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Yamakoshi Y1, Simmer JP, Bartlett JD, Karakida T, Oida S.
Archives of oral biology.Arch Oral Biol.2013 Nov;58(11):1569-77. doi: 10.1016/j.archoralbio.2013.08.005. Epub 2013 Aug 18.
OBJECTIVE: Enamelysin (MMP20) and kallikrein 4 (KLK4) are believed to be necessary to clear proteins from the enamel matrix of developing teeth. MMP20 is expressed by secretory stage ameloblasts, while KLK4 is expressed from the transition stage throughout the maturation stage. The aim of this study
PMID 24112721

Can Edman degradation be used for quantification? Isotope-dilution liquid chromatography-electrospray ionization tandem mass spectrometry and the long-term stability of 20 phenylthiohydantoin-amino acids.

Satoh R1, Goto T, Lee SH, Oe T.
Analytical and bioanalytical chemistry.Anal Bioanal Chem.2013 Oct;405(25):8001-10. doi: 10.1007/s00216-013-6901-6. Epub 2013 Apr 2.
Edman degradation is a well-known method for obtaining amino acid (AA) sequences from a peptide by means of sequential reactions that release the N-terminal AAs from the peptide as a phenylthiohydantoin (PTH) derivative. Because of unexpected loss during the reaction and handling, there are few repo
PMID 23545858

Lucifensin II, a defensin of medicinal maggots of the blowfly Lucilia cuprina (Diptera: Calliphoridae).

El Shazely B1, Veverka V, Fucík V, Voburka Z, Zdárek J, Cerovský V.
Journal of medical entomology.J Med Entomol.2013 May;50(3):571-8.
A novel homolog of insect defensin, designated lucifensin II (Lucilia cuprina Wiedemann [Diptera: Calliphoridae] defensin), was purified from hemolymph extract from larvae of the blowfly L. cuprina. The full-length primary sequence of this peptide of 40 amino acid residues and three intramolecular d
PMID 23802451

A manual sequence method of peptides and phosphopeptides using 4-(1'-cyanoisoindolyl)phenylisothiocyanate.

Shibata Takayuki,Wainaina Moses N,Miyoshi Takayuki,Kabashima Tsutomu,Kai Masaaki
Journal of Chromatography. A 1218(24), 3757-3762, 2011-06-17
… The peptides were derivatized with an Edman type reagent, 4-(1'-cyanoisoindolyl)phenylisothiocyanate (CIPIC) and subsequently cleaved to generate stable and fluorescent 4-(1'-cyanoisoindolyl)phenylthiazolinone (CIP-TZ)-amino acids. … The results demonstrated the potential of CIPIC as a derivatization reagent for peptide sequencing and the applicability of the method for the study and identification of phosphoamino acids in peptides. …
NAID 120003220737

三田智文
藥學雜誌 118(11), 483-492, 1998-11-01
… The relationship enabled to design new fluorogenic reagents easily Secondly, the simultaneous determination method for the sequence and D/L configuration of peptides with fluorescent Edman reagent was developed. … In this study, the racemization mechanism of amino acids in the cleavage/cyclization step was investigated and boron trifluoride was employed as cleavage/cyclization reagent in order to supress the racemization. …
NAID 110003649123

Gas-phase Microsequencing of Peptides and Proteins with a Fluorescent Edman-type Reagent, Fluorescein Isothiocyanate

Muramoto Koji,Nokihara Kiyoshi,Ueda Akira,Kamiya Hisao
Bioscience, Biotechnology, and Biochemistry 58(2), 300-304, 1994-02-23
… A fluorescent Edman-type reagent, fluorescein isothiocyanate (FITC), was adapted to a gas-phase sequencer fitted with a miniature reaction chamber. …
NAID 110002676808