English Journal

Enzymatic Synthesis of Modified Oligonucleotides by PEAR Using Phusion and KOD DNA Polymerases.

Wang X1, Zhang J, Li Y, Chen G, Wang X.
Nucleic acid therapeutics.Nucleic Acid Ther.2014 Dec 17. [Epub ahead of print]
Antisense synthetic oligonucleotides have been developed as potential gene-targeted therapeutics. We previously reported polymerase-endonuclease amplification reaction (PEAR) for amplification of natural and 5'-O-(1-thiotriphosphate) (S)-modified oligonucleotides. Here, we extended the PEAR techniqu
PMID 25517220

Ectonucleotide pyrophosphatase/phosphodiesterase activity in Neuro-2a neuroblastoma cells: changes in expression associated with neuronal differentiation.

Gómez-Villafuertes R1, Pintor J, Miras-Portugal MT, Gualix J.
Journal of neurochemistry.J Neurochem.2014 Nov;131(3):290-302. doi: 10.1111/jnc.12794. Epub 2014 Jul 10.
Neuro-2a (N2a) neuroblastoma cells display an ectoenzymatic hydrolytic activity capable of degrading diadenosine polyphosphates. The Apn A-cleaving activity has been analysed with the use of the fluorogenic compound BODIPY FL guanosine 5'-O-(3-thiotriphosphate) thioester. Hydrolysis of this dinucleo
PMID 24947519

Design, synthesis, and biological evaluation of (3R)-1,2,3,4-tetrahydro-7-hydroxy-N-[(1S)-1-[[(3R,4R)-4-(3-hydroxyphenyl)-3,4-dimethyl-1-piperidinyl]methyl]-2-methylpropyl]-3-isoquinolinecarboxamide (JDTic) analogues: in vitro pharmacology and ADME profile.

Kormos CM1, Gichinga MG, Maitra R, Runyon SP, Thomas JB, Brieaddy LE, Mascarella SW, Navarro HA, Carroll FI.
Journal of medicinal chemistry.J Med Chem.2014 Sep 11;57(17):7367-81. doi: 10.1021/jm5008177. Epub 2014 Aug 25.
JDTic analogues 4-15 which have the hydroxyl groups replaced with other groups were synthesized and their in vitro efficacy at the μ, δ, and κ opioid receptors determined and compared to JDTic using [(35)S]GTPγS assays. Compounds 4, 5, 6, 13, 14, and 15 had Ke = 0.024, 0.01, 0.039, 0.02, 0.11, a
PMID 25133923

Japanese Journal

Contribution of Active and Inactive States of the Human 5-HT_<4d> Receptor to the Functional Activities of 5-HT_4-Receptor Agonists

Mikami Tadayoshi,Sugimoto Hiromi,Naganeo Rie [他],OHMI Takashi,SAITO Toshiyuki,EDA Hiroyuki
Journal of pharmacological sciences 107(3), 251-259, 2008-07-20
… Additionally, the number of [3H]5-HT binding sites decreased in the presence of guanosine-5'-O-(3-thiotriphosphate) (GTPγS), but the number of [3H]GR113,808 sites did not change. …
NAID 10024321238

Roles of the HSP70-Subunit in a Eukaryotic Multi-Site-Specific Endonuclease, Endo. SceI : Autophosphorylation and Heat Stability

, ,
Bioscience, biotechnology, and biochemistry 68(12), 2557-2564, 2004-12-23
… ATP, adenosine 5′-<I>O</I>-(3-thiotriphosphate) (ATP-γ-S), and ADP not only protected the endonucleolytic activity against heat inactivation in the presence of Ca<SUP>2+</SUP> …
NAID 130000030886

Melatonin Binding Sites in the Brain of European Sea Bass(Dicentrarchus labrax)(Endocrinology)

Bayarri Maria Jose,Garcia-Allegue Rosa,Munoz-Cueto Jose Antonio [他],Madrid Juan Antonio,Tabata Mitsuo,Sanchez-Vazquez F. Javier,Iigo Masayuki
Zoological science 21(4), 427-434, 2004-04-25
… Characteristics, day-night changes, guanosine 5'-O-(3-thiotriphosphate) (GTP-γS) modulation, and localization of melatonin binding sites in the brain of a marine teleost, European sea bass Decentrarchus labrax, were studied by radioreceptor assay using 2-[^<125>I]iodomelatonin as a radioligand. …
NAID 110006282414