2-oxobutyrate

English Journal

Two novel classes of fused azaisocytosine-containing congeners as promising drug candidates: Design, synthesis as well as in vitro, ex vivo and in silico studies.

Sztanke M, Rzymowska J, Janicka M, Sztanke K.
Bioorganic chemistry. 2020 Jan;95()103480.
Searching for new less toxic anticancer drug candidates is a big challenge from a medical point of view. The present investigation was aimed at describing two independent synthetic approaches based on isosteric replacements, spectroscopic characteristics, in vitro anticancer and ex vivo antihaemolyt
PMID 31864156

Engineering D-Lactate Dehydrogenase from Pediococcus acidilactici for Improved Activity on 2-Hydroxy Acids with Bulky C Functional Group.

Lee HS, Park J, Yoo YJ, Yeon YJ.
Applied biochemistry and biotechnology. 2019 Dec;189(4)1141-1155.
Engineering D-lactic acid dehydrogenases for higher activity on various 2-oxo acids is important for the synthesis of 2-hydroxy acids that can be utilized in a wide range of industrial fields including the production of biopolymers, pharmaceuticals, and cosmetic compounds. Although there are many D-
PMID 31190286

The 138 residue of acetohydroxyacid synthase in Corynebacterium glutamicum is important for the substrate binding specificity.

Liu Y, Wang X, Zhan J, Hu J.
Enzyme and microbial technology. 2019 Oct;129()109357.
Corynebacterium glutamicum acetohydroxyacid synthase (AHAS), composed of two subunits IlvB and IlvN, catalyzes the first reaction in the biosynthetic pathway of branched-chain amino acids. It either condenses two pyruvates to yield acetolactate, leading to the biosynthesis of L-valine and L-leucine,
PMID 31307581

荒川賢治,曹志生,鈴木敏弘,近藤寿志,殿川亜未,小中勇二,板倉康浩,鈴木夏実,木梨陽康
天然有機化合物討論会講演要旨集 53(0), 475-480, 2011
… Lankamycin (2), produced by Streptomyces rochei 7434AN4, is a 14-membered macrolide antibiotic attached with two deoxysugars, L-arcanose and D-chalcose. … Compound 2 contains a 3-hydroxy-2-butyl side chain at C-13. … The biosynthetic origin of 1-carboxyethyl group was confirmed by incorporation of deuterium in [3-2H]3-methyl-2-oxobutyrate (10) into the C-14 position. …
NAID 110009986581

Characterization of Acetohydroxyacid Synthase I from Escherichia coli K-12 and Identification of Its Inhibitors

Chien Pham Ngoc,Moon Ji-Young,Cho Jun-Haeng [他],LEE Soo-Jae,PARK Joon-Shik,KIM Dong-Eun,PARK Yoonkyung,YOON Moon-Young
Bioscience, biotechnology, and biochemistry 74(11), 2281-2286, 2010-11-23
… The first step in branched-chain amino acid biosynthesis is catalyzed by acetohydroxyacid synthase (EC 2.2.1.6). … This reaction involves decarboxylation of pyruvate followed by condensation with either an additional pyruvate molecule or with 2-oxobutyrate. …
NAID 10027561651

GOTO Takanobu,TAKAHASHI Tomoko,KAIMASU Takumi,TAKAGI Shusaku,MOCHIZUKI Akihiko
水産増殖 53(1), 95-96, 2005
… Liver homogenates were incubated with L homoserine at 25°C for 60 min, and 2-oxobutyrate formed by the enzymic reaction was determined and defined as the enzyme activity. … Addition of DL-propargylglycine or L-cysteine into the reaction mixtures inhibited 2-oxobutyrate productions, but the inhibition manners of cysteine were different among the examined species. …
NAID 130003865838