hanging drop culture

懸滴培養

WordNet

  1. utter with seeming casualness; "drop a hint"; drop names"
  2. a shape that is spherical and small; "he studied the shapes of low-viscosity drops"; "beads of sweat on his forehead" (同)bead, pearl
  3. a small indefinite quantity (especially of a liquid); "he had a drop too much to drink"; "a drop of each sample was analyzed"; "there is not a drop of pity in that man"; "years afterward, they would pay the blood-money, driblet by driblet"--Kipling (同)drib, driblet
  4. stop pursuing or acting; "drop a lawsuit"; "knock it off!" (同)knock off
  5. fall or sink into a state of exhaustion or death; "shop til you drop"
  6. give birth; used for animals; "The cow dropped her calf this morning"
  7. a sudden sharp decrease in some quantity; "a drop of 57 points on the Dow Jones index"; "there was a drop in pressure in the pulmonary artery"; "a dip in prices"; "when that became known the price of their stock went into free fall" (同)dip, fall, free_fall
  8. a central depository where things can be left or picked up
  9. the act of dropping something; "they expected the drop would be successful"
  10. a free and rapid descent by the force of gravity; "it was a miracle that he survived the drop from that height" (同)fall
  11. a predetermined hiding place for the deposit and distribution of illicit goods (such as drugs or stolen property)
  12. leave or unload; "unload the cargo"; "drop off the passengers at the hotel" (同)drop_off, set_down, put down, unload, discharge
  13. change from one level to another; "She dropped into army jargon"
  14. go down in value; "Stock prices dropped"
  15. let fall to the ground; "Dont drop the dishes"
  16. lose (a game); "The Giants dropped 11 of their first 13"
  17. omit (a letter or syllable) in speaking or writing; " New Englanders drop their post-vocalic rs"
  18. take (a drug, especially LSD), by mouth; "She dropped acid when she was a teenager"
  19. terminate an association with; "drop him from the Republican ticket"
  20. to fall vertically; "the bombs are dropping on enemy targets"
  21. grow in a special preparation; "the biologist grows microorganisms"
  22. (biology) the growing of microorganisms in a nutrient medium (such as gelatin or agar); "the culture of cells in a Petri dish"
  23. the raising of plants or animals; "the culture of oysters"
  24. a particular society at a particular time and place; "early Mayan civilization" (同)civilization, civilisation
  25. the attitudes and behavior that are characteristic of a particular social group or organization; "the developing drug culture"; "the reason that the agency is doomed to inaction has something to do with the FBI culture"
  26. the tastes in art and manners that are favored by a social group
  27. decoration that is hung (as a tapestry) on a wall or over a window; "the cold castle walls were covered with hangings" (同)wall hanging
  28. a form of capital punishment; victim is suspended by the neck from a gallows or gibbet until dead; "in those days the hanging of criminals was a public entertainment"

PrepTutorEJDIC

  1. 〈しずくが〉『したたる』,垂れる《+『down』》 / 〈物が〉『落ちる』,落下する《+『down』》 / 〈人が〉『ばったり倒れる』,崩れ落ちる / 『下落する』,減少する《+『off』(『away』)》 / 終わる,やめになる / 《副詞[句]を伴って》引き離される,落後する / (…から)手を引く,やめる《+『from』(『out of』)+『名』》 / (…に)ちょっと立ち寄る,(…を)ひょっこり訪ねる《+『in』(『by,over,round』)『at』(『on』)+『名』》 / …‘を'『したたらせる』,垂らす / (…へ)…‘を'『落とす』,落下させる《+『名』+『into』(『on』)+『名』》 / …‘を'降ろす,下げる / …‘を'減少させる / …‘を'漏らす,ふと口にする / 〈人〉に〈短い手紙・伝言〉‘を'送る / 《話》…‘を'なぐり倒す,撃ち落とす / 《話》《副詞[句]を伴って》〈人〉‘を'乗り物から降ろす / (…から)〈ある音・文字〉‘を'落とす,抜かす,省く《+『名』+『from』+『名』》 / 〈人〉‘と'関係を絶つ;〈問題など〉‘を'打ち切る / …‘を'やめさせる;(…から)…‘を'追い出す《+『名from』+『名』》 / (ばくちなどで)〈金〉‘を'なくす / 〈C〉(…の)『しずく』,したたり《+『of』+『名』》 / 〈C〉(液体・事物の)少量,微量《+『of』+『名』》 / 〈C〉あめ玉,ドロップ / 《a ~》落下,降下(fall);落下距離 / 《a ~》(…の)下落,減少《+『in』+『名』》 / 《複数形で》点滴薬 / 《話》《a ~》少量の酒 / 〈C〉《おもに米》(郵便箱などの)落とし口,差し入れ口
  2. 〈U〉『教養』;修養;教化 / 〈C〉〈U〉『文化』(主として精神面をさし,生活・習慣・物の考え方などを含む)Greek cultureギリシア文化 / 〈U〉(…の)『耕作』;『栽培』,養殖,培養《+『of』+『名』》
  3. 〈U〉〈C〉絞首刑 / 《複数形で》カーテン,幕;壁紙 / 絞首刑に値する / 掛かっている,ぶら下がった
  4. 教養のある,洗練された,上品な / 栽培(養殖)された

