cause to become equal or homogeneous as by mixing; "homogenize the main ingredients" (同)homogenise
become homogeneous or similar, as by mixing; "The two liquids homogenized in the blender" (同)homogenise
break up the fat globules of; "homogenized milk" (同)homogenise
English Journal
Homogenization of Mammalian Cells.
de Araújo ME1, Lamberti G1, Huber LA1.
Cold Spring Harbor protocols.Cold Spring Harb Protoc.2015 Nov 2;2015(11):1009-12. doi: 10.1101/pdb.prot083436.
Homogenization is the name given to the methodological steps necessary for releasing organelles and other cellular constituents as a free suspension of intact individual components. Most homogenization procedures used for mammalian cells (e.g., cavitation pump and Dounce homogenizer) rely on mechani
Cold Spring Harbor protocols.Cold Spring Harb Protoc.2014 Oct 1;2014(10):pdb.prot080010. doi: 10.1101/pdb.prot080010.
Rat or mouse liver is the most frequently used tissue for mitochondrial preparations because it is readily available, easy to homogenize, and replete with mitochondria. A motor-driven Teflon and glass Potter-Elvehjem homogenizer is the best choice for homogenizing liver, but if one is not available,
Isolation of mitochondria from tissue culture cells.
Clayton DA1, Shadel GS2.
Cold Spring Harbor protocols.Cold Spring Harb Protoc.2014 Oct 1;2014(10):pdb.prot080002. doi: 10.1101/pdb.prot080002.
The number of mitochondria per cell varies substantially from cell line to cell line. For example, human HeLa cells contain at least twice as many mitochondria as smaller mouse L cells. This protocol starts with a washed cell pellet of 1-2 mL derived from ∼10⁹ cells grown in culture. The cells a
Active Motif's dounce homogenizer enables more complete cell lysis for better cell lysate or chromatin preparations. ... Whether you are analyzing cell lysates by Western blot, or preparing chromatin for a chromatin ...
Author Archives: admindouce DOUNCE HOMOGENIZER-Solution To Our Problems Posted on March 13, 2013 by admindouce Homogenization is the disruption of samples done through various methods to achieve the desired results.