English Journal
- Brilliant violet fluorochromes in simultaneous multicolor flow cytometry-fluorescence in situ hybridization measurement of monocyte subsets and telomere length in heart failure.
- Roura S1,2, Fernández MA3, Elchinova E4,5, Teubel I3, Requena G3, Cabanes R4, Lupón J4,5, Bayes-Genis A1,4,5.
- Laboratory investigation; a journal of technical methods and pathology.Lab Invest.2016 Nov;96(11):1223-1230. doi: 10.1038/labinvest.2016.100. Epub 2016 Sep 12.
- PMID 27617397
- Genomic Signatures of North American Soybean Improvement Inform Diversity Enrichment Strategies and Clarify the Impact of Hybridization.
- Vaughn JN1, Li Z2.
- G3 (Bethesda, Md.).G3 (Bethesda).2016 Sep 8;6(9):2693-705. doi: 10.1534/g3.116.029215.
- PMID 27402364
- An alternative method for processing northern blots after capillary transfer.
- Nilsen TW.
- Cold Spring Harbor protocols.Cold Spring Harb Protoc.2015 Mar 2;2015(3):314-8. doi: 10.1101/pdb.prot081026.
- Different laboratories use different methods for the prehybridization, hybridization, and washing steps of the northern blotting procedure. In this protocol, a northern blot is pretreated with Church and Gilbert hybridization buffer to block nonspecific probe-binding sites. The immobilized RNA is th
- PMID 25734064
Japanese Journal
- Cold in-situ-hybridization of human T-cell leukemia virus type 1: Production of biotinylated probes by polymerase chain reaction and improvement of procedures
- Hasui Kazuhisa,Sato Eiichi,Sueyoshi Kazunobu,Kitajima Shinichi,Goto Masamitsu,Nakamura Takao,Matsumoto Daiten
- Dendritic cells 5, 27-35, 1995-00-00
- … DNA-RNA cISH of these probes was established, evaluating the effect of 17% polyvinyl alcohol solvent for reducing non-specific alkaline phosphatase reaction in the process of visualizing hybridized probe and suggesting the necessary prehybridization incubation with hybridization buffer containing salmon DNA for diminishing non-specific binding of probes with tissue. …
- NAID 120001392791
- 反復DNA配列のin situハイブリダイゼ-ションによるライムギB染色体のDNA構造〔英文〕
- 辻本 寿,丹羽 克昌
- The Japanese journal of genetics 67(3), 233-241, 1992-06-25
- … A probe of total DNA from a 4B plant produced signals only in the terminal C-band region after prehybridization with the 0 B plant DNA. …
- NAID 10006699293
- PRENATAL DIAGNOSIS OF DUCHENNE MUSCULAR DYSTROPHY BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS WITH pERT 87 INTRAGENOMIC DEOXYRIBONUCLEIC ACID PROBES
- KATAYAMA Susumu,YANO Tomone,TAKESHITA Naoki,ABE Yuji,USUI Akira,KUBO Harumi,MOMOSE Kazuo,UBAGAI Tsuneyuki
- 日本産科婦人科學會雜誌 43(6), 633-640, 1991-06-01
- DNAプローブと妊娠初期絨毛採取法の組合せによる Duchenne型筋ジストロフィー症の出生前診断を行つた. 遺伝性症例4家系, 孤発性症例4家系合計8家系を対象とした. 成人(50名)DNAは末梢血白血球, 胎児(8名)DNAは妊娠初期9週に絨毛採取法により採取した絨毛組織より常法により抽出した. 7μg DNAを制限酵素 (BstXI, TaqI, XmnI, BstNI) で切断して1%アガ …
- NAID 110002106129
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- prehybridization
- 関
- プレハイブリ