キシレンシアノール
WordNet
- a primary subtractive color for light; has a blue-green color
- a colorless flammable volatile liquid hydrocarbon used as a solvent (同)xylol
PrepTutorEJDIC
- 青緑色,シアン
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/05/11 17:11:01」(JST)
[Wiki en表示]
Xylene cyanol
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| Names |
Other names
Acid Blue 147
xylene cyanole
xylene cyanol FF
xylene cyanole FF
C.I. 42135 |
| Identifiers |
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CAS Number
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2650-17-1 N |
| ChemSpider |
21106494 Y |
| Jmol 3D model |
Interactive image |
InChI
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InChI=1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(32-34(28)29)15-24(21)33-35(30)31;/h7-15,26H,5-6H2,1-4H3,(H,28,29)(H,30,31);/q;+1/p-1/b25-19-,27-23-; Y
Key: NLIVDORGVGAOOJ-KRQUPCAFSA-M Y
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InChI=1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(32-34(28)29)15-24(21)33-35(30)31;/h7-15,26H,5-6H2,1-4H3,(H,28,29)(H,30,31);/q;+1/p-1/b25-19-,27-23-;
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Key: NLIVDORGVGAOOJ-KRQUPCAFSA-M
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SMILES
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[Na+].CCNc1ccc(cc1C)/C(=C2/C=C\C(=[NH+]\CC)C(=C2)C)c3ccc(OS([O-])=O)cc3OS([O-])=O
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| Properties |
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Chemical formula
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C25H27N2NaO6S2 |
| Molar mass |
538.61 g/mol |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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| N verify (what is YN ?) |
| Infobox references |
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Xylene cyanol can be used as a color marker, or tracking dye, to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Bromophenol blue and orange G can also be used for this purpose.
Once mixed with the sample, concentration of xylene cyanol is typically about 0.005% to 0.03%.
Contents
- 1 Migration speed
- 2 Conjugation
- 3 References
- 4 External links
Migration speed
In 1% agarose gels, xylene cyanol migrates at about the same rate as a 4 to 5 kilobase pair DNA fragment [1] (although this depends on the buffer used). Xylene cyanol on a 6% polyacrylamide gel migrates at the speed of a 140 base pair DNA fragment. On 20% denaturating (7 M urea) polyacrylamide gel electrophoresis (PAGE), xylene cyanol migrates at about the rate of 25 bases oligonucleotide.
Conjugation
Due to its large number of double bonds separated by single bonds, the molecule is said to be highly conjugated.
References
- ^ Lela Buckingham & Maribeth L. Flaws. Molecular Diagnostics.- Fundamentals, Methods, & Clinical Applications. F.A. Davis Company, 2007, p. 91
External links
- Xylene cyanol at OpenWetWare
UpToDate Contents
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English Journal
- Non-emissive plastic colour filters for fluorescence detection.
- Yamazaki M1, Krishnadasan S, deMello AJ, deMello JC.
- Lab on a chip.Lab Chip.2012 Nov 7;12(21):4313-20. doi: 10.1039/c2lc40718c.
- We report the fabrication of non-emissive short- and long-pass filters on plastic for high sensitivity fluorescence detection. The filters were prepared by overnight immersion of titania-coated polyethylene terephthalate (PET) in an appropriate dye solution - xylene cyanol for short-pass filtering a
- PMID 22971690
- Retraction: Simultaneous determination of trace iron and aluminum by catalytic spectrophotometry based on a novel oxidation reaction of xylene cyanol FF.
- [No authors listed]
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.Anal Sci.2009 Jul;25(7):949.
- PMID 19609039
- Simultaneous determination of trace iron and aluminum by catalytic spectrophotometry based on a novel oxidation reaction of xylene cyanol FF.
- Cai L1, Xu C.
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.Anal Sci.2008 Jun;24(6):775-8.
- A new, simple, sensitive and selective method for the simultaneous determination of trace iron and aluminum by catalytic spectrophotometry was presented, based on the catalytic effects of iron and aluminum on the discoloring reaction of xylene cyanol FF proceeded by hydrogen peroxide and potassium p
- PMID 18544868
Japanese Journal
- Retraction: Simultaneous Determination of Trace Iron and Aluminum by Catalytic Spectrophotometry Based on a Novel Oxidation Reaction of Xylene Cyanol FF
- CAI Longfei,XU Chunxiu
- Analytical Sciences 25(7), 949-949, 2009
- This article published in Anal. Sci., 2008, 24(6), 775 was withdrawn by the Editor on May 11, 2009 due to improper authorship in its writing.
- NAID 130004441924
- Simultaneous Determination of Trace Iron and Aluminum by Catalytic Spectrophotometry Based on a Novel Oxidation Reaction of Xylene Cyanol FF
- Cai Longfei,Xu Chunxiu
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 24(6), 775-778, 2008-06-10
- This article was retracted. See the retraction.
- NAID 10025204530
- Spectrophotometric Determination of Cerium with Leuco Xylene Cyanol FF
- Revanasiddappa Hosakere D.,Kiran Kumar Telangusadhushetty N.
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 18(11), 1275-1278, 2002-11-10
- NAID 10011310438
Related Links
- Xylene cyanol (XC) is most commonly used as an agarose gel electrophoresis size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it ...
- Xylene cyanol can be used as a colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Bromophenol blue and orange G can also be used for this purpose. Chemistry educational resource for Xylene cyanol (standard InChIKey NLIVDORGVGAOOJ-KRQUPCAFSA-M).
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- 関
- blue、CN、cyanogen