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English Journal
- Molecular definition of distinct cytoskeletal structures involved in complement- and Fc receptor-mediated phagocytosis in macrophages.
- Allen LA1, Aderem A.Author information 1Laboratory of Signal Transduction, Rockefeller University, New York, NY 10021, USA.AbstractIt has long been known from the results of ultrastructural studies that complement- and immunoglobulin G (IgG)-opsonized particles are phagocytosed differently by macrophages (Kaplan. G. 1977. Scand. J. Immunol. 6:797-807). Complement-opsonized particles sink into the cell, whereas IgG-coated particles are engulfed by lamellipodia, which project from the cell surface. The molecular basis for these differences is unknown. We used indirect immunofluorescence and confocal microscopy to examine how cytoskeletal proteins associate with phagosomes containing complement-opsonized zymosan (COZ) particles or IgG beads in phorbol-myristateacetate-treated peritoneal macrophages. During ingestion of COZ, punctate structures rich in F-actin, vinculin, alpha-actinin, paxillin, and phosphotyrosine-containing proteins are distributed over the phagosome surface. These foci are detected beneath bound COZ within 30 s of warming the cells to 37 degrees C, and their formation requires active protein kinase C. By contrast, during Fc receptor-mediated phagocytosis, all proteins examined were uniformly distributed on or near the phagosome surface. Moreover, ingestion of IgG beads was blocked by tyrosine kinase inhibitors, whereas phagocytosis of COZ was not. Thus, the signals required for particle ingestion, and the arrangement of cytoskeletal proteins on the phagosome surface, vary depending upon which phagocytic receptor is engaged. Moreover, complement receptor (CR)-mediated internalization required intact microtubules and was accompanied by the accumulation of vesicles beneath the forming phagosome, suggesting that membrane trafficking plays a key role in CR-mediated phagocytosis.
- The Journal of experimental medicine.J Exp Med.1996 Aug 1;184(2):627-37.
- It has long been known from the results of ultrastructural studies that complement- and immunoglobulin G (IgG)-opsonized particles are phagocytosed differently by macrophages (Kaplan. G. 1977. Scand. J. Immunol. 6:797-807). Complement-opsonized particles sink into the cell, whereas IgG-coated partic
- PMID 8760816
- Entamoeba histolytica depresses chemiluminescence in stimulated human polymorphonuclear leukocytes.
- Al-Mofleh IA1, Al-Tuwaijri AS, Mahmoud AA, Alam M.Author information 1Department of Medicine, College of Medicine, King Saud University, Riyadh, Saudi Arabia.AbstractEffect of Entamoeba histolytica proteinase/toxin (Ehp/t) on the luminol-dependent chemiluminescence (CL) in stimulated human polymorphonuclear leukocytes (PMN) was studied. The role of superoxide (SO) and hydroxyl (OH) anions in the Ehp/t-associated enhancement/inhibition of CL was also studied using specific scavengers and a biological response modifier, muramyldipeptide (MDP). Ehp/t was isolated from axenic trophozoites of the HM-1:IMSS strain of virulent strain of E. histolytica. Proteinase activity was assayed on a synthetic substrate, Z-arg-arg-AFC and cytotoxicity was tested on HeLa cell monolayers. PMN isolated from blood were incubated with Ehp/t prior to stimulation by phorbol myristateacetate (PMA, 2 micrograms/ml), serum-treated zymosan (2.5 mg/ml) and glucan (2 mg/ml). CL was monitored in an LKB (Wallac) Luminometer. Ehp/t was found to depress up to 90% of CL induced by PMA, glucan and zymosan. Such a depression was Ehp/t concentration-dependent. A 150 micrograms/ml concentration of Ehp/t, obtained from a 0.015-1.5 mg/ml concentration range tested at different incubation times and temperatures, was used in most of our experiments. Incubation time and temperature optima were 15 min and 37 degrees C, respectively. Ehp/t partially inhibited the CL associated with SO and OH. MDP, in the presence of Ehp/t, enhanced CL response in human PMN to about 67% with reference to normal CL without inhibitor. PMN were confirmed to play a vital role in amebic tissue invasion mechanisms.
