出典: meddic


reverse transcriptase-PCRreverse transcriptase-polymerase chain reactionreverse transcription-PCRreverse transcription-polymerase chain reaction
reverse transcriptase polymerase chain reaction

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出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2015/05/28 17:16:44」(JST)

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  • Low-dose gold nanoparticles exert subtle endocrine-modulating effects on the ovarian steroidogenic pathway ex vivo independent of oxidative stress.
  • Larson JK1, Carvan MJ 3rd, Teeguarden JG, Watanabe G, Taya K, Krystofiak E, Hutz RJ.
  • Nanotoxicology.Nanotoxicology.2014 Dec;8(8):856-66. doi: 10.3109/17435390.2013.837208.
  • Gold nanoparticles (GNPs) have gained considerable attention for application in science and industry. However, the untoward effects of such particles on female fertility remain unclear. The objectives of this study were to (1) examine the effects of 10-nm GNPs on progesterone and estradiol-17β accu
  • PMID 23992423
  • Strongly structured DNA sequences as targets for genosensing: Sensing phase design and coupling to PCR amplification for a highly specific 33-mer gliadin DNA fragment.
  • Martín-Fernández B1, Miranda-Ordieres AJ2, Lobo-Castañón MJ3, Frutos-Cabanillas G4, de-Los-Santos-Álvarez N5, López-Ruiz B6.
  • Biosensors & bioelectronics.Biosens Bioelectron.2014 Oct 15;60:244-51. doi: 10.1016/j.bios.2014.04.033. Epub 2014 Apr 30.
  • Electrochemical genosensors are becoming cost-effective miniaturizable alternatives to real-time PCR (RT-PCR) methods for the detection of sequence-specific DNA fragments. We report on the rapid detection of PCR amplicons without the need of purification or strand separation. A challenging target se
  • PMID 24813914
  • Production of healthy cloned pigs with neural stem cells as nuclear donors.
  • Li Q1, Wang H, Zhou GB, Jinfei M, Yan L, Zhaihu Z, Li N.
  • Animal biotechnology.Anim Biotechnol.2014 Oct 2;25(4):294-305. doi: 10.1080/10495398.2013.872119.
  • The objectives of the present study were to establish a porcine neural stem cell (NSC) line and to determine if these NSCs could be used to produce cloned pigs. NSCs were isolated from the brains of three embryonic day 30 fetal pigs and were induced to differentiate in vitro . NSCs and the different
  • PMID 24813221


  • Electrofusion of Mesenchymal Stem Cells and Islet Cells for Diabetes Therapy: A Rat Model
  • Yanai Goichi,Hayashi Takashi,Zhi Qi,Yang Kai-Chiang,Shirouzu Yasumasa,Shimabukuro Takashi,Hiura Akihito,Inoue Kazutomo,Sumi Shoichiro
  • PLoS ONE 8(5), 2013-05-28
  • … In the fusion cells between rat islet cells and mouse MSCs, RT-PCR showed new expression of both rat MSC-related genes and mouse β-cell-related genes, indicating bidirectional reprogramming of both β-cell and MSCs nuclei. …
  • NAID 120005245076
  • Sustained Repression and Translocation of NTCP and Expression of MRP4 for Cholestasis after Rat 90% Partial Hepatectomy
  • Miura Takuya,Kimura Norihisa,Yamada Toshiyuki,Shimizu Takeshi,Nanashima Naoki,Yamana Daisuke,Hakamada Kenichi,Tsuchida Shigeki
  • 弘前医学 64(Supplement), S99-S106, 2013-04-02
  • … anion transporters for bile acids was examined in a rat model.Methods: Male Sprague-Dawley rats were subjected to 90% hepatectomy, and tissues were harvested on 0, 1, 3, and 7 days for microarray analysis, the quantitative real-time polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry to examine the expression of multidrug resistance protein 4 (Mrp4), bile salt export pump( Bsep) and sodium-dependent taurocholate cotransporting polypeptide( Ntcp).Results: Persistently elevated serum …
  • NAID 120005230059
  • Differentiation Inhibition of Regulatory T Cells in Hirosaki Hairless Rat Due to Deletion of The LY49 Family Genes Expressed in Thymic T Cells and Dendritic Cells
  • Yamada Toshiyuki,Nanashima Naoki,Shimizu Takeshi,Tsuchida Shigeki
  • 弘前医学 64(Supplement), S65-S69, 2013-04-02
  • … Real-time PCR revealed reduced expression of the Foxp3 and CD25 (IL-2Rα) genes, important for nTreg differentiation, and flow cytometric analysis showed decreased number of CD4+CD25+Foxp3+nTreg cells, indicating that mature nTreg formation is repressed. …
  • NAID 120005230053


RT-PCR法 RTとはReverse Transcriptionの略で、逆転写を意味します。 逆転写とは、RNAを鋳型としてDNAを合成することで、逆転写により合成されたDNAをcDNA(cは“相補的”を意味する“complementary”の頭文字です。)と呼びます。 RT ...
PCR / RT-PCR法 PCR法は、ゲノムDNAなどをテンプレートにして、増幅したい領域の両端に相補的なプライマーと耐熱性DNAポリメラーゼを用いてサイクル反応(プライマーとのアニーリング→伸長反応→二本鎖の解離)を行うことにより、目的 ...


 of mRNA Using Real-Time RT-PCRRT-PCR Biomol: Reverse Transcription PCR (RT-PCR Biomol: Reverse Transcription PCR (RT-PCRRT-PCR lab - PCR实验 - 生物秀 flow left and mirna rt pcr mechanism right

リンク元逆転写PCR」「reverse transcription-PCR」「reverse transcriptase-PCR」「reverse transcriptase-polymerase chain reaction」「reverse transcriptase polymerase chain reaction



reverse transcription-polymerase chain reactionreverse transcriptase-polymerase chain reactionreverse transcription-PCRreverse transcriptase-PCRRT-PCR
reverse transcriptase polymerase chain reaction

reverse transcription-PCR」


reverse transcriptase-PCRreverse transcriptase-polymerase chain reactionreverse transcription-polymerase chain reactionRT-PCR

reverse transcriptase-PCR」


reverse transcriptase-polymerase chain reactionreverse transcription-PCRreverse transcription-polymerase chain reactionRT-PCR

reverse transcriptase-polymerase chain reaction」



reverse transcriptase-PCRreverse transcription-PCRreverse transcription-polymerase chain reactionRT-PCR

reverse transcriptase polymerase chain reaction」








quantitative real-time PCR



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  [★] ポリメラーゼ連鎖反応 polymerase chain reaction

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