医療技術評価 medical technology assessment
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- the 13th letter of the Roman alphabet (同)m
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- Mach number / mark[s] / Monsieur
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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/10/12 22:11:55」(JST)
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- 1. 小児および思春期における注意欠陥多動性障害：治療および予後の概要 attention deficit hyperactivity disorder in children and adolescents overview of treatment and prognosis
English Journal
- In-situ and label-free optical monitoring of the adhesion and spreading of primary monocytes isolated from human blood: dependence on serum concentration levels.
- Orgovan N1, Salánki R2, Sándor N3, Bajtay Z4, Erdei A5, Szabó B6, Horvath R7.Author information 1Department of Biological Physics, Eötvös Loránd University, Pázmány P. stny. 1/A, H-1117 Budapest, Hungary; Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Konkoly-Thege út 29-33, H-1120 Budapest, Hungary.2Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Konkoly-Thege út 29-33, H-1120 Budapest, Hungary; Doctoral School of Molecular- and Nanotechnologies, University of Pannonia, Veszprém, Hungary.3MTA-ELTE Immunology Research Group, ELTE, Pázmány P. stny. 1/C, H-1117 Budapest, Hungary.4Department of Immunology, Eötvös Loránd University, Pázmány P. stny. 1/C, H-1117 Budapest, Hungary.5MTA-ELTE Immunology Research Group, ELTE, Pázmány P. stny. 1/C, H-1117 Budapest, Hungary; Department of Immunology, Eötvös Loránd University, Pázmány P. stny. 1/C, H-1117 Budapest, Hungary.6Department of Biological Physics, Eötvös Loránd University, Pázmány P. stny. 1/A, H-1117 Budapest, Hungary.7Nanobiosensorics Group, Hungarian Academy of Sciences, Research Centre for Natural Sciences, Institute for Technical Physics and Materials Science, Konkoly-Thege út 29-33, H-1120 Budapest, Hungary. Electronic address: horvathr@mfa.kfki.hu.AbstractAdhesion and spreading of primary monocytes isolated from human blood were monitored utilizing optical waveguide lightmode spectroscopy (OWLS); a highly sensitive label-free biosensor technique using evanescent optical waves generated at a biocompatible surface. Appropriate development on a custom built setup enabled the OWLS cuvette to be operated as a 1.5 ml mini-incubator, controlling both temperature and CO2 levels. The incubator-equipped OWLS is readily applicable for delicate and long-term studies on sensitive primary cells, demonstrated here through monitoring the serum dependence of the adhesion and spreading of human monocytes. Moreover, the custom-built setup enables the simultaneous monitoring of the position and overall width of the OWLS resonant peaks. This unique feature makes it possible to distinguish the refractive index variations induced by the adsorption of secreted material from refractive index changes provoked by cellular spreading. A definite attachment and spreading activity was observed on the substratum (glassy silica-titania), when the serum level of the culturing medium was 0.0-0.01%. Increasing serum concentration resulted in a steep fall in monocyte surface adhesion and spreading. 1.0% serum level practically abolished all spreading activity measured by OWLS, and the number of attached cells was significantly decreased, too. Serum addition to fully spread cells provoked a reduction in the cell-substratum contact area, clearly detectable by the biosensor. Cell spreading was inhibited by pre-coating the sensor surface with considerable amounts of serum proteins. These findings suggest that monocyte spreading is inhibited by the adsorption of serum biomolecules to the substratum, rather than by soluble factors present in the serum. All of these results were obtained completely noninvasively with real time monitoring; demonstrating the capabilities of OWLS to sensitively monitor the adhesion properties of immune cells isolated from human blood. The current study is, therefore, a significant step towards the application of label-free optical biosensors in medical diagnostics.
- Biosensors & bioelectronics.Biosens Bioelectron.2014 Apr 15;54:339-44. doi: 10.1016/j.bios.2013.10.076. Epub 2013 Nov 12.
- Adhesion and spreading of primary monocytes isolated from human blood were monitored utilizing optical waveguide lightmode spectroscopy (OWLS); a highly sensitive label-free biosensor technique using evanescent optical waves generated at a biocompatible surface. Appropriate development on a custom b
- PMID 24291753
- Identification of motives mediating alternative functions of the neomorphic moonlighting TPPP/p25.
