[The effect of up-regulated expression of Rap1GAP on the invasion ability of HL-60 cells in vitro and in vivo].
Qiu T1, Li D1, Li Z1, Xu K1, Qi X1, Cen J1, Chen Z1.
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi.Zhonghua Xue Ye Xue Za Zhi.2015 Jul;36(7):570-4. doi: 10.3760/cma.j.issn.0253-2727.2015.07.009.
OBJECTIVE: To investigate the effect of up-regulation of Rap1GAP on the invasion ability of leukemic HL-60 cells in vitro, and to establish leukemia mouse model to verify the effects in vivo.METHODS: Quantitative RT-PCR and Western blot methods were used to detect the expression of Rap1GAP in Venus/
Differential activation of Wnt-β-catenin pathway in triple negative breast cancer increases MMP7 in a PTEN dependent manner.
Dey N1, Young B, Abramovitz M, Bouzyk M, Barwick B, De P, Leyland-Jones B.
PloS one.PLoS One.2013 Oct 15;8(10):e77425. doi: 10.1371/journal.pone.0077425. eCollection 2013.
Mutations of genes in tumor cells of Triple Negative subset of Breast Cancer (TNBC) deregulate pathways of signal transduction. The loss of tumor suppressor gene PTEN is the most common first event associated with basal-like subtype (Martins, De, Almendro, Gonen, and Park, 2012). Here we report for
Loxoscelism is the designation given to clinical symptoms evoked by Loxosceles spider's bites. Clinical manifestations include skin necrosis with gravitational spreading and systemic disturbs. The venom contains several enzymatic toxins. Herein, we describe the cloning, expression, refolding and bio
General Procedure [edit] Gel zymography [edit] Samples are prepared in a standard, non-reducing loading buffer for SDS-PAGE. No reducing agent or boiling are necessary since these would interfere with refolding of the enzyme. A ...