- 関
- in situ nick-end labeling
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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2018/03/04 09:35:29」(JST)
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Mouse liver showing an apoptotic cell stained with TUNEL
Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3′- hydroxyl termini in the double-strand DNA breaks generated during apoptosis.[1][2]
Contents
- 1 Method
- 2 History
- 3 References
- 4 External links
Method
TUNEL is a method for detecting apoptotic DNA fragmentation, widely used to identify and quantify apoptotic cells, or to detect excessive DNA breakage in individual cells.[3] The assay relies on the use of terminal deoxynucleotidyl transferase (TdT), an enzyme that catalyzes attachment of deoxynucleotides, tagged with a fluorochrome or another marker, to 3'-hydroxyl termini of DNA double strand breaks. It may also label cells having DNA damaged by other means than in the course of apoptosis.
History
The fluorochrome-based TUNEL assay applicable for flow cytometry, combining the detection of DNA strand breaks with respect to the cell cycle-phase position, was originally developed by Gorczyca et al.[4] Concurrently, the avidin-peroxidase labeling assay applicable for light absorption microscope was described by Gavrieli et al.,.[5] Since 1992 the TUNEL has become one of the main methods for detecting apoptotic programmed cell death.[6] However, for years there has been a debate about its accuracy, due to problems in the original assay which caused necrotic cells to be inappropriately labeled as apoptotic.[7] The method has subsequently been improved dramatically and if performed correctly should only identify cells in the last phase of apoptosis.[8][9] New methods incorporate the dUTPs modified by fluorophores or haptens, including biotin or bromine, which can be detected directly in the case of a fluorescently-modified nucleotide (i.e., fluorescein-dUTP), or indirectly with streptavidin or antibodies, if biotin-dUTP or BrdUTP are used, respectively. The most sensitive of them is the method utlilizing incorporation of BrdUTP by TdT followed by immuocytochemical detection of BrdU.[10]
References
- ^ Gorczyca W, Traganos F, Jesionowska H, Darzynkiewicz Z. Presence of DNA strand breaks and increased sensitivity of DNA in situ to denaturation in abnormal human sperm cells. Analogy to apoptosis of somatic cells. Exp Cell Res 1993; 207:202-205. PMID 8391465 doi:10.1006/excr.1993.1182
- ^ Lozano GM, Bejarano I, Espino J, González D, Ortiz A, García JF, Rodríguez AB, Pariente JA (2009). "Density gradient capacitation is the most suitable method to improve fertilization and to reduce DNA fragmentation positive spermatozoa of infertile men". Anatolian Journal of Obstetrics & Gynecology. 3 (1): 1–7.
- ^ Lozano G.M., Bejarano, I., Espino, J., González, D., Ortiz, A., García, J.F., Rodríguez, A.B., Pariente, J.A. 2009). "Relationship between Caspase Activity and Apoptotic Markers in Human Sperm in Response to Hydrogem Peroxide and Progesterone". Journal of Reproduction and Development 55(6): 615-621.]
- ^ Gorczyca W, Bruno S, Darzynkiewicz RJ, Gong J, Darzynkiewicz Z. (1992) DNA strand breaks occurring during apoptosis: Their early in situ detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors. Intl J Onc. 1:639-648. PMID 21584593
- ^ Gavrieli Y, Sherman Y, Ben-Sasson SA (1992). "Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation". J. Cell Biol. 119:493–501. 119: 493–501. doi:10.1083/jcb.119.3.493. PMC 2289665 . PMID 1400587.
- ^ Darzynkiewicz, Z, Galkowski, D, Zhao, H. (2008) Analysis of apoptosis by cytometry using TUNEL assay. Methods, 44;250-254. PMID 18314056, doi: 10.1016/j.ymeth.2007.11.008
- ^ Grasl-Kraupp B, Ruttkay-Nedecky B, Koudelka H, Bukowska K, Bursch W, Schulte-Hermann R (1995). "In situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: a cautionary note". Hepatology. 21 (5): 1465–8. doi:10.1002/hep.1840210534. PMID 7737654.