UpToDate Contents

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English Journal

  • Development of an in vitro culture method for stepwise differentiation of mouse embryonic stem cells and induced pluripotent stem cells into mature osteoclasts.
  • Nishikawa K, Iwamoto Y, Ishii M.Author information Department of Immunology and Cell Biology, Graduate School of Medicine/Frontier Biosciences, Osaka University, Yamada-oka 2-2, Suita, Osaka, 565-0871, Japan, nishi@ifrec.osaka-u.ac.jp.AbstractThe development of methods for differentiation of embryonic stem cells (ESCs) and induced pluripotent stem cell (iPSCs) into functional cells have helped to analyze the mechanism regulating cellular processes and to explore cell-based assays for drug discovery. Although several reports have demonstrated methods for differentiation of mouse ESCs into osteoclast-like cells, it remains unclear whether these methods are applicable for differentiation of iPSCs to osteoclasts. In this study, we developed a simple method for stepwise differentiation of mouse ESCs and iPSCs into bone-resorbing osteoclasts based upon a monoculture approach consisting of three steps. First, based on conventional hanging-drop methods, embryoid bodies (EBs) were produced from mouse ESCs or iPSCs. Second, EBs were cultured in medium supplemented with macrophage colony-stimulating factor (M-CSF), and differentiated to osteoclast precursors, which expressed CD11b. Finally, ESC- or iPSC-derived osteoclast precursors stimulated with receptor activator of nuclear factor-B ligand (RANKL) and M-CSF formed large multinucleated osteoclast-like cells that expressed tartrate-resistant acid phosphatase and were capable of bone resorption. Molecular analysis showed that the expression of osteoclast marker genes such as Nfatc1, Ctsk, and Acp5 are increased in a RANKL-dependent manner. Thus, our procedure is simple and easy and would be helpful for stem cell-based bone research.
  • Journal of bone and mineral metabolism.J Bone Miner Metab.2013 Dec 24. [Epub ahead of print]
  • The development of methods for differentiation of embryonic stem cells (ESCs) and induced pluripotent stem cell (iPSCs) into functional cells have helped to analyze the mechanism regulating cellular processes and to explore cell-based assays for drug discovery. Although several reports have demonstr
  • PMID 24366621
  • One-step microfluidic generation of pre-hatching embryo-like core-shell microcapsules for miniaturized 3D culture of pluripotent stem cells.
  • Agarwal P, Zhao S, Bielecki P, Rao W, Choi JK, Zhao Y, Yu J, Zhang W, He X.Author information Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA. He.429@osu.edu.AbstractA novel core-shell microcapsule system is developed in this study to mimic the miniaturized 3D architecture of pre-hatching embryos with an aqueous liquid-like core of embryonic cells and a hydrogel-shell of zona pellucida. This is done by microfabricating a non-planar microfluidic flow-focusing device that enables one-step generation of microcapsules with an alginate hydrogel shell and an aqueous liquid core of cells from two aqueous fluids. Mouse embryonic stem (ES) cells encapsulated in the liquid core are found to survive well (>92%). Moreover, ~20 ES cells in the core can proliferate to form a single ES cell aggregate in each microcapsule within 7 days while at least a few hundred cells are usually needed by the commonly used hanging-drop method to form an embryoid body (EB) in each hanging drop. Quantitative RT-PCR analyses show significantly higher expression of pluripotency marker genes in the 3D aggregated ES cells compared to the cells under 2D culture. The aggregated ES cells can be efficiently differentiated into beating cardiomyocytes using a small molecule (cardiogenol C) without complex combination of multiple growth factors. Taken together, the novel 3D microfluidic and pre-hatching embryo-like microcapsule systems are of importance to facilitate in vitro culture of pluripotent stem cells for their ever-increasing use in modern cell-based medicine.
  • Lab on a chip.Lab Chip.2013 Dec 7;13(23):4525-33. doi: 10.1039/c3lc50678a.
  • A novel core-shell microcapsule system is developed in this study to mimic the miniaturized 3D architecture of pre-hatching embryos with an aqueous liquid-like core of embryonic cells and a hydrogel-shell of zona pellucida. This is done by microfabricating a non-planar microfluidic flow-focusing dev
  • PMID 24113543
  • A high-throughput-compatible 3D microtissue co-culture system for phenotypic RNAi screening applications.
  • Thoma CR, Stroebel S, Rösch N, Calpe B, Krek W, Kelm JM.Author information 1Institute of Molecular Health Sciences, ETH Zurich, Zurich, Switzerland.AbstractCancer cells in vivo are coordinately influenced by an interactive 3D microenvironment. However, identification of drug targets and initial target validations are usually performed in 2D cell culture systems. The opportunity to design 3D co-culture models that reflect, at least in part, these heterotypic interactions, when coupled with RNA interference, would enable investigations on the phenotypic impact of gene function in a model that more closely resembles tumor growth in vivo. Here we describe a high-throughput-compatible method to discover cancer gene functions in a co-culture 3D tumor microtissue model system composed of human DLD1 colon cancer cells together with murine fibroblasts. Strikingly, DLD1 cells in this model failed to expand upon siRNA-mediated depletion of Kif11/Eg5, a member of the mitotic kinesin-like motor protein family. In contrast, these cancer cells proved to be more resistant to Kif11/Eg5 depletion when grown as a 2D monolayer. These results suggest that growth of certain cancer cells in 3D versus 2D can unveil differential dependencies on specific genes for their survival. Moreover, they denote that the high-throughput-compatible, hanging drop technology-based 3D co-culture model will enable the discovery, characterization, and validation of gene functions in key biological and pathological processes.
  • Journal of biomolecular screening.J Biomol Screen.2013 Dec;18(10):1330-7. doi: 10.1177/1087057113499071. Epub 2013 Sep 30.
  • Cancer cells in vivo are coordinately influenced by an interactive 3D microenvironment. However, identification of drug targets and initial target validations are usually performed in 2D cell culture systems. The opportunity to design 3D co-culture models that reflect, at least in part, these hetero
  • PMID 24080258

