English Journal

[Expression, purification and functional identification of human PSMP recombinant protein in Chinese hamster ovary cells].

Ma J1, Pei XL1, Zhang Y1, Wang Y1.
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences.Beijing Da Xue Xue Bao.2014 Oct 18;46(5):669-75.
OBJECTIVE: To construct a new human chemotactic cytokine PSMP eukaryotic expression vector to express PSMP in Chinese hamster ovary (CHO) cells and to obtain the purified recombinant PSMP protein for its functional mechanism study.METHODS: PSMP-myc/His fragment, cut from pcDNA3.1-PSMP-myc/His, was i
PMID 25331384

The preparation of HL-60 cells vaccine expressing BCG heat shock protein 70 and detection of its immunogenicity in vitro.

Li XL1, Zhao YX, Sun LR, Yang J, Xu HJ.
Human vaccines & immunotherapeutics.Hum Vaccin Immunother.2012 Oct;8(10):1376-81. doi: 10.4161/hv.21321. Epub 2012 Aug 16.
Gene-modified cell vaccines are the best way to achieve the immunotherapy for all types of acute leukemia. In this study, the recombinant eukaryotic expression vector (pDisplay-HSP70) of heat shock protein 70 (HSP70) of Bacille Calmette-Guérin (BCG) was constructed by amplifying the whole BCG HSP70
PMID 22894947

Blocking the large extracellular loop (LEL) domain of FcTetraspanin-3 could inhibit the infection of white spot syndrome virus (WSSV) in Chinese shrimp, Fenneropenaeus chinensis.

Gui L1, Wang B, Li FH, Sun YM, Luo Z, Xiang JH.
Fish & shellfish immunology.Fish Shellfish Immunol.2012 Jun;32(6):1008-15. doi: 10.1016/j.fsi.2012.02.022. Epub 2012 Mar 2.
Tetraspanins belong to the transmembrane 4 superfamily (TM(4)SF), which span the cell membrane 4 times and act as bridges or connectors. Increasing evidences have shown that tetraspanins play important role in virus infection. The large extracellular loop (LEL) of a tetraspanin is considered as a po
PMID 22406449

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