- ^ Negoescu A, Lorimier P, Labat-Moleur F, Drouet C, Robert C, Guillermet C, Brambilla C, Brambilla E (1996). "In situ apoptotic cell labeling by the TUNEL method: improvement and evaluation on cell preparations". J Histochem Cytochem. 44 (9): 959–68. doi:10.1177/44.9.8773561. PMID 8773561.
- ^ Negoescu A, Guillermet C, Lorimier P, Brambilla E, Labat-Moleur F (1998). "Importance of DNA fragmentation in apoptosis with regard to TUNEL specificity". Biomed Pharmacother. 52 (6): 252–8. doi:10.1016/S0753-3322(98)80010-3. PMID 9755824.
- ^ Li X, Darzynkiewicz Z. (1995) Labeling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation. Cell Proliferation 28: 571-579. DOI: 10.1111/j.1365-2184.1995.tb00045.x PMID 8555370
External links
- TUNEL at the US National Library of Medicine Medical Subject Headings (MeSH)
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English Journal
- Cryosurgery and rhTNF-α play synergistic effects on a rat cortex C6 glioma model.
- Huang KM, Peng M, Feng YQ, Huang H, Tu HJ, Luo J, Zhang L, Yuan XH, Wang LC.SourceDepartment of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China.
- Cryobiology.Cryobiology.2012 Feb;64(1):43-9. Epub 2011 Sep 29.
- Glioma, a type of brain tumor originating from glioma cells, varies widely in aggressiveness and causes serious symptoms, but the treatments are limited. Studies have shown that cryosurgery has multiple effects on tumor treatments, and administration of human tumor necrosis factor-alpha (rhTNF-α) a
- PMID 21982953
- Andrographolide induces apoptosis in B16F-10 melanoma cells by inhibiting NF-κB-mediated bcl-2 activation and modulating p53-induced caspase-3 gene expression.
- Pratheeshkumar P, Sheeja K, Kuttan G.SourceAmala Cancer Research Centre , Amala Nagar, Thrissur, Kerala , India.
- Immunopharmacology and immunotoxicology.Immunopharmacol Immunotoxicol.2012 Feb;34(1):143-51. Epub 2011 Jun 20.
- Cancer is a disorder characterized by uncontrolled proliferation and reduced apoptosis. Inducing apoptosis is an efficient method of treating cancers. In this study, we investigated the effect of andrographolide on the induction of apoptosis as well as its regulatory effect on the activation of tran
- PMID 21682651
Japanese Journal
- Protection of Cultured Cortical Neurons by Luteolin against Oxidative Damage through Inhibition of Apoptosis and Induction of Heme Oxygenase-1
- Caspase-Independent Apoptosis Induced by Reperfusion Following Ischemia without Bile Duct Occlusion in Rat Liver
- 女性ホルモンとアルツハイマー型認知症 : アミロイドβ負荷による神経細胞障害に対する17β-エストラジオールの保護効果
Related Links
- アポトーシスの過程で生じる断片化DNAを，TUNEL（TdT-mediateddUTPnickendlabeling）法により検出するキットです。組織スライド中の断片化DNAをビオチン標識ヌクレオチドで標識した後，HRP標識ストレプトアビジンを反応させて染色 ...
- アポトーシス細胞の検出には、TUNEL法(TdT-mediated dUTP nick end labeling)が汎用されてい ます。本キットは、ターミナルトランスフェラーゼ(TdT)を用いてアポトーシスを起こしている細胞のDNAの 3'-OH末端をフルオレセイン- dUTPで標識 ...
Related Pictures
★リンクテーブル★
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ニック末端標識、ニック末端標識法
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- TUNEL
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TUNEL染色、タネル染色、TUNEL法
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- TdT-mediated dUTP nick-end labeling、terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling
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- 英
- TUNEL staining
- 関
- タネル染色、TUNEL染色
[★]
- 英
- TUNEL staining
- 関
- タネル染色、TUNEL法