WordNet

established or well-known or widely recognized as a model of authority or excellence; "a standard reference work"; "the classical argument between free trade and protectionism"
the value behind the money in a monetary system (同)monetary standard
a board measure = 1980 board feet
any distinctive flag (同)banner
an upright pole or beam (especially one used as a support); "distance was marked by standards every mile"; "lamps supported on standards provided illumination"
a basis for comparison; a reference point against which other things can be evaluated; "the schools comply with federal standards"; "they set the measure for all subsequent work" (同)criterion, measure, touchstone
conforming to the established language usage of educated native speakers; "standard English" (American); "received standard English is sometimes called the Kings English" (British) (同)received
regularly and widely used or sold; "a standard size"; "a stock item" (同)stock
commonly used or supplied; "standard procedure"; "standard car equipment"
conforming to or constituting a standard of measurement or value; or of the usual or regularized or accepted kind; "windows of standard width"; "standard sizes"; "the standard fixtures"; "standard brands"; "standard operating procedure"
the trace of a point whose direction of motion changes (同)curved shape
a pitch of a baseball that is thrown with spin so that its path curves as it approaches the batter (同)curve ball, breaking ball, bender
a line on a graph representing data
an inferior dog or one of mixed breed (同)mongrel, mutt
a cowardly and despicable person
having or marked by a curve or smoothly rounded bend; "the curved tusks of a walrus"; "his curved lips suggested a smile but his eyes were hard" (同)curving

PrepTutorEJDIC

〈C〉〈U〉『標準』,基準,水準,規範 / 〈C〉(度量衡の)基本単位 / 〈U〉〈C〉(貨幣制度の)本位 / 〈C〉旗,軍旗;(主義・主張などの)旗じるし / 〈C〉支桂,ランプ台,しょく台 / 『標準の』,標準的な / 定評のある,権威のある
『曲線』 / 『曲がり』,曲がったもの,湾曲部 / (野球で投球の)『カーブ』 / (…の方へ)『曲がる』,湾曲する《+『to』+『名』》 / …'を'曲げる;'を'湾曲させる
のら犬 / くだらない人間

Wikipedia preview

出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2014/02/19 01:41:21」(JST)

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An example of a standard curve showing the absorbance of different concentrations of protein (two trials for each measurement). The concentration of protein in a different sample is determined by determining where on the standard curve it should go - in this case, 30 milligrams.

A standard curve is a type of graph used as a quantitative research technique. Multiple samples with known properties are measured and graphed, which then allows the same properties to be determined for unknown samples by interpolation on the graph. The samples with known properties are the standards, and the graph is the standard curve.

Standard curves are most commonly used to determine the concentration of a substance such as protein or DNA. For example, a standard curve for protein concentration is often created using known concentrations of bovine serum albumin. The property measured may be absorbance, optical density, luminescence, fluorescence, radioactivity, or other parameters.

Example[edit]

Samples treated with the Bradford assay. The brown sample (lower absorbance) contains no protein, while the blue sample (higher absorbance) contains protein. The amount of protein in the second sample can be determined by comparison to a standard curve.

The Bradford assay is a colorimetric assay that measures protein concentration. The reagent Coomassie Brilliant Blue turns blue when it binds to arginine and aromatic amino acids present in proteins, thus increasing the absorbance of the sample. The absorbance is measured using a spectrophotometer, at the maximum absorbance frequency (A_max)of the blue dye (which is 595 nm). In this case, the greater the absorbance, the higher the protein concentration.

Data for known concentrations of protein are used to make the standard curve, plotting concentration on the X axis, and the assay measurement on the Y axis. The same assay is then performed with samples of unknown concentration. To analyze the data, one locates the measurement on the Y-axis that corresponds to the assay measurement of the unknown substance and follows a line to intersect the standard curve. The corresponding value on the X-axis is the concentration of substance in the unknown sample.

References[edit]

Bradford Assay Background

(pdf format) "Bradford Protein Assay". Bio-Rad Quick Start^tm Bradford Protein Assay Instructional Manual. Retrieved May 31, 2005.

UpToDate Contents

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English Journal

Comparative analysis of methods to determine DNA methylation levels of a tumor-related microRNA gene.

Konishi Y1, Hayashi H2, Suzuki H3, Yamamoto E3, Sugisaki H1, Higashimoto H4.
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Determination of irradiation dose and distinguishing between irradiated and non irradiated fish meat by real-time PCR.

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Detection and absolute quantitation of Tomato torrado virus (ToTV) by real time RT-PCR.

Herrera-Vásquez JA1, Rubio L2, Alfaro-Fernández A3, Debreczeni DE2, Font-San-Ambrosio I3, Falk BW4, Ferriol I5.
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PMID 25956672

Japanese Journal

偏差値メトリクスを用いた欠陥モジュール予測モデルの提案

藤野啓輔,坂口英司,Yongpisanpop Papon,伊原彰紀,松本健一
ウィンターワークショップ2015・イン・宜野湾論文集 2015, 31-32, 2015-01-15
NAID 170000092618

Determination of Miglitol by Column-Switching Ion-Pair HPLC with Tris(2,2′-bipyridine)ruthenium(II)-Electrogenerated Chemiluminescence Detection

, , ,
Chemical and Pharmaceutical Bulletin 63(6), 476-480, 2015
… We have developed a highly sensitive, simple method for the quantitative determination of miglitol in standard serum samples using column-switching ion-pair HPLC with tris(2,2′-bipyridine)ruthenium(II)-electrogenerated chemiluminescence detection. … The calibration curve for miglitol in the serum samples showed good linearity (r2=0.9997) in the range of 10–2500 ng/mL. …
NAID 130005072104

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李立岗,中村聡宏,鹿嶋俊英 [他],勅使川原正臣
日本建築学会構造系論文集 80(710), 609-617, 2015
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