English Journal

Effect of organic matter on nitrogenase metal cofactors homeostasis in Azotobacter vinelandii under diazotrophic conditions.

Jouogo Noumsi C1,2, Pourhassan N1, Darnajoux R1, Deicke M3, Wichard T3, Burrus V2, Bellenger JP1.
Environmental microbiology reports.Environ Microbiol Rep.2015 Nov 9. doi: 10.1111/1758-2229.12353. [Epub ahead of print]
Biological nitrogen fixation can be catalyzed by three isozymes of nitrogenase: Mo-nitrogenase, V-nitrogenase and Fe-nitrogenase. The activity of these isozymes strongly depends on their metal cofactors, Mo, V and Fe, and their bioavailability in ecosystems. Here, we show how metal bioavailability c
PMID 26549632

Rees JA1,2, Bjornsson R1,3, Schlesier J4, Sippel D4, Einsle O4, DeBeer S5,6.
Angewandte Chemie (International ed. in English).Angew Chem Int Ed Engl.2015 Nov 2;54(45):13249-52. doi: 10.1002/anie.201505930. Epub 2015 Sep 17.
The first direct evidence is provided for the presence of an interstitial carbide in the FeV cofactor of Azotobacter vinelandii vanadium nitrogenase. As for our identification of the central carbide in the FeMo cofactor, we employed Fe Kβ valence-to-core X-ray emission spectroscopy and densit
PMID 26376620

Hypothetical Protein Avin_16040 as the S-Layer Protein of Azotobacter vinelandii and Its Involvement in Plant Root Surface Attachment.

Liew PW1, Jong BC2, Najimudin N3.
Applied and environmental microbiology.Appl Environ Microbiol.2015 Nov 1;81(21):7484-95. doi: 10.1128/AEM.02081-15. Epub 2015 Aug 14.
A proteomic analysis of a soil-dwelling, plant growth-promoting Azotobacter vinelandii strain showed the presence of a protein encoded by the hypothetical Avin_16040 gene when the bacterial cells were attached to the Oryza sativa root surface. An Avin_16040 deletion mutant demonstrated reduced cellu
PMID 26276116