リボプローブ、RNAプローブ
- 関
- RNA probe
Wikipedia preview
出典(authority):フリー百科事典『ウィキペディア(Wikipedia)』「2015/07/04 12:00:08」(JST)
[Wiki en表示]
Riboprobes are RNA probes that can be produced by in vitro transcription of cloned DNA inserted in a suitable plasmid downstream of a viral promoter. Some bacterial viruses code for their own RNA polymerases, which are highly specific for the viral promoters. Using these enzymes, labeled NTPs, and inserts inserted in both forward and reverse orientations, both sense and antisense riboprobes can be generated from a cloned gene. Riboprobes are utilized during in situ hybridization.
References
Baynes, John W.; Marek H. Dominiczak (2005). Medical Biochemistry 2nd. Edition. Elsevier Mosby. p. 477. ISBN 0-7234-3341-0.
UpToDate Contents
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English Journal
- Gene Expression Analysis of CCN Proteins: Whole-Mount In Situ Hybridization of Ccn2 in Developing Calcified Tissues.
- Yamaai T1, Takigawa M2.
- Methods in molecular biology (Clifton, N.J.).Methods Mol Biol.2017;1489:11-19.
- A procedure for whole-mount in situ hybridization developed for detecting gene expression of Ccn2 in developing calcified tissues of mouse embryos is presented. In this method, embryos are hybridized with Dig-labeled riboprobes, and the riboprobes are detected by use of the alkaline-phosphatase reac
- PMID 27734361
- An Optimized Small Tissue Handling System for Immunohistochemistry and In Situ Hybridization.
- Anthony G1, Lee JA1.
- PloS one.PLoS One.2016 Aug 4;11(8):e0159991. doi: 10.1371/journal.pone.0159991. eCollection 2016.
- Recent development in 3D printing technology has opened an exciting possibility for manufacturing 3D devices on one's desktop. We used 3D modeling programs to design 3D models of a tissue-handling system and these models were "printed" in a stereolithography (SLA) 3D printer to create precision hist
- PMID 27489962
- Distribution pattern of bovine viral diarrhoea virus type 1 genome in lymphoid tissues of experimentally infected sheep.
- Karikalan M1, Rajukumar K2, Mishra N1, Kumar M1, Kalaiyarasu S1, Rajesh K1, Gavade V1, Behera SP1, Dubey SC1.
- Veterinary research communications.Vet Res Commun.2016 Jun;40(2):55-61. doi: 10.1007/s11259-016-9654-9. Epub 2016 Mar 21.
- In this study, cellular localization and the distribution pattern of BVDV genome in lymphoid tissues during the course of experimental acute BVDV-1 infection of sheep was investigated. Tonsils, mesenteric lymph nodes (MLN) and spleen were collected on 3, 6, 9, 12 and 15 days post infection (dpi) fr
- PMID 26996785
Japanese Journal
- Detection of miRNA in Cell Cultures by Using Microchip Electrophoresis with a Fluorescence-Labeled Riboprobe
- ホールマウントin situハイブリダイゼーション法とその応用(B.遺伝子発現の解析技術,見るバイオサイエンス-基礎から最先端技術まで)
- ホールマウントin situハイブリダイゼーション(B.in situハイブリダイゼーション法)
Related Links
- Riboprobe ® Systems* 製品説明 本システムは、RI標識の1本鎖RNAプローブの調製、あるいはug単位のRNA転写産物をin vitroで合成するために使用します。テンプレートDNAの転写反応に必要な全ての試薬が含まれているだけでなく ...
- Riboprobe ® Systemのバッファー類は、単品でご利用いただけます。トランスクリプションによる品質保証が行われているため、良好なin vitro転写反応を行えます。
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- 英
- RNA probe、riboprobe
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- リボプローブ
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- 英
- riboprobe
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- RNAプローブ
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- riboprobe