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1. ダウン症候群の一過性骨髄増殖性疾患 transient myeloproliferative disorder of down syndrome
2. 急性リンパ芽球性白血病における微小残存病変の検出 detection of minimal residual disease in acute lymphoblastic leukemia
3. 急性骨髄性白血病における寛解基準および残存病変のモニタリング remission criteria in acute myeloid leukemia and monitoring for residual disease
4. 多発性骨髄腫の病理生物学 pathobiology of multiple myeloma
5. 急性骨髄性白血病の病因 pathogenesis of acute myeloid leukemia

English Journal

Multilayered molecular profiling supported the monoclonal origin of metastatic renal cell carcinoma.

Huang Y1, Gao S, Wu S, Song P, Sun X, Hu X, Zhang S, Yu Y, Zhu J, Li C, Qin Z, Xie L, Yao Q, Tang A, Li Z, Guo G, Wan S, Dong P, Sun L, Li W, Wang D, Gui Y, Yang H, Zhou F, Zhang X, Cai Z.Author information 1Shenzhen Second People's Hospital, the First Affiliated Hospital of Shenzhen University, Shenzhen, China; National-Regional Key Technology Engineering Laboratory for Clinical Application of Cancer Genomics, Shenzhen Key Laboratory of Genitourinary Tumor, Shenzhen, China.AbstractPrimary renal cell carcinomas (pRCCs) have a high degree of intratumoral heterogeneity and are composed of multiple distinct subclones. However, it remains largely unknown that whether metastatic renal cell carcinomas (mRCCs) also have startling intratumoral heterogeneity or whether development of mRCCs is due to early dissemination or late diagnosis. To decipher the evolution of mRCC, we analyzed the multilayered molecular profiles of pRCC, local invasion of the vena cava (IVC), and distant metastasis to the brain (MB) from the same patient using whole-genome sequencing, whole-exome sequencing, DNA methylome profiling, and transcriptome sequencing. We found that mRCC had a lower degree of heterogeneity than pRCC and was likely to result from recent clonal expansion of a rare, advantageous subclone. Consequently, some key pathways that are targeted by clinically available drugs showed distinct expression patterns between pRCC and mRCC. From the genetic distances between different tumor subclones, we estimated that the progeny subclone giving rise to distant metastasis took over half a decade to acquire the full potential of metastasis since the birth of the subclone that evolved into IVC. Our evidence supported that mRCC was monoclonal and distant metastasis occurred late during renal cancer progression. Thus, there was a broad window for early detection of circulating tumor cells and future targeted treatments for patients with mRCCs should rely on the molecular profiles of metastases.
International journal of cancer. Journal international du cancer.Int J Cancer.2014 Jul 1;135(1):78-87. doi: 10.1002/ijc.28654. Epub 2013 Dec 21.
Primary renal cell carcinomas (pRCCs) have a high degree of intratumoral heterogeneity and are composed of multiple distinct subclones. However, it remains largely unknown that whether metastatic renal cell carcinomas (mRCCs) also have startling intratumoral heterogeneity or whether development of m
PMID 24310851

Osteoconductive bio-based meshes based on Poly(hydroxybutyrate-co-hydroxyvalerate) and poly(butylene adipate-co-terephthalate) blends.

