English Journal

Bovine pancreatic trypsin inhibitor immobilized onto sepharose as a new strategy to purify a thermostable alkaline peptidase from cobia (Rachycentron canadum) processing waste.

França RC1, Assis CR1, Santos JF2, Torquato RJ3, Tanaka AS3, Hirata IY4, Assis DM4, Juliano MA4, Cavalli RO5, Carvalho LB Jr1, Bezerra RS6.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.J Chromatogr B Analyt Technol Biomed Life Sci.2016 Aug 18;1033-1034:210-217. doi: 10.1016/j.jchromb.2016.08.028. [Epub ahead of print]
A thermostable alkaline peptidase was purified from the processing waste of cobia (Rachycentron canadum) using bovine pancreatic trypsin inhibitor (BPTI) immobilized onto Sepharose. The purified enzyme had an apparent molecular mass of 24kDa by both sodium dodecyl sulfate polyacrylamide gel electrop
PMID 27567377

[Unfractionated heparin inhibits lipopolysaccharide-induced expression of chemokines in human endothelial cells through nuclear factor-ΚB signaling pathway].

Li X1, Ma Y, Chen T, Tang J, Ma X.
Zhonghua wei zhong bing ji jiu yi xue.Zhonghua Wei Zhong Bing Ji Jiu Yi Xue.2016 Feb;28(2):117-21. doi: 10.3760/cma.j.issn.2095-4352.2016.02.007.
OBJECTIVE: To determine the effect of unfractionated heparin (UFH) on lipopolysaccharide (LPS)-induced expression of chemokines and nuclear factor-ΚB (NF-ΚB) signaling pathway.METHODS: Human pulmonary microvascular endothelial cells (HPMECs) were cultured in vitro, and the cells between passages 3
PMID 26911942

Features of cell death, mitochondrial activation and caspase dependence of rabbit anti-T-lymphocyte globulin signaling in lymphoblastic Jurkat cells are distinct from classical apoptosis signaling of CD95.

Ziegler C1,2,3, Finke J2, Grüllich C1,2.
Leukemia & lymphoma.Leuk Lymphoma.2016;57(1):177-82. doi: 10.3109/10428194.2015.1044449. Epub 2015 May 25.
Rabbit anti-T-lymphocyte-globulin (ATG) is used for immunosuppression in organ and stem cell transplantation. The aim of this study was to investigate ATG-induced cell death compared to CD95-signaling of apoptosis. We measured features of cell death at the cell membrane, mitochondria, nuclei and cas
PMID 25927246