis, 8.6 million people developed TB in 2012 and 1.3 million died from the disease. An estimated 13% of people who developed TB in 2012 were HIV-positive, and 75% of these lived in Africa.
- PMID 26428979
- Simultaneous staining of sputum smears for acid-fast and lipid-containing Myobacterium tuberculosis can enhance the clinical evaluation of antituberculosis treatments.
- Kayigire XA1, Friedrich SO1, van der Merwe L2, Donald PR3, Diacon AH4.
- Tuberculosis (Edinburgh, Scotland).Tuberculosis (Edinb).2015 Aug 13. pii: S1472-9792(15)30059-7. doi: 10.1016/j.tube.2015.08.001. [Epub ahead of print]
- Dormant, slow-growing, antibiotic-tolerant Mycobacterium tuberculosis undermine the shortening of tuberculosis treatment to less than 6 months and are thought to be characterised by intracellular lipid bodies. Antibiotic effects on such persisting bacilli escape evaluation as they cannot be readily
- PMID 26318558
- Detection of Acid Fast Bacilli in Saliva using Papanicolaou Stain Induced Fluorescence Method Versus Fluorochrome Staining: An Evaluative Study.
- Munot PP1, Mhapuskar AA2, Ganvir SM3, Hazarey VK3, Mhapuskar MA4, Kulkarni D5.
- Journal of international oral health : JIOH.J Int Oral Health.2015 Jul;7(7):115-20.
- BACKGROUND: Fifty years after effective chemotherapy, tuberculosis (TB) still remains leading infectious cause of adult mortality. The aim of present study was to evaluate diagnostic utility of papanicolaou (Pap) stain induced fluorescence microscopic examination of salivary smears in the diagnosis
- PMID 26229384
Japanese Journal
- P-125 Auramine O-CTC二重染色法による呼吸活性をもつ抗酸菌の迅速検出(ポスター発表)
- Effect of Stereochemistry on the Molecular Aggregation of Phenylalanine Dipeptide-type Surfactants
- Ohta Akio,Shirai Mari,Asakawa Tsuyoshi [他],Miyagishi Shigeyoshi
- Journal of Oleo Science 57(12), 659-667, 2008
- … Furthermore, the fluorescence intensity of auramine increased strikingly in the N-dodecanoyl-L-phenylalanyl-L-phenylalanine (homo-chiral dipeptide-type surfactant) system. … The fluorescence intensity of auramine in the aggregate of homo-chiral dipeptide-type surfactant was 20 times larger than that in the hetero-chiral dipeptide-type surfactant. …
- NAID 130000435864
- 化学物質に対する活性汚泥の酸素吸収速度と生物吸着の関連性
- 三原 祐一,井上 達明,横田 勝司
- 藥學雜誌 125(2), 225-229, 2005-02-01
- 近年, 科学技術の進展に伴って, 多種多様の化学物質が広く用いられ, すぐれた製品を多数生産し, 生活の向上に貢献してきた. 現在, データベースChemical abstracts serviceに登録されている化学物質の総数は2000万種を超えており, 日本ではその内の5-7万種程度が医薬品や生活関連物質の製造を目的として日常利用されている. これらの化学物質の中には環境に放出される有害な化学 …
- NAID 110003666171
Related Links
- auramine [ôr′əmēn] a yellow aniline dye used in the manufacture of paints, textiles, and rubber products. The experimental carcinogen in animals has been identified as a cause of bladder cancer in humans. Also called dimethylaniline.
- auramine stains should be kept in the dark, and this is true also for stained smears to prevent rapid fading.
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