スダン染色
WordNet
- (microscopy) a dye or other coloring material that is used in microscopy to make structures visible
- color for microscopic study; "The laboratory worker dyed the specimen"
- color with a liquid dye or tint; "Stain this table a beautiful walnut color"; "people knew how to stain glass a beautiful blue in the middle ages"
- produce or leave stains; "Red wine stains the table cloth"
- a soiled or discolored appearance; "the wine left a dark stain" (同)discoloration, discolouration
- a republic in northeastern Africa on the Red Sea; achieved independence from Egypt and the United Kingdom in 1956 (同)Republic of the Sudan, Soudan
- a region of northern Africa to the south of the Sahara and Libyan deserts; extends from the Atlantic to the Red Sea (同)Soudan
- marked or dyed or discolored with foreign matter; "a badly stained tablecloth"; "tear-stained cheeks"
- having a coating of stain or varnish (同)varnished
- the act of spotting or staining something (同)spotting, maculation
- (histology) the use of a dye to color specimens for microscopic study
PrepTutorEJDIC
- (…で)…‘を'『汚す』,‘に'しみをつける《+『名』+『with』+『名』》 / 〈ガラス・木材・布など〉‘に'『着色する』 / (…で)〈人格・名声など〉‘を'汚す,‘に'傷をつける《+『名』+『with』+『名』》 / 汚れる,しみがつく / 〈C〉〈U〉(…についた)『しみ』,汚れ《+『on』+『名』》 / 〈U〉〈C〉着色剤,染料 / 〈C〉(人格・名声などに対する)汚点,傷《+『on』(『upon』)+『名』》
- アフリカ北東部の共和国;首都Khartoum / アフリカのサハラ砂ばくの南部地方名
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2016/07/31 08:57:11」(JST)
[Wiki en表示]
Sudan stains and Sudan dyes are synthetic organic compounds that are used as dyes for various plastics and are also used to stain sudanophilic biological samples, usually lipids. Sudan II, Sudan III, Sudan IV, Oil Red O, and Sudan Black B are important members of this class of compounds (see images below).
Contents
- 1 Staining
- 2 Dyeing
- 3 Examples
- 4 Safety
- 5 References
Staining
Sudan dyes have high affinity to fats, therefore they are used to demonstrate triglycerides, lipids, and lipoproteins. Alcoholic solutions of Sudan dyes are usually used, however pyridine solutions can be used in some situations as well.
Sudan stain test is often used to determine the level of fecal fat to diagnose steatorrhea. A small sample is dissolved in water or saline, glacial acetic acid is added to hydrolyze the insoluble salts of fatty acids, a few drops of alcoholic solution of Sudan III are added, the sample is spread on a microscopic slide, and heated twice to boil. Normally a stool sample should show only a few drops of red-orange stained fat under the microscope. The method is only semiquantitative but, due to its simplicity, it is used for screening.
Dyeing
Since they are characteristically "oil-and fat-soluble," Sudan dyes are also useful for dying plastics and fabrics.[1] Sudan dyes I-IV and Sudan Red G consist of arylazo-substituted naphthols. Such compounds are known to exist as a pair of tautomers:
Examples
Safety
Some spices exported from Asia have been adulterated with Sudan dyes, especially Sudan I and Sudan III, to enhance their colors. This finding has led to controversy because some Sudan dyes are carcinogenic in rats.[2]
References
- ^ Booth, Gerald (2000). Dyes, General Survey. Wiley-VCH. doi:10.1002/14356007.a09_073.
