English Journal

Enzymological description of multitemplate PCR-Shrinking amplification bias by optimizing the polymerase-template ratio.

Ingr M1, Dostál J2, Majerová T3.
Journal of theoretical biology.J Theor Biol.2015 Oct 7;382:178-86. doi: 10.1016/j.jtbi.2015.06.048. Epub 2015 Jul 9.
Multitemplate polymerase chain reaction (PCR) is used for preparative and analytical applications in diagnostics and research. Classical PCR and qPCR are two basic setups with many possible experimental modifications. Classical PCR is a method of choice to obtain enough material for subsequent sophi
PMID 26164060

The effect of the carbohydrate binding module on substrate degradation by the human chitotriosidase.

Stockinger LW1, Eide KB1, Dybvik AI2, Sletta H3, Vårum KM2, Eijsink VG1, Tøndervik A3, Sørlie M4.
Biochimica et biophysica acta.Biochim Biophys Acta.2015 Oct;1854(10 Pt A):1494-501. doi: 10.1016/j.bbapap.2015.06.008. Epub 2015 Jun 24.
Human chitotriosidase (HCHT) is one of two active glycoside hydrolase family 18 chitinases produced by humans. The enzyme is associated with several diseases and is thought to play a role in the anti-parasite responses of the innate immune system. HCHT occurs in two isoforms, one 50kDa (HCHT50) and
PMID 26116146

Sumathy S1, Satyanarayana SV2.
Journal of theoretical biology.J Theor Biol.2015 Sep 7;380:48-52. doi: 10.1016/j.jtbi.2015.04.029. Epub 2015 May 2.
Cytoplasmic dynein exhibits a directional processive movement on microtubule filaments and is known to move in steps of varying length based on the number of ATP molecules bound to it and the load that it carries. It is experimentally observed that dynein takes occasional backward steps and the freq
PMID 25944174

Japanese Journal

Mizuno Hiroaki,Watanabe Naoki
BIOPHYSICS 8(0), 95-102, 2012
… Recently, we have demonstrated that a mammalian formin mDia1 rotates along the long-pitch helix of F-actin during processive elongation (helical rotation) by single-molecule fluorescence polarization. … We have also shown processive depolymerization of mDia1-bound F-actin during which helical rotation was visualized. …
NAID 130002061590

水野裕昭,渡邊直樹
生物物理 51(5), 218-221, 2011-09-25
… Here, we show the rotational movement of a mammalian formin mDia1 along the long-pitch helix of F-actin during processive elongation and depolymerization. … From these properties, we postulate that helical rotation of formins may regulate actin assembly and filament stabilization during processive F-actin elongation. …
NAID 10029482460