- Tőkési N1, Oláh J2, Hlavanda E3, Szunyogh S4, Szabó A5, Babos F6, Magyar A7, Lehotzky A8, Vass E9, Ovádi J10.Author information 1Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, 1113 Budapest, Hungary. Electronic address: tokesi.natalia@ttk.mta.hu.2Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, 1113 Budapest, Hungary. Electronic address: olah.judit@ttk.mta.hu.3Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, 1113 Budapest, Hungary. Electronic address: hlavanda.emma@gmail.com.4Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, 1113 Budapest, Hungary. Electronic address: szunyogh@enzim.hu.5Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, 1113 Budapest, Hungary. Electronic address: szaboadel90@gmail.com.6Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, 1117 Budapest, Hungary. Electronic address: babosfruzsi@gmail.com.7Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, 1117 Budapest, Hungary. Electronic address: magyar@chem.elte.hu.8Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, 1113 Budapest, Hungary. Electronic address: lehotzky.attila@ttk.mta.hu.9Department of Organic Chemistry, Institute of Chemistry, Eötvös Loránd University, 1117 Budapest, Hungary. Electronic address: evass@chem.elte.hu.10Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, 1113 Budapest, Hungary. Electronic address: ovadi.judit@ttk.mta.hu.AbstractThe disordered Tubulin Polymerization Promoting Protein (TPPP/p25), a prototype of neomorphic moonlighting proteins, displays physiological and pathological functions by interacting with distinct partners. Here the role of the disordered N- and C-termini straddling a middle flexible segment in the distinct functions of TPPP/p25 was established, and the binding motives responsible for its heteroassociations with tubulin and α-synuclein, its physiological and pathological interacting partner, respectively, were identified. We showed that the truncation of the disordered termini altered the folding state of the middle segment and has functional consequences concerning its physiological function. Double truncation diminished its binding to tubulin/microtubules, consequently the tubulin polymerization/microtubule bundling activities of TPPP/p25 were lost highlighting the role of the disordered termini in its physiological function. In contrast, interaction of TPPP/p25 with α-synuclein was not affected by the truncations and its α-synuclein aggregation promoting activity was preserved, showing that the α-synuclein binding motif is localized within the middle segment. The distinct tubulin and α-synuclein binding motives of TPPP/p25 were also demonstrated at the cellular level: the double truncated TPPP/p25 did not align along the microtubules in contrast to the full length form, while it induced α-synuclein aggregation. The localization of the binding motives on TPPP/p25 were established by specific ELISA experiments performed with designed and synthesized peptides: motives at the 178-187 and 147-156 segments are involved in the binding of tubulin and α-synuclein, respectively. The dissimilarity of these binding motives responsible for the neomorphic moonlighting feature of TPPP/p25 has significant innovative impact in anti-Parkinson drug research.
- Biochimica et biophysica acta.Biochim Biophys Acta.2014 Apr;1842(4):547-57. doi: 10.1016/j.bbadis.2014.01.009. Epub 2014 Jan 23.
- The disordered Tubulin Polymerization Promoting Protein (TPPP/p25), a prototype of neomorphic moonlighting proteins, displays physiological and pathological functions by interacting with distinct partners. Here the role of the disordered N- and C-termini straddling a middle flexible segment in the d
- PMID 24463170
- Deletion of PARP-2 induces hepatic cholesterol accumulation and decrease in HDL levels.
