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Erythrocyte rosetting or E-rosetting is a phenomenon seen through a microscope where red blood cells (erythrocytes) are arranged around a central cell to form a cluster that looks like a flower. The red blood cells surrounding the cell form the petal, while the central cell forms the stigma of the flower shape. This formation occurs due to an immunological reaction between an epitope on the central cells surface and a receptor or antibody on a red blood cell.[1] The presence of E-rosetting can be used as a test for T cells although more modern tests such as immunohistochemistry are available. May be caused by Plasmodium in malaria.
Contents
- 1 Rosetting techniques
- 1.1 Rosette test for Rh factor
- 1.2 E-rosetting
- 1.3 EA-rosetting
- 1.4 EAC-rosetting
- 2 References
Rosetting techniques[edit]
Three types of rosette techniques have been developed and used experimentally.[citation needed]
Rosette test for Rh factor[edit]
The Rosette test is performed on postpartum maternal blood to estimate the volume of fetal-maternal hemorrhage in case of an Rh negative mother and an Rh positive child. This estimate, in turn, also estimates the required amount of Rho(D) immune globulin to administer.[2] In this test, a sample of maternal blood is incubated with Rho(D) immune globulin, which will bind to any fetal Rh positive red blood cells, if present.[3] Upon addition of enzyme-treated cDE indicator cells, the presence of Rh positive fetal blood causes rosetting, which can be seen by light microscopy.[3] The test is recommended for Rh negative mothers within 72 hours of giving birth to an Rh-positive infant.[2] In a positive test, it is recommended that a Kleihauer–Betke test should be performed to confirm and quantify any positive rosette tests.[2]
E-rosetting[edit]
E-rosetting is used in the identification of T cells where a T cells CD2 surface protein is bound to a sugar based LFA-3 homologue on the surface of a sheep red blood cell.[1] Because the LFA-3 homologue is only present on the surface of sheep red blood cells other species red blood cells can not be used in this type of rosetting.[citation needed]
EA-rosetting[edit]
Erythrocyte antibody rosetting (EA-rosetting), occurs when an antibody molecule that is specific for an epitope on another cell is embedded in the membrane of a red blood cell and then reacted against a cell carrying the epitope that the antibody is specific for.[citation needed]
EAC-rosetting[edit]
Erythrocyte antibody complement rosetting (EAC-rosetting), occurs when antibody in the presence of complement is bound to the surface of a red blood cell. The complement binds to the tail region (Fc region) of the antibody. Finally T-cells with a complement receptor are added and the T-cells bind to the complement on the antibody completing the rosette.[citation needed]
References[edit]
- ^ a b Ocklind, G (18 January 1988). "Activation of Human T Lymphocytes through CD3 and CD2 (T11) with Anti-CD3-Coupled Sheep Erythrocytes". Scandinavian Journal of Immunology 27: 609–613. doi:10.1111/j.1365-3083.1988.tb02389.x.
- ^ a b c FETAL Maternal hemorrhage SCREEN (007-0155) at The University of Texas Medical Branch at Galveston. Reviewed/Revised 6/27/10 by T. Moore, MT(ASCP)SBB
- ^ a b Rosette test at the TraQ Program of the BC Provincial Blood Coordinating Office. A program of the Provincial Health Services Authority. January 2005
Medical test: Serology, reference range: Clinical biochemistry blood tests (including BMP, CMP) (CPT 82000–84999)
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Fluid/electrolytes |
- electrolytes
- renal function, BUN-to-creatinine ratio
- Ca
- derived values: Plasma osmolality
- Serum osmolal gap
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Acid-base |
- Arterial blood gas
- Base excess
- Anion gap
- CO2 content
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Nutrition |
- Iron tests: Transferrin saturation = Serum iron / Total iron-binding capacity; Ferritin
- Transferrin
- Transferrin receptor
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Endocrine |
- ACTH stimulation test
- Thyroid function tests
- Blood sugar: Glucose test
- C-peptide
- Fructosamine
- Glycated hemoglobin
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Metabolic |
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Cardiovascular |
- Cardiac marker: Troponin test
- CPK-MB test
- LDH
- Myoglobin
- Glycogen phosphorylase isoenzyme BB
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Digestive |
- Liver function tests: protein tests
- Human serum albumin
- Serum total protein
- ALP
- transaminases
- Bilirubin
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noco/acba/cong/tumr, sysi/epon, urte
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proc/itvp, drug (G4B), blte, urte
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noco (d)/cong/tumr, sysi/epon
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proc, drug (A10/H1/H2/H3/H5)
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noco/cong/tumr, sysi/epon, injr
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proc, drug (C1A/1B/1C/1D), blte
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anat (t, g, p)/phys/devp/enzy
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noco/cong/tumr, sysi/epon
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proc, drug (A2A/2B/3/4/5/6/7/14/16), blte
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UpToDate Contents
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English Journal
- More than just immune evasion: Hijacking complement by Plasmodium falciparum.