Japanese Journal

  • Three Dimensional Spheroid Culture of Canine Amniotic Fluid Derived Mesenchymal Stem Cells Enhances Differentiation Efficacycy
  • 九州大学大学院農学研究院紀要 60(2), 377-384, 2015-09-18
  • NAID 120005661372
  • P-54 ES細胞分化による心筋収縮リズムへの多層カーボンナノチューブ(MWCNTs)の影響(インプラント・生体組織・毒性,一般講演(ポスター発表),第65回日本歯科理工学会学術講演会)
  • Influence of MWCNTs to Myocardial Contraction Rhythms on Differentiation of ES-D3 Cells in Three-dimensional Culture
★リンクテーブル★
関連記事「drop」「culture」

「drop」

　　[★]

  • n.
  • vt.
  • vi.
  • (液体が)したたる、ぼたぼた落ちる
  • (物が)(急に、思いがけなく)落ちる(down)。(花が)散る。(人が)(車などから)ひらりと(飛び)降りる(off)。(正式)(川・丘などが)下る(down)
  • (人が)(疲れて・傷ついて)急に倒れる。死ぬ。(意識的に)身を伏せる
  • (程度・数量・価値などが)下がる(fall)、落ちる(away)。健康や病状が悪化する
  • (事が)やめになる。(～から)手を引く、やめる(from)。(視界から)見えなくなる(out of, from)。(交通・話などが)とだえる(off)
  • 急に(ある状態に)なる、陥る(into, to)
  • (略式)ひょいと(～に)立ち寄る(in, by, over, round)(on, at, into)。(人に)偶然であう(in)(across, on, with)
  • 落後(後退)する、遅れる(away, back, off, behind)
  • (動物が)生まれる
  • (猟犬が)獲物を見て伏せる
  • (言葉・ため息などが)(～から)ふともれる(from)
  • (水・空気の流れに沿って)穏やかに動く
  • (トランプ)(ブリッジなどで)(特定の札が)出るはめになる、死ぬ
  • (球技)(球が)ホール(ポケット、ゴール)に入る(転がり込む)
  • 逮捕される
関
decline, decrease, depression, droplet, dropping, dropwise, fall, instill, instillation, lower, lowering, reduction

　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　 　

「culture」

　　[★]

  • n.

　 　 　 　 　 　 　

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