- International journal of immunopharmacology.Int J Immunopharmacol.1989;11(5):529-36.
- Effect of Entamoeba histolytica proteinase/toxin (Ehp/t) on the luminol-dependent chemiluminescence (CL) in stimulated human polymorphonuclear leukocytes (PMN) was studied. The role of superoxide (SO) and hydroxyl (OH) anions in the Ehp/t-associated enhancement/inhibition of CL was also studied usin
- PMID 2553622
- Activators of protein kinase C and phenylephrine depolarize the astrocyte membrane by reducing the K+ permeability.
- Akerman KE1, Enkvist MO, Holopainen I.Author information 1Abo Akademi, Department of Biochemistry and Pharmacy, Finland.AbstractThe membrane potential of astrocytes has been measured by monitoring the absorbance of a cyanine dye DiS-C2-(5). Ba2+, the phorbol ester 12-tetradecanoylphorbol myristateacetate (TPA) and the diglyceride, dioctanoylglycerol (DiC8) depolarize the membrane. Valinomycin which makes the membrane potential dependent on the K+ electrochemical potential evokes a hyperpolarization when added subsequently. The alpha-adrenergic receptor agonist phenylephrine was blocked by Ba2+, TPA, DiC8 and valinomycin. The results suggest that a protein kinase C-mediated reduction in the K+ permeability is responsible for the depolarizing effect of TPA, DiC8 and phenylephrine.
- Neuroscience letters.Neurosci Lett.1988 Oct 17;92(3):265-9.
- The membrane potential of astrocytes has been measured by monitoring the absorbance of a cyanine dye DiS-C2-(5). Ba2+, the phorbol ester 12-tetradecanoylphorbol myristateacetate (TPA) and the diglyceride, dioctanoylglycerol (DiC8) depolarize the membrane. Valinomycin which makes the membrane potenti
- PMID 3200485
Japanese Journal
- （１→３）‐β‐Ｄ‐グルカンと抗真菌薬のヒト多形核白血球の化学発光におよぼす影響
- 加藤 淳子
- 日本化学療法学会雑誌 47(3), 152-160, 1999
- … LPSと可溶性β-グルカンの好中球CLに対するpriming効果を比較するために, 好中球をLPSや3種類の可溶性β-グルカンで37℃ で60分間前処理した後で, phorbol myristateacetate (PMA) で刺激して20分間のCLを測定した。 …
- NAID 130004297911
- Co-localization of Urokinase and its Receptor on Established Human Umbilical Vein Endothelial Cell.
- Ueshima Shigeru,Matsumoto Hiroshi,Izaki Seiichi,Mitsui Youji,Fukao Hideharu,Okada Kiyotaka,Matsuo Osamu
- Cell Structure and Function 24(2), 71-78, 1999
- … The treatment of endothelial cells with phorbol-myristateacetate (PMA) upregulated the expression of u-PA and u-PAR antigens. …
- NAID 130004137289
- 菅野 和久,徳永 賢治,越智 正昭,宍野 宏治,村瀬 光春,佐伯 修一,武内 望,篠原 力雄,石黒 伊三雄
- 臨床化学 22(3), 168-172, 1993
- … 糖尿病患者における合併症や易感染性の原因とその機序を解明する目的でphorbo-myristateacetate刺激時 (PMA) およびオプソニン化チモーザン刺激時 (OZ) の好中球活性酸素産生能を測定し, さらに血中糖化タンパク (HbA1c, フルクトサミン) 濃度との関連性を調べた。 …
- NAID 130003357731
Related Links
- What Is Phorbol Myristate Acetate?. Phorbol Myristate Acetate is a biologically active compound that is derived from a plant. Studies have shown that this compound may be effective in treating a variety of medical ...
- Phorbol 12-myristate 13-acetate (PMA), also known as 12-O-tetradecanoylphorbol 13-acetate (TPA) is a specific activator of Protein Kinase C (PKC) and hence of NF-kB. PMA is the most commonly used phorbol ester. It is active at ...
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