・ッターエルベージェムホモジナイザー、Potter-Elvehjemホモジナイザー
WordNet
- cause to become equal or homogeneous as by mixing; "homogenize the main ingredients" (同)homogenise
- become homogeneous or similar, as by mixing; "The two liquids homogenized in the blender" (同)homogenise
- break up the fat globules of; "homogenized milk" (同)homogenise
- work lightly; "The old lady is pottering around in the garden" (同)putter
- a craftsman who shapes pottery on a potters wheel and bakes them it a kiln (同)thrower, ceramicist, ceramist
PrepTutorEJDIC
- =putter
- 陶工,陶芸家
UpToDate Contents
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English Journal
- Homogenization of mammalian tissue.
- Simpson RJ.AbstractINTRODUCTION: To purify or characterize an intracellular protein, it is important to choose an efficient method for disrupting the cell or tissue that rapidly releases the protein from its intracellular compartment into a buffer that is not harmful to the biological activity of the protein of interest. One of the most widely used methods for disrupting soft tissues is homogenization. This protocol describes three processes for homogenization of animal tissues using mechanical shear: a Potter-Elvehjem glass-Teflon homogenizer, a Dounce hand homogenizer, or a handheld Waring Blendor. These methods are rapid and pose little risk to proteins other than the release of proteases from other cellular compartments. Proteolytic degradation can be minimized by the inclusion of protease inhibitors in the homogenization buffers. An optional procedure to remove mucin from tissue homogenates is also presented.
- Cold Spring Harbor protocols.Cold Spring Harb Protoc.2010 Jul 1;2010(7):pdb.prot5455. doi: 10.1101/pdb.prot5455.
- INTRODUCTION: To purify or characterize an intracellular protein, it is important to choose an efficient method for disrupting the cell or tissue that rapidly releases the protein from its intracellular compartment into a buffer that is not harmful to the biological activity of the protein of intere
- PMID 20647359
- Comparison of the oxime-induced reactivation of erythrocyte and muscle acetylcholinesterase following inhibition by sarin or paraoxon, using a perfusion model for the real-time determination of membrane-bound acetylcholinesterase activity.
- Eckert S, Eyer P, Herkert N, Bumm R, Weber G, Thiermann H, Worek F.SourceWalther-Straub-Institute of Pharmacology and Toxicology, Ludwig-Maximilians-University of Munich, Goethestrasse 33, Munich, Germany.
- Biochemical pharmacology.Biochem Pharmacol.2008 Feb 1;75(3):698-703. Epub 2007 Sep 21.
- The purpose of these experiments was to compare oxime-induced reactivation rate constants of acetylcholinesterase from different human tissue sources inhibited by organophosphorus compounds. To this end, preliminary testing was necessary to generate a stable system both for working with erythrocytes
- PMID 17977518
Japanese Journal
- Insect Cell Extract Preparation by the Nitrogen Disruption Method for Cell-Free Translation
- Tarui Hiroshi,Murata Masahiro,Tani Ikuko,Imanishi Shigeo,Nishikawa Shigemichi,Hara Toshio
- 九州大学農学部紀要 45(1), 135-148, 2000-11
- … Furthermore, synthesized proteins were stable in this extract, but were degraded by proteinase in an extract prepared with a Potter-Elvehjem homogenizer. …
- NAID 110001797251
- Measurements of Macromolecule-Bound and Ultra-Filtrable Polyamines in Rat Liver Homogenized without Buffer
- Xu Yong Ji,Furuumi Naomi,Samejima Keijiro [他],NIITSU Masaru,SHIRAHATA Akira
- Biological & pharmaceutical bulletin 23(9), 1021-1026, 2000-09-01
- … Ultra-filtrable and macromolecule-bound polyamines in rat liver homogenates, made without buffer, were determined, using Potter-Elvehjem homogenizer and commercially available, pressure-aided ultrafiltration device with a membrane pore size that allows passage of particles of molecular weight no larger than 5000. …
- NAID 110003640116
- A Capillary Tube Method for Counting Viable Cells of Bifidobacterium bifidum Grown in a Solid Medium1
- NAKAMURA Hiroshi,SAMEJIMA Keijiro,TAMURA Zenzo
- Japanese Journal of Microbiology 18(2), 135-138, 1974
- … A piece of the solid medium (0.7 ml) was quantitatively obtained with the aid of an agar-puncher device and was homogenized in a Potter-Elvehjem homogenizer after the addition of 9.3 ml of sterile physiological saline. …
- NAID 130004237324
Related Links
- ゴム製ストッパーと金属ロッドは、本体には含まれていません。 製品詳細. logo: Potter- Elvehjem Tissue Grinders, KONTES. ホモジナイザー(Potter-Elvehjem型) ポッター エルベージェム・ティッシュグラインダー/コンテス - Potter-Elvehjem Tissue Grinders, ...
- 研究用・試薬・機器・消耗品を販売の日本ジェネティクス株式会社のチューブ ホモジナイザー-、Potter-Elvehjem Tissue Grinders, KONTES、ポッターエルベー ジェム・ティッシュグラインダー/コンテス、ホモジナイザー(Potter-Elvehjem型)の製品 ページです。
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