- ^ Larsen, John Chr. "Legal and illegal colors" Trends in Food Science & Technology (2008), 19(Suppl. 1), S60-S65. doi:10.1016/j.tifs.2008.07.008
Analytical reagents and tests
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Metals |
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Sugars, fats, and proteins |
Sugars & Starches |
- Benedict's reagent (reducing sugars etc)
- Aniline acetate test (pentoses)
- Starch indicator
- Molisch's test (carbs)
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Proteins & amino acids |
- Biuret test (proteins)
- Bicinchoninic acid assay (concentration)
- Bradford protein assay (concentration)
- Lowry protein assay (total protein)
- Pierce Protein Assay (total protein)
- Tollens' reagent (aldehyde & ketones)
- Van Slyke determination (amino acids)
- Sakaguchi test (arginine)
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Fats |
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Alcohols |
- Benedict's reagent (aldehyde & ketones etc)
- Denigés' reagent
- Lucas' reagent (types of alcohols)
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Drugs |
- Dille–Koppanyi reagent (barbiturates etc)
- Ehrlich's reagent (indoles, etc)
- Froehde reagent (opioids)
- Gallic acid reagent (drug precursor)
- Liebermann reagent (street drugs)
- Mandelin reagent (ketamine)
- Marquis reagent (MDMA, opiates, etc)
- Mecke reagent
- Pill testing (general)
- Simon's reagent
- Zimmermann reagent (Benzodiazepines)
- Zwikker reagent (barbiturates)
- Salicylate testing
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other |
- Dragendorff's reagent (alkaloids)
- Fenton's reagent (creates free radicals)
- Folin–Ciocalteu reagent (antioxidants)
- Murexide test (caffeine etc)
- Melzer's reagent (fungi)
- Marquis reagent (various)
- Nitrate test
- Nitrite test
- Luminol (blood)
- Pesticide detection kit
- Fecal coliform detection
- Prion detection kit
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Food portal Drink portal Category Commons
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Microbial and histological stains
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Iron/hemosiderin |
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Lipids |
- Sudan stain
- Sudan II
- Sudan III
- Sudan IV
- Oil Red O
- Sudan Black B
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Carbohydrates |
- Periodic acid-Schiff stain
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Amyloid |
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Bacteria |
- Gram staining
- Methyl violet/Gentian violet
- Safranin
- Ziehl–Neelsen stain/acid-fast
- Carbol fuchsin/Fuchsine
- Methylene blue
- Auramine-rhodamine stain
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Connective tissue |
- trichrome stain: Masson's trichrome stain/Lillie's trichrome
- Light Green SF yellowish
- Biebrich scarlet
- Phosphomolybdic acid
- Fast Green FCF
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Other |
- H&E stain
- Silver stain
- Grocott's methenamine silver stain
- Warthin–Starry stain
- Methyl blue
- Wright's stain
- Giemsa stain
- Gömöri trichrome stain
- Neutral red
- Janus Green B
- Alcian blue stain
- Movat's stain
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Tissue stainability |
- Acidophilic
- Basophilic
- Chromophobic
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UpToDate Contents
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English Journal
- Non-choroidal yellow melanoma showing positive staining with Sudan Black consistent with the presence of lipofuscin: a case report.
- Penouil MH1, Gourhant JY2, Segretin C3, Weedon D4, Rosendahl C5.
- Dermatology practical & conceptual.Dermatol Pract Concept.2014 Apr 30;4(2):45-9. doi: 10.5826/dpc.0402a09. eCollection 2014.
- A case of a predominantly yellow primary superficial spreading melanoma arising on the back of a 44-year-old woman is presented. Possible causes of the clinical and dermatoscopic yellow color are discussed. Staining with the histochemical stain, Sudan Black, revealed a differential uptake compared t
- PMID 24855574
- [Forensic medical implications of histomorphological changes in the bone and cartilage tissues under effect of radiation].
- Osipenkova-Vichtomova TK.
- Sudebno-meditsinskaia ekspertiza.Sud Med Ekspert.2013 Jul-Aug;56(4):16-21.
- The objective of the present work was to study roentgenological, microscopic, and histomorphological changes in the bone and cartilage tissues under effect of different doses of gamma-ray radiation from Gammatron-2 (GUT Co 400) and betatron bremsstrahlung radiation (25 MeV). The total radiation dose
- PMID 24428051
- Anatomy and development of the endodermis and phellem of Quercus suber L. roots.
- Machado A1, Pereira H, Teixeira RT.
- Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada.Microsc Microanal.2013 Jun;19(3):525-34. doi: 10.1017/S1431927613000287. Epub 2013 Apr 4.
- Quercus suber L. has been investigated with special attention to the stem bark and its cork formation layer, but excluding the roots. Roots are the location of infection by pathogens such as Phytophthora cinnamomi responsible for the tree's sudden death. It is widely accepted that suberin establishe
- PMID 23551860
Japanese Journal
- 悪性リンパ腫のギムザ染色における細胞質内空胞の検討
Related Links
- Qualitative fecal fat excretion or stain for fat globules (Sudan stain) can be a quick and simple way to screen for fat malabsorption. 57 If properly performed, a Sudan stain on a spot sample of stool can detect more than 90% of 58
- A sudan stain makes it possible for pathologists to spot fat in a sample. Fecal Sudan stain tests are used to check for signs of steatorrhea, or fats in the feces. This can indicate that a patient has a malabsorption problem, or a disorder of the pancreas. Testing can also be used with blood samples in patients with suspected blood disorders.
- Sudan Black B Stain : Purpose, Principle, Procedure and Interpretation November 18, 2018 Dhurba Giri CYTOPATHOLOGY , HEMATOLOGY 1 Sudan Black B (SBB) is a fat soluble dye which has very high affinity for neutral fats and lipids .
★リンクテーブル★
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- 英
- Sudan stain
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- 関
- coloring agent、dye、staining、tissue stain