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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2012/09/29 23:34:37」(JST)

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English Journal

Tuning the activities and structures of enzymes bound to graphene oxide with a protein glue.

Pattammattel A, Puglia M, Chakraborty S, Deshapriya IK, Dutta PK, Kumar CV.Author information Department of Chemistry, University of Connecticut , Department of Molecular and Cell Biology, and the Institute of Material Science, 55 North Eagleville Road, Unit 3060, Storrs, Connecticut 06269-3060, United States.AbstractGraphene oxide (GO) is being investigated extensively for enzyme and protein binding, but many enzymes bound to GO denature considerably and lose most of their activities. A simple, novel, and efficient approach is described here for improving the structures and activities of enzymes bound to GO such that bound enzymes are nearly as active as those of the corresponding unbound enzymes. Our strategy is to preadsorb highly cationized bovine serum albumin (cBSA) to passivate GO, and cBSA/GO (bGO) served as an excellent platform for enzyme binding. The binding of met-hemoglobin, glucose oxidase, horseradish peroxidase, BSA, catalase, lysozyme, and cytochrome c indicated improved binding, structure retention, and activities. Nearly 100% of native-like structures of all the seven proteins/enzymes were noted at near monolayer formation of cBSA on GO (400% w/w), and all bound enzymes indicated 100% retention of their activities. A facile, benign, simple, and general method has been developed for the biofunctionalization of GO, and this approach of coating with suitable protein glues expands the utility of GO as an advanced biophilic nanomaterial for applications in catalysis, sensing, and biomedicine.
Langmuir : the ACS journal of surfaces and colloids.Langmuir.2013 Dec 17;29(50):15643-54. doi: 10.1021/la404051c. Epub 2013 Dec 5.
Graphene oxide (GO) is being investigated extensively for enzyme and protein binding, but many enzymes bound to GO denature considerably and lose most of their activities. A simple, novel, and efficient approach is described here for improving the structures and activities of enzymes bound to GO suc
PMID 24274382

Labeling of human hepatocellular carcinoma cells by hexamethylene diamine modified fluorescent carbon dots.

Dong W, Dong Y, Wang Y, Zhou S, Ge X, Sui L, Wang J.Author information Department of Chemistry, Shenyang Medical College, Shenyang 110034, China. Electronic address: dongwei5873@126.com.AbstractFluorescent carbon dots (CDs) were synthesized by a solvothermal method with glucose as carbon source and surface-modified with 1,6-hexamethylene diamine. In this hybrid CDs, the modification played important role for improving the fluorescent performance by introducing nitrogenous compound to passivate CD's surface, making the CDs emit strong fluorescence. The as-prepared CDs were linked with mouse anti-human Alpha fetoprotein (AFP) antibody and goat anti-mouse immunoglobulin (IgG) to directly and indirectly label fixed human hepatocellular carcinoma cells, respectively. The cytotoxicity of these CDs were also tested using the human hepatocellular carcinoma cells. No apparent cytotoxicity was observed, which suggested the potential application of the as-prepared CDs in bioimaging.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.Spectrochim Acta A Mol Biomol Spectrosc.2013 Dec;116:209-13. doi: 10.1016/j.saa.2013.07.012. Epub 2013 Jul 16.
Fluorescent carbon dots (CDs) were synthesized by a solvothermal method with glucose as carbon source and surface-modified with 1,6-hexamethylene diamine. In this hybrid CDs, the modification played important role for improving the fluorescent performance by introducing nitrogenous compound to passi
PMID 23933844

Modification of hybrid active bilayer for enhanced efficiency and stability in planar heterojunction colloidal quantum dot photovoltaics.

Heo SJ, Yoon S, Oh SH, Kim HJ.AbstractSolution-processed planar heterojunction colloidal quantum dot photovoltaics with a hybrid active bilayer is demonstrated. A power conversion efficiency of 1.24% under simulated air mass 1.5 illumination conditions is reported. This was achieved through solid-state treatment with cetyltrimethylammonium bromide of PbS colloidal quantum dot solid films. That treatment was used to passivate Br atomic ligands as well as to engineer the interface within the hybrid active bilayer.
Nanoscale research letters.Nanoscale Res Lett.2013 Nov 20;8(1):488. [Epub ahead of print]
Solution-processed planar heterojunction colloidal quantum dot photovoltaics with a hybrid active bilayer is demonstrated. A power conversion efficiency of 1.24% under simulated air mass 1.5 illumination conditions is reported. This was achieved through solid-state treatment with cetyltrimethylammon
PMID 24252664