English Journal

Paired box 2 induces epithelial-mesenchymal transition in normal renal tubular epithelial cells of rats.

Li L, Wu Y, Yang Y.SourceDepartment of Pediatric Nephrology and Rheumatology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.
Molecular medicine reports.Mol Med Rep.2013 May;7(5):1549-54. doi: 10.3892/mmr.2013.1365. Epub 2013 Mar 12.
Epithelial‑mesenchymal transition (EMT) is considered to be a crucial stage of renal fibrosis. Previous studies have indicated that paired box 2 (PAX2) affects renal fibrosis, likely by regulating EMT. Therefore, we examined whether PAX2 directly induces normal renal tubular EMT in vitro. Recomb
PMID 23503776

MARCKS and HSP70 interactions regulate mucin secretion by human airway epithelial cells in vitro.

Fang S, Crews AL, Chen W, Park J, Yin Q, Ren XR, Adler KB.SourceNorth Carolina State Univ., College of Veterinary Medicine, 1060 William Moore Dr., Raleigh, NC 27607. kenneth_adler@ncsu.edu.
American journal of physiology. Lung cellular and molecular physiology.Am J Physiol Lung Cell Mol Physiol.2013 Apr;304(8):L511-8. doi: 10.1152/ajplung.00337.2012. Epub 2013 Feb 1.
Myristoylated alanine-rich C kinase substrate (MARCKS) protein has been recognized as a key regulatory molecule controlling mucin secretion by airway epithelial cells in vitro and in vivo. We recently showed that two intracellular chaperones, heat shock protein 70 (HSP70) and cysteine string protein
PMID 23377348

Optimization of electrotransfection conditions of mammalian cells with different biological features.

Guo H, Hao R, Wei Y, Sun D, Sun S, Zhang Z.SourceState Key Laboratory of Veterinary Etiological Biology and National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, 730046 Gansu, People's Republic of China. ghch-2004@126.com
The Journal of membrane biology.J Membr Biol.2012 Dec;245(12):789-95. doi: 10.1007/s00232-012-9480-0. Epub 2012 Jul 27.
We introduced eukaryotic expression plasmid pEGFP-N1 encoding green fluorescent protein (GFP) genes into cells with different biological features through electroporation. The effects of conditions, including voltage, capacitor flow, pulse cycle, DNA dosage and buffer, on transfection efficiency were
PMID 22836669

Japanese Journal

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Journal of Veterinary Medical Science 73(8), 1097-1100, 2011
… Posttransfection, PCV2 (ORF1)-EGFP/pSK, PCV2 (ORF3)-EGFP/pSK, PCV2 (ORF4)-EGFP/pSK and PCV2(ORF5)-EGFP/pSK showed no fluorescent signals in transfected cells, while green fluorescent signals were observed in the nuclei of PK-15 cells after PCV2 (ORF2)-EGFP/pSK transfection. …
NAID 130000677099

Culture Time-Dependent Gene Expression in Isolated Primary Cultured Rat Hepatocytes by Transfection with the Cationic Liposomal Vector TFL-3

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Biological & pharmaceutical bulletin 26(6), 880-885, 2003-06-01
… The transfection efficiency was determined at various times posttransfection with pDNA coding for chloramphenicol acetyltransferase (CAT), luciferase, or β-galactosidase. …
NAID 110003608537