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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/11/19 13:49:14」(JST)

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1. 成人における血栓性血小板減少性紫斑病-溶血性尿毒症症候群の原因 causes of thrombotic thrombocytopenic purpura hemolytic uremic syndrome in adults
2. 内皮：プライマー the endothelium a primer

English Journal

Cytotoxicity and DNA damage properties of tert-butylhydroquinone (TBHQ) food additive.

Eskandani M1, Hamishehkar H2, Ezzati Nazhad Dolatabadi J3.Author information 1Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.2Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.3Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: ezzatij@tbzmed.ac.ir.AbstractTert-butylhydroquinone (TBHQ) was tested for potential cytotoxicity and genotoxicity upon A549 lung cancer cells and Human Umbilical Vein Endothelial Cells (HUVEC). Cytotoxicity was evaluated by MTT assay and flow cytometry analysis, while genotoxicity was assessed in vitro by alkaline comet, DNA fragmentation and DAPI staining assays. TBHQ dose- and time-dependently decreased the growth of A549 and HUVEC cells. Flow cytometry analyses determined early and late apoptosis in the treated cells. Also, single strand DNA breaking has been observed through comet assay technique. In addition, morphology of DAPI stained cells and DNA fragmentation assay using gel electrophoresis showed clear fragmentation in the chromatin and DNA rings within the nucleus of cell's treated TBHQ.
Food chemistry.Food Chem.2014 Jun 15;153:315-20. doi: 10.1016/j.foodchem.2013.12.087. Epub 2014 Jan 3.
Tert-butylhydroquinone (TBHQ) was tested for potential cytotoxicity and genotoxicity upon A549 lung cancer cells and Human Umbilical Vein Endothelial Cells (HUVEC). Cytotoxicity was evaluated by MTT assay and flow cytometry analysis, while genotoxicity was assessed in vitro by alkaline comet, DNA fr
PMID 24491735

EGFP-EGF1-conjugated nanoparticles for targeting both neovascular and glioma cells in therapy of brain glioma.

Zhang B1, Wang H1, Liao Z2, Wang Y2, Hu Y3, Yang J3, Shen S2, Chen J2, Mei H1, Shi W1, Hu Y4, Pang Z5, Jiang X2.Author information 1Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, PR China.2School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, 826 Zhangheng Road, Shanghai 201203, PR China.3College of Pharmacy, Jiamusi University, Jiamusi 154007, PR China.4Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, PR China. Electronic address: dr_huyu@126.com.5School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, 826 Zhangheng Road, Shanghai 201203, PR China. Electronic address: zqpang@fudan.edu.cn.AbstractAs neovascular and glioma cells were closely associated and might be mutually promoted in glioma growth, a dual-targeting strategy targeting to both neovascular and glioma cells would be more promising as compared with those targeting one of them. In this study, we reported a drug delivery system where nanoparticles were decorated with EGFP-EGF1 (ENP), a fusion protein derived from factor VII with special affinity for tissue factor (TF) over-expressed in glioma tissues, to facilitate anti-glioma delivery of paclitaxel (PTX) by targeting both neovascular and glioma cells. In vitro protein binding assay demonstrated that EGFP-EGF1 bound well to C6 cells and perturbed human umbilical vein endothelial cells (HUVEC) with a concentration-dependent manner but not to unperturbed HUVEC. EGFP-EGF1-TF interaction significantly enhanced nanoparticles uptake by perturbed HUVEC and glioma C6 cells as well as nanoparticles penetration in C6 glioma spheroids, and thus improved the cytotoxicity of their payload in both monolayer cells and glioma spheroids models. In vivo imaging of glioma-bearing mice demonstrated the specific accumulation of ENP in glioma tissues. In vivo distribution of nanoparticles intuitively showed ENP mainly sited in both extravascular glioma cells and neovascular cells. Pharmacodynamic results revealed that PTX-loaded ENP (ENP-PTX) significantly prolonged the median survival time of glioma-bearing mice compared with that of any other group. TUNEL assay and H&E staining showed that ENP-PTX treatment induced significantly more cell apoptosis and tumor necrosis compared with other treatments. In conclusion, the results of this contribution demonstrated the great potential of EGFP-EGF1-functionalized nanoparticles for dual-targeting therapy of brain glioma.
Biomaterials.Biomaterials.2014 Apr;35(13):4133-45. doi: 10.1016/j.biomaterials.2014.01.071. Epub 2014 Feb 14.
As neovascular and glioma cells were closely associated and might be mutually promoted in glioma growth, a dual-targeting strategy targeting to both neovascular and glioma cells would be more promising as compared with those targeting one of them. In this study, we reported a drug delivery system wh
PMID 24529623

Priming with proangiogenic growth factors and endothelial progenitor cells improves revascularization in linear diabetic wounds.

