English Journal

Creation of endoglucanase-secreting Streptomyces lividans for enzyme production using cellulose as the carbon source.

Noda S, Kawai Y, Miyazaki T, Tanaka T, Kondo A.SourceDepartment of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan.
Applied microbiology and biotechnology.Appl Microbiol Biotechnol.2013 Jul;97(13):5711-20. doi: 10.1007/s00253-013-4880-3. Epub 2013 Apr 21.
We screened for high-activity endoglucanase (EG) as a first step toward the creation of cellulose-assimilating Streptomyces lividans transformants. EGs derived from Thermobifida fusca YX, Tfu0901, and S. lividans, cellulase B (CelB), were successfully expressed. Genes encoding Tfu0901 or CelB were i
PMID 23604559

Production and Characterization of Ethanol- and Protease-Tolerant and Xylooligosaccharides-Producing Endoxylanase from Humicola sp. Ly01.

Zhou J, Wu Q, Zhang R, Yang Y, Tang X, Li J, Ding J, Dong Y, Huang Z.SourceEngineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming 650500, People's Republic of China, College of Life Sciences, Yunnan Normal University, Kunming 650500, People's Republic of China, Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, Yunnan, Kunming, 650500, People's Republic of China.
Journal of microbiology and biotechnology.J Microbiol Biotechnol.2013 Jun 28;23(6):794-801.
This paper reports the production and characterization of crude xylanase from the newly isolated Humicola sp. Ly01. The highest (41.8 U/ml) production of the crude xylanase was obtained under the optimized conditions (w/v): 0.5% wheat bran, 0.2% KH2PO4, and 0.5% peptone; initial pH 7.0; incubation t
PMID 23676918

Engineering the pH-optimum of activity of the GH12 family endoglucanase by site-directed mutagenesis.

Tishkov VI, Gusakov AV, Cherkashina AS, Sinitsyn AP.SourceDepartment of Chemistry, M. V. Lomonosov Moscow State University, Leninskie Gory 1/11, Moscow 119991, Russia; A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Pr. 33, Moscow 119071, Russia.
Biochimie.Biochimie.2013 Jun 14. pii: S0300-9084(13)00169-7. doi: 10.1016/j.biochi.2013.05.018. [Epub ahead of print]
Endo-1,4-β-glucanase from Penicillium verruculosum (PvEGIII) belongs to family 12 of glycoside hydrolases (GH12). Analysis of the enzyme 3D model structure showed that the amino acid residue Asp98 may directly affect the pH-profile of enzyme activity since it is located at the distance of hydrogen
PMID 23774299

Japanese Journal

Characterization of Paenibacillus curdlanolyticus Intracellular Xylanase Xyn10B Encoded by the xyn10B Gene

SUDO Maya,SAKKA Makiko,KIMURA Tetsuya,RATANAKHANOKCHAI Khanok,SAKKA Kazuo
Bioscience, biotechnology, and biochemistry 74(11), 2358-2360, 2010-11-23
… rXyn10B hydrolyzed birch-wood xylan and xylooligosaccharides to produce mainly xylobiose, suggesting that it is an endoxylanase. …
NAID 10027562047

Purification and characterization of a thermostable xylanase from the brown-rot fungus Laetiporus sulphureus(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

Lee Jae-Won,Park Jun-Yeong,Kwon Mi,Choi In-Gyu
Journal of bioscience and bioengineering 107(1), 33-37, 2009-01
… sulphureus xylanase is an endoxylanase that hydrolyzes xylotriose, xylotetraose, and xylopentaose but not xylobiose. …
NAID 110007042021