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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/12/06 01:55:28」(JST)

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A peripheral blood mononuclear cell (PBMC) is any peripheral blood cell having a round nucleus.^[1] These cells consist of lymphocytes (T cells, B cells, NK cells) and monocytes, whereas erythrocytes and platelets have no nuclei, and granulocytes (neutrophils, basophils, and eosinophils) have multi-lobed nuclei.

These cells can be extracted from whole blood using ficoll, a hydrophilic polysaccharide that separates layers of blood, and gradient centrifugation,^[2] which will separate the blood into a top layer of plasma, followed by a layer of PBMCs and a bottom fraction of polymorphonuclear cells (such as neutrophils and eosinophils) and erythrocytes. The polymorphonuclear cells can be further isolated by lysing the red blood cells. Basophils are sometimes found in both the denser and the PBMC fractions.^[2]

Research uses

Many scientists conducting research in the fields of immunology (including auto-immune disorders), infectious disease, hematological malignancies, vaccine development, transplant immunology, and high-throughput screening are frequent users of PBMCs. In many cases, PBMCs are derived from blood banks.

PBMCs have been thought to be an important route of vaccination. PBMCs from cancer patients can be extracted and cultured in vitro. Subsequently, PBMCs are challenged with tumor antigens such as tumor stem cell antigen. Inflammatory cytokines are usually added to aid in antigen uptake and recognition by PBMCs.

References

^ Delves, Peter, et al. Roitt's Essential Immunology, 11th Ed. ISBN 978-1-4051-3603-7
^ ^a ^b Miyahira, Andrea (22 Nov 2012). "Types of immune cells present in human PBMC". sanguinebio.com. Retrieved 23 Sep 2014.

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English Journal

Dual Positive Regulation of Embryo Implantation by Endocrine and Immune Systems - Step-by-Step Maternal Recognition of the Developing Embryo.

Fujiwara H1, Araki Y2, Imakawa K3, Saito S4, Daikoku T5, Shigeta M6, Kanzaki H7, Mori T8.
American journal of reproductive immunology (New York, N.Y. : 1989).Am J Reprod Immunol.2016 Mar;75(3):281-9. doi: 10.1111/aji.12478. Epub 2016 Jan 12.
In humans, HCG secreted from the implanting embryo stimulates progesterone production of the corpus luteum to maintain embryo implantation. Along with this endocrine system, current evidence suggests that the maternal immune system positively contributes to the embryo implantation. In mice, immune c
PMID 26755274

Clinical efficacy of a new CD28-targeting antagonist of T cell co-stimulation in a non-human primate model of collagen-induced arthritis.

Vierboom MP1, Breedveld E1, Kap YS1, Mary C2, Poirier N2, 't Hart BA1,3, Vanhove B2.
Clinical and experimental immunology.Clin Exp Immunol.2016 Mar;183(3):405-18. doi: 10.1111/cei.12739. Epub 2015 Dec 16.
T cells have a central pathogenic role in the aetiopathogenesis of rheumatoid arthritis (RA), and are therefore a favoured target of immunotherapy aiming at physical or functional elimination. Here we report an efficacy test of FR104, a new co-stimulation inhibitor directly targeting CD28 on T cells
PMID 26540618

Interaction of a dengue virus NS1-derived peptide with the inhibitory receptor KIR3DL1 on natural killer cells.

Townsley E1, O'Connor G2, Cosgrove C3, Woda M1, Co M1, Thomas SJ4, Kalayanarooj S5, Yoon IK6, Nisalak A6, Srikiatkhachorn A1, Green S1, Stephens HA7, Gostick E8, Price DA8,9, Carrington M2,3, Alter G3, McVicar DW2, Rothman AL10, Mathew A1.
Clinical and experimental immunology.Clin Exp Immunol.2016 Mar;183(3):419-30. doi: 10.1111/cei.12722. Epub 2015 Nov 24.
Killer immunoglobulin-like receptors (KIRs) interact with human leucocyte antigen (HLA) class I ligands and play a key role in the regulation and activation of NK cells. The functional importance of KIR-HLA interactions has been demonstrated for a number of chronic viral infections, but to date only
PMID 26439909

Japanese Journal

A NEW SERIES OF BISBENZIMIDAZOLE DERIVATIVES : SYNTHESIS, ANTITUMOR ACTIVITY AND LOW TOXICITY ON PBMC

Wang Xiu-Jun,Wang Qing-He,Liu Chao [他]
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NAID 40019392971

Characteristic Analysis of Peripheral Blood Mononuclear Cell Apoptosis in Coke Oven Workers

ZHANG Hong Mei,NIE Ji Sheng,LI Xin [他],NIU Qiao
Journal of occupational health 54(1), 44-50, 2012-01-01
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Ito Atsushi,Matsuo Junji,Nakamura Shinji,Yoshida Asahi,Okude Miho,Hayashi Yasuhiro,Sakai Haruna,Yoshida Mitsutaka,Takahashi Kaori,Yamaguchi Hiroyuki
PLoS ONE 7(1), e30270, 2012-01-00
… Therefore, we studied the effect of Protochlamydia on the survival of human immortal cell line, HEp-2 cells and primary peripheral blood mononuclear cells (PBMC). …
NAID 120003783337