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- いうことを聞かない,強情な,わがままな / 気まぐれな,むら気な
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- 1. 下肢末梢神経症候群の概要 overview of lower extremity peripheral nerve syndromes
English Journal
- China to blacklist wayward drug manufacturers.
- Parry J.
- BMJ (Clinical research ed.).BMJ.2014 Jan 14;348:g243. doi: 10.1136/bmj.g243.
- PMID 24424287
- Real-time single-molecule tethered particle motion analysis reveals mechanistic similarities and contrasts of Flp site-specific recombinase with Cre and λ Int.
- Fan HF1, Ma CH, Jayaram M.Author information 1Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei 112, Taiwan. hffan2@ym.edu.twAbstractFlp, a tyrosine site-specific recombinase coded for by the selfish two micron plasmid of Saccharomyces cerevisiae, plays a central role in the maintenance of plasmid copy number. The Flp recombination system can be manipulated to bring about a variety of targeted DNA rearrangements in its native host and under non-native biological contexts. We have performed an exhaustive analysis of the Flp recombination pathway from start to finish by using single-molecule tethered particle motion (TPM). The recombination reaction is characterized by its early commitment and high efficiency, with only minor detraction from 'non-productive' and 'wayward' complexes. The recombination synapse is stabilized by strand cleavage, presumably by promoting the establishment of functional interfaces between adjacent Flp monomers. Formation of the Holliday junction intermediate poses a rate-limiting barrier to the overall reaction. Isomerization of the junction to the conformation favoring its resolution in the recombinant mode is not a slow step. Consistent with the completion of nearly every initiated reaction, the chemical steps of strand cleavage and exchange are not reversible during a recombination event. Our findings demonstrate similarities and differences between Flp and the mechanistically related recombinases λ Int and Cre. The commitment and directionality of Flp recombination revealed by TPM is consistent with the physiological role of Flp in amplifying plasmid DNA.
- Nucleic acids research.Nucleic Acids Res.2013 Aug;41(14):7031-47. doi: 10.1093/nar/gkt424. Epub 2013 Jun 3.
- Flp, a tyrosine site-specific recombinase coded for by the selfish two micron plasmid of Saccharomyces cerevisiae, plays a central role in the maintenance of plasmid copy number. The Flp recombination system can be manipulated to bring about a variety of targeted DNA rearrangements in its native hos
- PMID 23737451
- Suicide gene-mediated ablation of tumor-initiating mouse pluripotent stem cells.
- Chen F1, Cai B, Gao Y, Yuan X, Cheng F, Wang T, Jiang M, Zhou Y, Lahn BT, Li W, Xiang AP.Author information 1Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, Guangdong 510080, PR China.AbstractPluripotent stem cells, including embryonic stem (ES) and induced pluripotent stem (iPS) cells, serve as unlimited resources for cell replacement therapy and tissue engineering because such cells are capable of extensive proliferation in vitro and can give rise to lineages that represent any of the three embryonic germ layers. However, in the context of the in vivo behavior of cell transplants, key challenges need to be addressed and essential strategies should be developed before stem cells can be used in clinical practice. In the present study, we modified mouse ES/iPS cells to contain a suicide gene, deltaTK or CodA, under the transcriptional control of the EF1α or Nanog promoter. The suicide gene was introduced via lentivirus transduction without interfering with their self-renewal and pluripotency characteristics. We found that EF1α promoter-controlled deltaTK/CodA expression efficiently eliminated pluripotent stem cells and their derivatives both in vitro and in vivo. When the suicide gene was under the control of the Nanog promoter, tumor-initiating undifferentiated pluripotent stem cells were selectively ablated in vitro after prodrug treatment. These results indicate that modification of pluripotent stem cells with a suicide gene prior to transplantation offers a safe manner by which wayward stem cells, and their progeny, can be controlled in vivo. Our approach will render the clinical application of human pluripotent stem cells increasingly possible.
- Biomaterials.Biomaterials.2013 Feb;34(6):1701-11. doi: 10.1016/j.biomaterials.2012.11.018. Epub 2012 Dec 4.
- Pluripotent stem cells, including embryonic stem (ES) and induced pluripotent stem (iPS) cells, serve as unlimited resources for cell replacement therapy and tissue engineering because such cells are capable of extensive proliferation in vitro and can give rise to lineages that represent any of the
- PMID 23218839
Japanese Journal
- Parental Alienation Syndrome Techniques of Research:Pastoral Guidance for Wayward Kids
- 國士舘大學教養論集 (58), 73-100, 2005-11
- NAID 40007283012
- The Wayward Busにおける登場人物と作品構成について
- 聖徳大学総合研究所論叢 5, 479-467, 1998-03-20
- NAID 110000487144
- 「放浪」への憧れ--SteinbeckのThe Wayward Bus再考
- 英文学論集 (37), 91-113, 1997-12
- NAID 40000207907
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