English Journal

The phosphorolytic exoribonucleases PNPase and RNase PH stabilize sRNAs and facilitate regulation of their mRNA targets.

Cameron TA1, De Lay NR2.
Journal of bacteriology.J Bacteriol.2016 Oct 3. pii: JB.00624-16. [Epub ahead of print]
Gene regulation by base-pairing between small non-coding RNAs (sRNAs) and their mRNA targets is an important mechanism that allows bacteria to maintain homeostasis and respond to dynamic environments. In Gram negative bacteria, sRNA pairing and regulation is mediated by several RNA-binding proteins,
PMID 27698082

On the phosphorylase activity of GH3 enzymes: A β-N-acetylglucosaminidase from Herbaspirillum seropedicae SmR1 and a glucosidase from Saccharopolyspora erythraea.

Ducatti DR1, Carroll MA2, Jakeman DL3.
Carbohydrate research.Carbohydr Res.2016 Sep 23;435:106-112. doi: 10.1016/j.carres.2016.09.015. [Epub ahead of print]
A phosphorolytic activity has been reported for beta-N-acetylglucosaminidases from glycoside hydrolase family 3 (GH3) giving an interesting explanation for an unusual histidine as catalytic acid/base residue and suggesting that members from this family may be phosphorylases [J. Biol. Chem. 2015, 290
PMID 27744113

SAMHD1, the Aicardi-Goutières syndrome gene and retroviral restriction factor, is a phosphorolytic ribonuclease rather than a hydrolytic ribonuclease.

Ryoo J1, Hwang SY1, Choi J1, Oh C2, Ahn K3.
Biochemical and biophysical research communications.Biochem Biophys Res Commun.2016 Sep 2;477(4):977-81. doi: 10.1016/j.bbrc.2016.07.013. Epub 2016 Jul 4.
SAMHD1 plays diverse roles in innate immunity, autoimmune diseases and HIV restriction, but the mechanisms involved are still unclear. SAMHD1 has been reported to have both dNTPase and RNase activities. However, whether SAMHD1 possesses RNase activity remains highly controversial. Here, we found tha
PMID 27387229

Japanese Journal

Characterization of Two α-1,3-Glucoside Phosphorylases from Clostridium phytofermentans

Nihira Takanori,Nishimoto Mamoru,Nakai Hiroyuki,Ohtsubo Ken'ichi,Kitaoka Motomitsu
Journal of Applied Glycoscience 61(2), 59-66, 2014
… Kinetic analyses of their phosphorolytic reactions with α-1,3-oligoglucans (DP = 3 and 4) revealed that Cphy_3314 utilized a typical sequential Bi Bi mechanism, while this enzyme did not exhibit any significant phosphorolytic activity for nigerose. …
NAID 130004481039

Metabolic Mechanism of Mannan in a Ruminal Bacterium, Ruminococcus albus, Involving Two Mannoside Phosphorylases and Cellobiose 2-Epimerase : Discovery of a New Carbohydrate Phosphorylase, β-1,4-Mannooligosaccharide Phosphorylase

Kawahara Ryosuke,Saburi Wataru,Odaka Rei,Taguchi Hidenori,Ito Shigeaki,Mori Haruhide,Matsui Hirokazu
Journal of Biological Chemistry 287(50), 42389-42399, 2012-12-07
… In the phosphorolytic reactions, RaMP2 had weak activity towards β-1,4-mannobiose but efficiently degraded β-1,4-mannooligosaccharides longer than β-1,4-mannobiose. …
NAID 120005108187

仁平高則,中井博之,北岡本光,仁平高則,中井博之
新潟大学農学部研究報告 64(2), 107-113, 2012-03
… Recombinant Cphy1874 protein produced in Escherichia coli showed phosphorolytic activity towardnigerose in the presence of inorganic phosphate, resulting in release of D-glucose and β-D-glucose 1-phosphate with inversionof the anomeric configuration. … Kinetic parameters of the phosphorolytic activity on nigerose were kcat 67 s-1 and Km 1.7 mM.Additionally Cphy1874 showed the highest synthetic activity with D-glucose as the acceptor in the reverse reaction with β-D-glucose 1-phosphate as the donor. …
NAID 120005239948