多染性赤芽球
WordNet
- (of light or other electromagnetic radiation) composed of more than one wavelength; "polychromatic light"
- having or exhibiting many colors (同)polychrome, polychromic
- a nucleated cell in bone marrow from which red blood cells develop
UpToDate Contents
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- 1. マラリアにおける貧血anemia in malaria [show details]
…vivax . In a small study of six children with chronic disease, an increased proportion of polychromatic erythroblasts in the G2 phase of division was observed . After treatment of malaria, the reticulocyte …
- 2. 赤血球産生調節regulation of erythropoiesis [show details]
… form small colonies of erythroblasts in seven days of culture. The erythroid precursor or erythroblast pool represents about one-third… Proerythroblasts are the earliest recognizable forms These cells divide and mature through basophilic, polychromatic, and orthochromatic normoblast cells to form the reticulocyte and finally to the circulating mature …
- 3. 鉄芽球性貧血の原因および病態生理causes and pathophysiology of the sideroblastic anemias [show details]
…anemias with defective heme synthesis, impaired hemoglobin production disrupts erythroblast maturation and the faulty erythroblasts are destroyed via mechanisms such as apoptosis, similar to thalassemias . …
- 4. 急性骨髄性白血病の分類classification of acute myeloid leukemia [show details]
…without AML-associated recurrent genetic abnormalities in whom erythroblasts account for >80 percent of the marrow cells. The erythroblasts are primarily at the pronormoblast stage and may have vacuolization …
- 5. サラセミアの病態生理pathophysiology of thalassemia [show details]
…somewhat soluble tetramers. They do not produce a major inclusion body burden in early- to mid-stage erythroblasts. Ineffective erythropoiesis is thus less marked in most alpha thalassemia patients (relative to …
English Journal
- An automated new technique for scoring the in vivo micronucleus assay with image analysis.
- Shibai-Ogata A, Tahara H, Yamamoto Y, Fujita M, Satoh H, Yuasa A, Hioki T, Kasahara T.Author information Safety Evaluation Center, Fujifilm Corporation, 210 Nakanuma, Minamiashigara-shi, Kanagawa 250-0193, Japan.AbstractThe mammalian erythrocyte micronucleus assay is frequently used to assess chemical-induced damage to the chromosomes or the mitotic apparatus of erythroblasts. Because quantitative analysis of micronuclei by microscopy is time consuming and laborious, several automatic scoring methodologies with image analysis have been reported. However, there have been cases in which it was difficult to examine the proportion of polychromatic erythrocytes (PCEs) among total erythrocytes as an index for bone marrow (BM) toxicity, and sample slide preparation has proven to be laborious with existing automatic methods. We developed an automatic scoring system with image analysis for the rodent erythrocyte micronucleus assay using 12-well plates employing high-content screening analyser. In our method, micronucleated PCEs (MNPCEs), PCEs and erythrocytes were identified from three kinds of images: bright field image, fluorescence image with Hoechst 33342, and fluorescence image with propidium iodide. The frequencies of MNPCEs and PCEs were subsequently calculated. A comparison of automatic and manual scoring was carried out using BM and peripheral blood (PB) obtained from mice treated with stepwise doses of mitomycin C. The scores obtained by automatic analysis corresponded to those obtained by manual scoring; the frequencies of MNPCEs in BM and PB obtained by automatic scoring were 132 and 113%, respectively, of those obtained by manual scoring, and the corresponding frequencies of PCEs were 95 and 120%, respectively. Furthermore, we performed five repeats of the examinations of mouse BM and PB treated with mitomycin C or vinblastine sulphate and showed that automatic scoring was equivalent to manual scoring in reproducibility. Meanwhile, the scoring data obtained by manual scoring tended to vary among observers. These results suggest that our automatic scoring system with image analysis is superior to manual microscopy scoring in terms of speed and objectivity, comparable in reproducibility and useful for the in vivo micronucleus assay.
- Mutagenesis.Mutagenesis.2013 Dec 16. [Epub ahead of print]
- The mammalian erythrocyte micronucleus assay is frequently used to assess chemical-induced damage to the chromosomes or the mitotic apparatus of erythroblasts. Because quantitative analysis of micronuclei by microscopy is time consuming and laborious, several automatic scoring methodologies with ima
- PMID 24342936
- Ascorbic acid and dietary polyphenol combinations protect against genotoxic damage induced in mice by endogenous nitrosation.
- Abraham SK, Khandelwal N.Author information School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: skabraham44@yahoo.co.in.AbstractWe investigated whether combinations of ascorbic acid (AA) plus dietary polyphenols can protect in vivo against genotoxic damage induced by endogenous nitrosation. A nitrosation reaction mixture consisting of methylurea (MU) plus sodium nitrite (SN), which can react to form N-nitroso-N-methylurea in the stomach, was administered orally to mice, together with AA and one of the dietary polyphenols ferulic acid (FA), gallic acid (GA), chlorogenic acid (CA), or epigallocatechin gallate (EGCG). Genotoxic damage in bone marrow cells was assessed by measuring micronucleated polychromatic erythrocytes (Mn PCEs) and metaphase chromosome aberrations. When compared to damage induced by MU plus SN alone, co-administration with AA, FA, GA, CA, or EGCG resulted in significant protective effects. Combinations of AA plus EGCG or AA plus CA showed a further protective effect. Reduction in the frequency of Mn PCEs to the control level was obtained following co-administration of a combination of AA, FA, GA, and CA with MU plus SN. A similar trend was observed for metaphase chromosome aberrations. Co-administration of AA, FA, GA, or CA with N-nitroso-N-methylurea (MNU) did not show any significant reduction in genotoxicity, indicating the absence of a protective effect against a preformed N-nitroso compound. Our work demonstrates the protective effects of the 'antinitrosating' combination of AA and dietary polyphenols FA, GA, or CA against genotoxic damage induced by an endogenously formed N-nitroso compound.