Nar M1, Staufenberg G1, Yang B1, Robertson L2, Patel RH3, Varanasi VG4, D'Souza NA5.Author information 1Department of Material Science and Engineering, University of North Texas, 1155 Union Circle #305310, Denton, TX 76203-5017, United States.2Department of Fibers, College of Visual Arts and Design, 1155 Union Circle #305100, Denton, TX 76203-5017, United States.3Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX 75246, United States.4Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, TX 75246, United States. Electronic address: vvaranasi@bcd.tamhsc.edu.5Department of Material Science and Engineering, University of North Texas, 1155 Union Circle #305310, Denton, TX 76203-5017, United States; Department of Mechanical and Energy Engineering, University of North Texas, 1155 Union Circle #311098, Denton, TX 76203-5017, United States. Electronic address: nandika.dsouza@unt.edu.AbstractPoly(butylene adipate-co-terephthalate) (PBAT) and Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) are biopolymers that have the potential to be used in applications of bone healing. In this study, it is hypothesized that the polymer blend has the combined strength and osteoconductivity to support osteoblast collagen formation. PBAT (PBAT 100), and a blend with 20% PHBV (PBAT 80) were extruded in the form of fibers and then knitted in the form of mesh. These were tested in the warp as well as weft direction for the tensile properties; these showed that the weft direction had higher performance than the warp. The individual fibers were kept in phosphate buffered saline (PBS) over the period of 8weeks and were tested for the storage and loss modulus using a dynamic mechanical analyser (DMA). The results indicated that mechanical relaxation strength showed a decrease and then an increase. In vitro osteoconductivity studies were done by using differentiating osteoblasts (MC3T3-E1 subclone 4 cells). Environmental Scanning Electron Microscopy (ESEM) showed that pre-soaking the samples in α-MEM for two weeks resulted in cell attachment and growth. X-ray diffraction (XRD) was used to determine the change in structure of polymers due to in vitro degradation for two weeks. Raman spectroscopy showed that all scaffolds supported the formation of a collagenous network over the scaffold surfaces. For a combination of knittable manufacturing, mechanical performance and osteoconductivity, blends offer an effective route.
Materials science & engineering. C, Materials for biological applications.Mater Sci Eng C Mater Biol Appl.2014 May 1;38:315-24. doi: 10.1016/j.msec.2014.01.047. Epub 2014 Feb 6.
Poly(butylene adipate-co-terephthalate) (PBAT) and Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) are biopolymers that have the potential to be used in applications of bone healing. In this study, it is hypothesized that the polymer blend has the combined strength and osteoconductivity to support o
PMID 24656384

Deconvolving tumor purity and ploidy by integrating copy number alterations and loss of heterozygosity.

Li Y1, Xie X.Author information 1Department of Computer Science, Institute for Genomics and Bioinformatics and Center for Machine Learning and Intelligent Systems, University of California, Irvine, CA 92697, USA.AbstractMOTIVATION: Next-generation sequencing (NGS) has revolutionized the study of cancer genomes. However, the reads obtained from NGS of tumor samples often consist of a mixture of normal and tumor cells, which themselves can be of multiple clonal types. A prominent problem in the analysis of cancer genome sequencing data is deconvolving the mixture to identify the reads associated with tumor cells or a particular subclone of tumor cells. Solving the problem is, however, challenging because of the so-called 'identifiability problem', where different combinations of tumor purity and ploidy often explain the sequencing data equally well.
Bioinformatics (Oxford, England).Bioinformatics.2014 Apr 21. [Epub ahead of print]
MOTIVATION: Next-generation sequencing (NGS) has revolutionized the study of cancer genomes. However, the reads obtained from NGS of tumor samples often consist of a mixture of normal and tumor cells, which themselves can be of multiple clonal types. A prominent problem in the analysis of cancer gen
PMID 24695406

Japanese Journal

Combined Cytolytic Effects of a Vaccinia Virus Encoding a Single Chain Trimer of MHC-I with a Tax-Epitope and Tax-Specific CTLs on HTLV-I-Infected Cells in a Rat Model

Ohashi Takashi,Nakamura Takafumi,Kidokoro Minoru,Zhang Xianfeng,Shida Hisatoshi
Biomed Research International 2014, 902478, 2014
… Combined treatment withm8 Delta/RT1AlSCTax180L and 4O1/C8 increased the cytolysis of FPM1V.EFGFP/8R cells, a CTL-resistant subclone of FPM1, compared with that using 4O1/C8 and m8. …
NAID 120005434503

Combination of guanine arabinoside and Bcl-2 inhibitor YC137 overcome the cytarabine resistance in HL-60 leukemia cell line.

Nishi Rie,Yamauchi Takahiro,Negoro Eiju,Takemura Haruyuki,Ueda Takanori
Cancer Science 104, 502-507, 2013-04
… The established subclone HL-60/ara-C60 was 60-fold more ara-C-resistant than the parental HL-60 cells. …
NAID 120005254373

Establishment of a highly migratory subclone reveals that CD133 contributes to migration and invasion through epithelial-mesenchymal transition in pancreatic cancer