- Szántó M1, Brunyánszki A2, Márton J3, Vámosi G4, Nagy L3, Fodor T3, Kiss B5, Virág L1, Gergely P3, Bai P6.Author information 1MTA-DE Cell Biology and Signaling Research Group of the Hungarian Academy of Sciences, 4032 Debrecen, Hungary; Department of Medical Chemistry, University of Debrecen, 4032 Debrecen, Hungary.2MTA-DE Cell Biology and Signaling Research Group of the Hungarian Academy of Sciences, 4032 Debrecen, Hungary.3Department of Medical Chemistry, University of Debrecen, 4032 Debrecen, Hungary.4Department of Biophysics and Cell Biology, University of Debrecen, 4032 Debrecen, Hungary.5Department of Dermatology, Medical and Health Science Center, University of Debrecen, 4032 Debrecen, Hungary.6MTA-DE Cell Biology and Signaling Research Group of the Hungarian Academy of Sciences, 4032 Debrecen, Hungary; Department of Medical Chemistry, University of Debrecen, 4032 Debrecen, Hungary. Electronic address: baip@med.unideb.hu.AbstractPoly(ADP-ribose) polymerase-2 (PARP-2) is acknowledged as a DNA repair enzyme. However, recent investigations have attributed unique roles to PARP-2 in metabolic regulation in the liver. We assessed changes in hepatic lipid homeostasis upon the deletion of PARP-2 and found that cholesterol levels were higher in PARP-2(-/-) mice as compared to wild-type littermates. To uncover the molecular background, we analyzed changes in steady-state mRNA levels upon the knockdown of PARP-2 in HepG2 cells and in murine liver that revealed higher expression of sterol-regulatory element binding protein (SREBP)-1 dependent genes. We demonstrated that PARP-2 is a suppressor of the SREBP1 promoter, and the suppression of the SREBP1 gene depends on the enzymatic activation of PARP-2. Consequently, the knockdown of PARP-2 enhances SREBP1 expression that in turn induces the genes driven by SREBP1 culminating in higher hepatic cholesterol content. We did not detect hypercholesterolemia, higher fecal cholesterol content or increase in serum LDL, although serum HDL levels decreased in the PARP-2(-/-) mice. In cells and mice where PARP-2 was deleted we observed decreased ABCA1 mRNA and protein expression that is probably linked to lower HDL levels. In our current study we show that PARP-2 impacts on hepatic and systemic cholesterol homeostasis. Furthermore, the depletion of PARP-2 leads to lower HDL levels which represent a risk factor to cardiovascular diseases.
- Biochimica et biophysica acta.Biochim Biophys Acta.2014 Apr;1842(4):594-602. doi: 10.1016/j.bbadis.2013.12.006. Epub 2013 Dec 21.
- Poly(ADP-ribose) polymerase-2 (PARP-2) is acknowledged as a DNA repair enzyme. However, recent investigations have attributed unique roles to PARP-2 in metabolic regulation in the liver. We assessed changes in hepatic lipid homeostasis upon the deletion of PARP-2 and found that cholesterol levels we
- PMID 24365238
Japanese Journal
- INTERDISCIPLINARY APPROACH 土木工学的観点からMTAを考察する
- 日本歯科評論 76(6), 89-102, 2016-06
- NAID 40020862166
- インタビュー マイケル・ホロッドニチアノ氏(MTAキャピタル・コンストラクション社長) 水は完全に封じず適切に排水する (トンネル 2番街地下鉄建設工事(米国・ニューヨーク市) 巨大都市の地下を発破で掘る)
- 日経コンストラクション (634), 19-21, 2016-02-22
- NAID 40020737346
- 歯学の進歩・現状 歯内療法 : 臨床の現状とジレンマ
- 歯科学報 = The journal of the Tokyo Dental College Society 116(1), 1-4, 2016-02
- NAID 120005726939
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| リンク元 | 「医療技術評価」 |
| 拡張検索 | 「EMTALA」 |
| 関連記事 | 「MT」「M」 |
「医療技術評価」
参考
- 医療技術評価の使用の国際動向
- International Trends in the Use of Health Technology Assessment
- 費用効率の検討を活用して新しい医薬品や医療技術の相対価値を評価する医療技術評価(HTA)は、償還すべき治療法を償還当局が決定する上で重要な役割を担うものである。オーストラリア、カナダ、及びヨーロッパ諸国の大半の償還当局は、ここ数年間、その償還決定にHTAを利用してきたし、今や多くの国がそれに続いている。重要なことに、アメリカの支払者も、償還決定におけるHTAの可能性に興味を持ち始めている。
「EMTALA」
- 同
- Emergency Medical Treatment and Active Labor Act
「MT」