UpToDate Contents

全文を閲覧するには購読必要です。 To read the full text you will need to subscribe.

English Journal

Mitchell SF1, Lorsch JR2.
Methods in enzymology.Methods Enzymol.2015;559:111-25. doi: 10.1016/bs.mie.2014.11.002. Epub 2015 May 16.
Isolation of highly purified recombinant protein is essential for a wide range of biochemical and biophysical assays. Affinity purification in which a tag is fused to the desired protein and then specifically bound to an affinity column is a widely used method for obtaining protein of high purity. M
PMID 26096506

An optimized intein-mediated protein ligation approach for the efficient cyclization of cysteine-rich proteins.

Tarasava K1, Freisinger E2.
Protein engineering, design & selection : PEDS.Protein Eng Des Sel.2014 Dec;27(12):481-8. doi: 10.1093/protein/gzu048. Epub 2014 Oct 21.
Head-to-tail backbone cyclization of proteins is a widely used approach for the improvement of protein stability. One way to obtain cyclic proteins via recombinant expression makes use of engineered Intein tags, which are self-cleaving protein domains. In this approach, pH-induced self-cleavage of t
PMID 25335928

Bacterial inclusion bodies as potential synthetic devices for pathogen recognition and a therapeutic substance release.

Talafová K1, Hrabárová E, Chorvát D, Nahálka J.
Microbial cell factories.Microb Cell Fact.2013 Feb 7;12:16. doi: 10.1186/1475-2859-12-16.
BACKGROUND: Adhesins of pathogens recognise the glycans on the host cell and mediate adherence. They are also crucial for determining the tissue preferences of pathogens. Currently, glyco-nanomaterials provide potential tool for antimicrobial therapy. We demonstrate that properly glyco-tailored incl
PMID 23391325