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English Journal

Factors affecting efficiency of introducing foreign DNA and RNA into parthenogenetic or in vitro-fertilized porcine eggs by cytoplasmic microinjection.

Liu S1,2, Liu X3, Huang H3, Liu Q4, Su X4, Zhu P4, Li H4, Cui K4, Xie B4, Shi D4.
In vitro cellular & developmental biology. Animal.In Vitro Cell Dev Biol Anim.2016 Aug;52(7):713-22. doi: 10.1007/s11626-016-0025-1. Epub 2016 Apr 29.
Cytoplasmic microinjection (CI) of foreign gene into in vivo fertilized zygotes has emerged as a useful tool for transgenic pig production. In the current study, we investigated factors affecting transgenic efficiency and developmental potential of parthenogenetic (PA) and in vitro-fertilized (IVF)
PMID 27130683

Cortical mechanics and myosin-II abnormalities associated with post-ovulatory aging: implications for functional defects in aged eggs.

Mackenzie AC1, Kyle DD1, McGinnis LA1, Lee HJ1, Aldana N1, Robinson DN2, Evans JP3.
Molecular human reproduction.Mol Hum Reprod.2016 Jun;22(6):397-409. doi: 10.1093/molehr/gaw019. Epub 2016 Feb 26.
STUDY HYPOTHESIS: Cellular aging of the egg following ovulation, also known as post-ovulatory aging, is associated with aberrant cortical mechanics and actomyosin cytoskeleton functions.STUDY FINDING: Post-ovulatory aging is associated with dysfunction of non-muscle myosin-II, and pharmacologically
PMID 26921397

Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae).

Dos Santos MP1, Yasui GS2, Xavier PL3, de Macedo Adamov NS2, do Nascimento NF1, Fujimoto T4, Senhorini JA2, Nakaghi LS1.
Zygote (Cambridge, England).Zygote.2016 May 25:1-13. [Epub ahead of print]
The aim of this study was to describe the morphology of gametes, post-fertilization events and subsequent temperature effects on the early developmental stages of the neotropical species Astyanax altiparanae. The sperm of this species presents a typical morphology of teleost sperm with a spherical h
PMID 27220819

Effects of Exposure to Zearalenone on Porcine Oocytes and Sperm During Maturation and Fertilization In Vitro

SAMBUU Rentsenkhand,TAKAGI Mitsuhiro,NAMULA Zhao,OTOI Takeshige,SHIGA Satoshi,RODRIGUES DOS SANTOS Regiane,FINK-GREMMELS Johanna
The Journal of reproduction and development 57(4), 547-550, 2011-08-01
… However, neither increases in the rates of degeneration and DNA fragmentation of oocytes nor reductions in normal and polyspermic fertilization were observed. … however, 1000 μg/l ZEN had positive effects on normal and polyspermic fertilization rates. …
NAID 10029593519

SAKAGUCHI Yosuke,IWATA Hisataka,KUWAYAMA Takehito,MONJI Yasunori
The Journal of reproduction and development 55(6), 676-684, 2009-12-01
… Supplementation of the sperm-oocyte co-incubation medium with 5 mM GlcNAc also enhanced the rate of polyspermic fertilization. …
NAID 10026322923