- Mutation research.Mutat Res.2013 Oct 9;757(2):167-72. doi: 10.1016/j.mrgentox.2013.08.004. Epub 2013 Aug 22.
- We investigated whether combinations of ascorbic acid (AA) plus dietary polyphenols can protect in vivo against genotoxic damage induced by endogenous nitrosation. A nitrosation reaction mixture consisting of methylurea (MU) plus sodium nitrite (SN), which can react to form N-nitroso-N-methylurea in
- PMID 23973768
- Autophagy facilitates organelle clearance during differentiation of human erythroblasts: evidence for a role for ATG4 paralogs during autophagosome maturation.
- Betin VM, Singleton BK, Parsons SF, Anstee DJ, Lane JD.Author information Cell Biology Laboratories, School of Biochemistry, University of Bristol, Bristol, UK.AbstractWholesale depletion of membrane organelles and extrusion of the nucleus are hallmarks of mammalian erythropoiesis. Using quantitative EM and fluorescence imaging we have investigated how autophagy contributes to organelle removal in an ex vivo model of human erythroid differentiation. We found that autophagy is induced at the polychromatic erythroid stage, and that autophagosomes remain abundant until enucleation. This stimulation of autophagy was concomitant with the transcriptional upregulation of many autophagy genes: of note, expression of all ATG8 mammalian paralog family members was stimulated, and increased expression of a subset of ATG4 family members (ATG4A and ATG4D) was also observed. Stable expression of dominant-negative ATG4 cysteine mutants (ATG4B (C74A) ; ATG4D (C144A) ) did not markedly delay or accelerate differentiation of human erythroid cells; however, quantitative EM demonstrated that autophagosomes are assembled less efficiently in ATG4B (C74A) -expressing progenitor cells, and that cells expressing either mutant accumulate enlarged amphisomes that cannot be degraded. The appearance of these hybrid autophagosome/endosome structures correlated with the contraction of the lysosomal compartment, suggesting that the actions of ATG4 family members (particularly ATG4B) are required for the control of autophagosome fusion with late, degradative compartments in differentiating human erythroblasts.
- Autophagy.Autophagy.2013 Jun 1;9(6):881-93. doi: 10.4161/auto.24172. Epub 2013 Mar 18.
- Wholesale depletion of membrane organelles and extrusion of the nucleus are hallmarks of mammalian erythropoiesis. Using quantitative EM and fluorescence imaging we have investigated how autophagy contributes to organelle removal in an ex vivo model of human erythroid differentiation. We found that
- PMID 23508006
Japanese Journal
- A simple method for enrichment of polychromatic erythroblasts from rat bone marrow, and their proliferation and maturation in vitro
- ASANO Hiroyuki,DEGUCHI Yoshihito,KAWAMURA Satoshi,INABA Mutsumi
- Journal of toxicological sciences 36(4), 435-444, 2011-08-01
- … On the other hand, little has been reported on in vitro assays using mature erythroblasts such as polychromatic erythroblasts. … In the present study, we established a convenient method for enrichment of polychromatic erythroblasts from rat bone marrow and confirmed their development in vitro. … To establish a method for the enrichment of polychromatic erythroblasts, bone marrow cells from 3- and 10-week-old rats were separated by discontinuous density gradient centrifugation using Percoll. …
- NAID 10029340237
- 再生不良性貧血に関する研究 特にCeruloplasminを中心とする検討
- 鐘 肇禎
- 昭和医学会雑誌 31(5), 266-277, 1971
- … This results agreed with the animal experimental data which showed the increase of erythropoietic substance in peripheral blood with combined administration of these drugs.<BR>(2) In bone marrow, the increase of nucleated cells and polychromatic erythroblast was remarkable. …
- NAID 130001819983
- Studies on the DNA metabolism of erythroid cell. I. DNA level of erythroblastic nuclei of rabbit bone marrow, observation of normal, blood depleted, and phenylhydrazine anemias, and their recovery by red cell transfusion
- Inoue Masanao
- Acta Medicinae Okayama 24(1), 1970-02
- … <p>For the purpose to reveal the changes in the metabolism of erythroblast in varied specialization stages the author observed the Feulgen DNA level of rabbit erythroblasts by microspectrophotometry. … Twenty-four hours after the massred cell transfusion by which severe anemia has been recovered to the original level within one hour, the pattern of the DNA level of the erythroblast returns to the normal one showing a very low DNA level at the polyandthe orthochromatic stages. …
- NAID 120002311376
Related Links
- erythroblast [ə′rith·rə‚blast] (histology) A nucleated cell occurring in bone marrow as the earliest recognizable cell of the erythrocytic series. Erythroblast intermediate form of development of a red blood cell, or erythrocyte. In ...
- erythroblast [ĕ-rith´ro-blast] a term originally used for any type of nucleated erythrocyte, but now usually limited to one of the nucleated precursors of an erythrocyte, i.e. one of the developmental stages in the erythrocytic series, in ...
★リンクテーブル★
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- 英
- polychromatic erythroblast
- 関
- 赤血球
塩基性赤芽球<-多染性赤芽球->正染性赤芽球
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多染性赤芽球 polychromatic erythroblast
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- 関
- polychromasia