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1. 尿酸腎結石症uric acid nephrolithiasis [show details]
… discussed separately. The two major factors that promote uric acid precipitation are a high urine uric acid concentration and an acid urine pH, which drives the following reaction toward the right, converting …
2. 胃酸分泌の生理学physiology of gastric acid secretion [show details]
…deficient mice and HDC-knockout mice have near normal basal acid secretion, a preserved acid secretory response to cholinergic agents, an absent acid secretory response to exogenous gastrin, and hypergastrinemia …
3. 尿酸による腎疾患uric acid renal diseases [show details]
… separately. Uric acid is a weak organic acid and exists in one of two forms in biological systems, depending upon the prevailing pH : the sparingly soluble undissociated uric acid form (the most highly …
4. 妊娠中の葉酸補充folic acid supplementation in pregnancy [show details]
…consume some folic acid as folate is a natural component of a variety of foods, and grain products in many countries are fortified with folic acid. This topic will discuss the role of folic acid supplementation …
5. バルプロ酸中毒valproic acid poisoning [show details]
… Valproic acid (2-propylpentanoic acid; VPA) is a branched-chain carboxylic acid introduced as an anti-epileptic drug in 1978 in the United States. It is used to treat partial and generalized seizures …

English Journal

Nutrient supplementation strategy improves cell concentration and longevity, monoclonal antibody production and lactate metabolism of Chinese hamster ovary cells.

Pérez-Rodriguez S, Ramírez-Lira MJ, Trujillo-Roldán MA, Valdez-Cruz NA.
Bioengineered. 2020 Dec;11(1)463-471.
A careful selection of culture mediums and feeds has become necessary to maximize yields of recombinant proteins during bioprocesses of mammalian cells. Supplements contain a variety of concentrate nutrients, and their beneficial effects vary according to recombinant cell lines. In this study, the e
PMID 32223359

Rapid purification method for human SFPQ by implementing zinc-induced polymerization.

Lim YW, Lee M.
Protein expression and purification. 2020 Jul;171()105626.
Splicing factor proline- and glutamine-rich (SFPQ) is an RNA-binding protein, playing significant roles in gene regulation and subnuclear body formation. Our recent serendipitous discovery showed that SFPQ binds zinc directly and forms an infinite polymer that is induced by zinc binding to the prote
PMID 32198010

Functional Analysis of a Glutamine Biosynthesis Protein from a Psychrotrophic Bacterium, GCJ02.

Gong C, You X, Zhang S, Xue D.
Indian journal of microbiology. 2020 Jun;60(2)153-159.
A putative glutamine synthetase (GS) was detected in a psychrophilic bacterium, GCJ02. For gaining greater insight into its functioning, the gene was cloned and expressed in a heterologous host, . The monomer enzyme with a molecular weight of 53.03 kDa was expressed primarily in cytosolic compartm
PMID 32255847

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