トランスファクター

English Journal

Curcumin inhibits agent-induced human neutrophil functions in vitro and lipopolysaccharide-induced neutrophilic infiltration in vivo.

Antoine F, Simard JC, Girard D.Author information Laboratoire de recherche en inflammation et physiologie des granulocytes, Université du Québec, INRS-Institut-Armand-Frappier, Laval, Quebec Canada.AbstractCurcumin, extracted from the rhizome of Curcuma longa, is known to possess anti-inflammatory activities. Despite the fact that neutrophils are key player cells in inflammation, the role of curcumin on neutrophil cell biology is not well documented and, in particular, how curcumin can alter primed neutrophils is unknown. In addition, the effect of curcumin on agent-induced neutrophilic inflammation is not well documented. Here, we demonstrated that curcumin inhibited formyl-methionyl-leucyl-phenylalanine (fMLP)- or lipopolysaccharide (LPS)-induced suppression of human neutrophil apoptosis. In addition, we found that curcumin reversed the ability of phorbol myristate acetate (PMA) to induce reactive oxygen species as assessed by flow cytometry using the CM-H2DCF-DA probe. Using an antibody array approach, curcumin was found to inhibit LPS-induced cytokine production, including MIP-1α, MIP-1β, IL-6, IL-8 (CXCL-8) and GRO-α. The inhibitory effect of curcumin on IL-8 production was confirmed by ELISA. Using both an electrophoretic mobility shift assay and a TransFactor p50 NF-κB ELISA, we demonstrated that curcumin inhibited LPS-induced NF-κB activation. In vivo, using the murine air pouch model of acute inflammation, we demonstrated that intraperitoneal administration of curcumin inhibited LPS-induced neutrophilic infiltration in vivo. As assessed by a murine antibody array approach, curcumin was found to decrease the local production of several cytokines/chemokines induced by LPS, including, but not limit to, MIP-1α and MIP-1β. We conclude that curcumin possesses potent modulatory activities on primed or agent-induced human neutrophils in vitro and that it possesses important anti-inflammatory activities in vivo by inhibiting LPS-induced neutrophilic inflammation.
International immunopharmacology.Int Immunopharmacol.2013 Dec;17(4):1101-7. doi: 10.1016/j.intimp.2013.09.024. Epub 2013 Oct 21.
Curcumin, extracted from the rhizome of Curcuma longa, is known to possess anti-inflammatory activities. Despite the fact that neutrophils are key player cells in inflammation, the role of curcumin on neutrophil cell biology is not well documented and, in particular, how curcumin can alter primed ne
PMID 24157330

Nickel ion inhibits nuclear factor-kappa B activity in human oral squamous cell carcinoma.

Shionome T, Endo S, Omagari D, Asano M, Toyoma H, Ishigami T, Komiyama K.Author information Department of Partial Denture Prosthodontics, Nihon University School of Dentistry, Tokyo, Japan.AbstractBACKGROUND: The spontaneous IL-8 secretion observed in OSCC is partially dependent on the disregulated activity of transcription factor NF-κB. Nickel compounds are well established human carcinogens, however, little is known about the influence of nickel on the spontaneous secretion of IL-8 in oral squamous cell carcinoma (OSCC) cells. The aim of the present study was to investigate whether Ni(2+) ions can influence on IL-8 secretion by OSCC.
PloS one.PLoS One.2013 Jul 3;8(7):e68257. doi: 10.1371/journal.pone.0068257. Print 2013.
BACKGROUND: The spontaneous IL-8 secretion observed in OSCC is partially dependent on the disregulated activity of transcription factor NF-κB. Nickel compounds are well established human carcinogens, however, little is known about the influence of nickel on the spontaneous secretion of IL-8 in oral
PMID 23844176

[siRNA-induced down-regulation of Livin expression increases spontaneous apoptosis in K562 cell line].

Lü J, Chen ZC, Li QB, Shao J, You Y, Zou P.Author information Department of Hematology, Linyi Cancer Hospital, Shandong Province, China.AbstractThis study was aimed to observe the effects of siRNA on Livin expression and function in K562 cells. Livin siRNA were designed and synthesized, then were transfected into K562 cells by using AMAXA nucle transfactor. Expressions of Livin mRNA and protein in transfected K562 cells was detected by RT-PCR and Western blot respectively. Non-transfected cells were used as control. The enhanced green fluorescent protein plasmid was used as positive control and the transfection efficiency was detected by flow cytometry. Cell apoptosis was measured by flow cytometry with Annexin V-FITC/PI double staining. The results showed that the transfection efficiency of electroporation method was about 50. The synthesized siRNA inhibited livin expression at both mRNA and protein levels. The rate of K562 cell apoptosis increased from (9.63 ± 0.89) in control group to (12.07 ± 1.39) and (27.41 ± 2.30) at 24 h and 48 h after transfection, respectively (P < 0.05). It is concluded that the siRNA can inhibit anti-apoptosis of livin gene via down-regulating livin gene expression, which may provide the new method for anti-leukemia study.
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology.Zhongguo Shi Yan Xue Ye Xue Za Zhi.2012 Apr;20(2):258-61.
This study was aimed to observe the effects of siRNA on Livin expression and function in K562 cells. Livin siRNA were designed and synthesized, then were transfected into K562 cells by using AMAXA nucle transfactor. Expressions of Livin mRNA and protein in transfected K562 cells was detected by RT-P
PMID 22541077

Japanese Journal

Nrf2-DEPENDENT GENE EXPRESSIONS : A MOLECULAR TOXICOLOGICAL ASPECT

NUMAZAWA Satoshi,YOSHIDA Takemi
Journal of toxicological sciences 29(2), 81-89, 2004-05-28
… Although NF-E2 related factor 2 (Nrf2) was found to be a transcriptional regulator that controls an expression of the β-globin gene, the notion is now widely accepted that this transfactor serves as a master regulator for the gene expression of a battery of proteins acting on anti-oxidative stress and detoxification of electrophiles. …
NAID 110001812860