Ackermann M1, Pabst AM1, Houdek JP1, Ziebart T2, Konerding MA1.Author information 1Institute of Functional and Clinical Anatomy, University Medical Center Mainz, D-55131 Mainz, Germany.2Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, D-55131 Mainz, Germany.AbstractIn the present study, we investigated whether proangiogenic growth factors and endothelial progenitor cells (EPCs) induce favourable effects on cutaneous incisional wound healing in diabetic mice. The proangiogenic effects of human EPCs were initially analyzed using a HUVEC in vitro angiogenesis assay and an in vivo Matrigel assay in nude mice (n=12). For the diabetic wound model, 48 Balb/c mice with streptozotocin (STZ)-induced diabetes were divided randomly into 4 groups (12 mice in each group). Subsequently, 3, 5 and 7 days before a 15‑mm full-thickness incisional skin wound was set, group 1 was pre-treated subcutaneously with a mixture of vascular endothelial growth factor (VEGF)/basic fibroblast growth factor (bFGF)/platelet-derived growth factor (PDGF) (3.5 µg of each), group 2 with 3.5 µg PDGF and group 3 with an aliquot of two million EPCs, whereas the control animals (group 4) were pre-treated with 0.2 ml saline solution. The wounds were assessed daily and the repaired tissues were harvested 7 days after complete wound closure. The angiogenesis assay demonstrated significantly increased sprout densities, areas and lengths in the EPC-treated group (all p<0.01). In the Matrigel assay, significantly increased microvessel densities, areas and sizes (all p<0.001) were also detected in the EPC-treated group. In the STZ-induced model of diabetes, the animals pre-treated with a combination of proangiogenic factors and EPCs showed in general, a more rapid wound closure. Vessel densities were >2-fold higher in the mice treated with a combination of proangiogenic factors and EPCs (p<0.05) and tensile strengths were higher in the groups treated with proangiogenic growth factors compared to the controls (p<0.05). These results suggest a beneficial effect of pre-treatment with proangiogenic growth factors and EPCs in incisional wound healing.
International journal of molecular medicine.Int J Mol Med.2014 Apr;33(4):833-9. doi: 10.3892/ijmm.2014.1630. Epub 2014 Jan 21.
In the present study, we investigated whether proangiogenic growth factors and endothelial progenitor cells (EPCs) induce favourable effects on cutaneous incisional wound healing in diabetic mice. The proangiogenic effects of human EPCs were initially analyzed using a HUVEC in vitro angiogenesis as
PMID 24452195

Japanese Journal

Depletion of Uric Acid Due to SLC22A12 (URAT1) Loss-of-Function Mutation Causes Endothelial Dysfunction in Hypouricemia

, , , , , , , , , , ,
Circulation Journal advpub(0), 2015
… mRNA of UA transporters expressed in cultured human umbilical endothelial cells (HUVEC) was detected on RT-PCR. … HUVEC do not express mRNA of URAT1, suggesting the null role of URAT1 in endothelial function.Conclusions:Depletion of UA due to SLC22A12/URAT1 loss-of-function mutations causes endothelial dysfunction in hypouricemia patients. …
NAID 130004927216

Interferon-β inhibits glioma angiogenesis through downregulation of vascular endothelial growth factor and upregulation of interferon inducible protein 10

Takano Shingo,Ishikawa Eiichi,Matsuda Masahide,Yamamoto Tetsuya,Matsumura Akira
International journal of oncology 45(5), 1837-1846, 2014-11
… The effect of IFN-β for these cell lines were evaluated by means of proliferation (MTT assay), conditioned medium induced HUVEC migration, VEGF and interferon inducible protein 10 (IP10, angiogenesis inhibitor) expression by RT-PCR and western blot analysis. … At the same dose, glioma cell-induced HUVEC migration was inhibited, but cell proliferation was not affected. …
NAID 120005496691

Coupling factor 6 の血管内皮細胞におけるケモカイン受容体発現に及ぼす影響

Suzuki Akiko,Osanai Tomohiro,Tanaka Makoto,Endo Tomohide,Murakami Kazuo,Tomita Hirofumi,Okumura Ken
弘前医学 65(2-4), 119-127, 2014-09-30
… We investigated the role of coupling factor 6 (CF6), a novel stimulator of proton importer, in the regulation of chemokine receptors in the vascular endothelial cells.Methods and Results: In microarray analysis, there were the increased expression of CC chemokine receptor 9 (CCR9) and CX3C chemokine receptor 1 (CX3CR1) and the decreased expression of CXC chemokine receptor 4 (CXCR4) in the human umbilical vascular endothelial cells (HUVEC) that were exposed to CF6. …
NAID 120005496544