- Schmidt CQ1, Kennedy AT2, Tham WH3.
- Molecular immunology.Mol Immunol.2015 Sep;67(1):71-84. doi: 10.1016/j.molimm.2015.03.006. Epub 2015 Mar 26.
- Malaria remains one of the world's deadliest diseases. Plasmodium falciparum is responsible for the most severe and lethal form of human malaria. P. falciparum's life cycle involves two obligate hosts: human and mosquito. From initial entry into these hosts, malaria parasites face the onslaught of t
- PMID 25816986
- P-selectin promotes neutrophil extracellular trap formation in mice.
- Etulain J1, Martinod K2, Wong SL3, Cifuni SM4, Schattner M5, Wagner DD6.
- Blood.Blood.2015 Jul 9;126(2):242-6. doi: 10.1182/blood-2015-01-624023. Epub 2015 May 15.
- Neutrophil extracellular traps (NETs) can be released in the vasculature. In addition to trapping microbes, they promote inflammatory and thrombotic diseases. Considering that P-selectin induces prothrombotic and proinflammatory signaling, we studied the role of this selectin in NET formation. NET f
- PMID 25979951
- α2-Macroglobulin Can Crosslink Multiple Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) Molecules and May Facilitate Adhesion of Parasitized Erythrocytes.
- Stevenson L1, Laursen E1, Cowan GJ2, Bandoh B3, Barfod L1, Cavanagh DR2, Andersen GR4, Hviid L1.
- PLoS pathogens.PLoS Pathog.2015 Jul 2;11(7):e1005022. doi: 10.1371/journal.ppat.1005022. eCollection 2015.
- Rosetting, the adhesion of Plasmodium falciparum-infected erythrocytes to uninfected erythrocytes, involves clonal variants of the parasite protein P. falciparum erythrocyte membrane protein 1 (PfEMP1) and soluble serum factors. While rosetting is a well-known phenotypic marker of parasites associat
- PMID 26134405
Japanese Journal
- First report of Candidatus Phytoplasma asteris associated with yellowing and rosetting of Japanese spurge in Korea
- BACK Chang-Gi,CHUNG Heewon,JUNG Hee-Young
- Journal of general plant pathology : JGPP 76(5), 355-357, 2010-10-01
- NAID 10029720110
- キク組織培養苗の冷蔵処理による定植後のロゼット化防止
- 浅見 逸夫,山田 眞人
- 愛知県農業総合試験場研究報告 (41), 21-27, 2009-12
- 白花一輪キク品種の「白粋」、「神馬」、スプレーギク品種の「ホワイトインパクト」を用いて、培養苗への冷蔵処理が定植後の生育に及ぼす影響を調査した。1.25℃設定の培養室において、培養棚の蛍光灯を全て点灯した場合、培養容器内の温度は最高30.0℃、培養苗の葉温は最高29.4℃、培地温度は最高27.9℃を記録した。2.培養苗を照明条件下で5℃冷蔵すると、8週間冷蔵しても茎長が約2.5mmしか伸長しなかっ …
- NAID 40017054142
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- 今村 仁,須藤 憲一,池田 広
- 園芸学研究 8(1), 41-46, 2009-01-15
- トルコギキョウの種子登熟期に未熟な朔果(未熟果)に対する温度処理を行い,登熟時の温度条件が高温下で播種された実生の抽だいに及ぼす影響を調査した.登熟時の高温(38/28℃:昼/夜)は,涼温(23/18℃)と比較して抽だい率を低下させた.また,涼温条件と,暖地の雨よけハウスにおける夏から秋にかけての温度条件で登熟させた種子の抽だい率はほぼ同じになった.受粉後3週間の'彩の桜'の未熟果を花梗をつけて植 …
- NAID 110007043558
Related Links
- Erythrocyte rosetting or E-rosetting is a phenomenon seen through a microscope where red blood cells (erythrocytes) are arranged around a central cell to form a cluster that looks like a flower. The red blood cells surrounding the cell form the ...
- Department of Infectious Diseases, Karolinska Institutet, Huddinge Hospital, Sweden. Spontaneous binding of uninfected erythrocytes to Plasmodium falciparum-infected erythrocytes (rosetting) has been suggested to have a critical role in the ...
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