English Journal

Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B.

Montellier E, Boussouar F, Rousseaux S, Zhang K, Buchou T, Fenaille F, Shiota H, Debernardi A, Héry P, Curtet S, Jamshidikia M, Barral S, Holota H, Bergon A, Lopez F, Guardiola P, Pernet K, Imbert J, Petosa C, Tan M, Zhao Y, Gérard M, Khochbin S.SourceU823, Institut National de la Santé et de la Recherche Médicale INSERM, Institut Albert Bonniot, Université Joseph Fourier, Grenoble F-38700 France.
Genes & development.Genes Dev.2013 Aug 1;27(15):1680-92. doi: 10.1101/gad.220095.113. Epub 2013 Jul 24.
The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental to the life cycle, yet the underlying molecular details remain obscure. Here we show that an essential step is the genome-wide incorporation of TH2B, a histone H2B variant of hitherto unknown function. Using mou
PMID 23884607

Sperm head vacuoles--light microscopic and ultrastructural observations: a case report.

Zhang S, Wang N, He B, Cheng J, Xi S, Wang SM, Gao Y, Wang J.SourceNational Research Institute for Family Planning, Beijing, People's Republic of China.
Ultrastructural pathology.Ultrastruct Pathol.2012 May;36(3):185-8. doi: 10.3109/01913123.2012.658988.
BACKGROUND: Sperm head vacuoles are easily detectable in human spermatozoa under the electron microscope. A sperm head vacuole is considered abnormal when it exceeds 20% of the head's cross-sectional area. The authors report a rare case of primary spermatozoa deformity with 100% vacuolated head and
PMID 22559046

[Chromatin folding in human spermatozoa. I. Dynamics of chromatin remodelling in differentiating human spermatids].

Arifulin EA, Bragina EE, Zamiatnina VA, Volkova EG, Sheval' EV, Golyshev SA, Kintsurashvili LN, Kir'ianov GI, Prusov AN, Poliakov VIu.AbstractChanges in chromatin structure at different stages of differentiation of human spermatids were studied. It was shown that, in nuclei of early spermatids, chromatin is loosely packed and its structural element is an 8-nm fiber. This "elementary" fiber is predominant at the initial stages of differentiation; in the course of maturation, it is replaced by globular elements approximately 60 nm in diameter. In intermediate spermatids, these globules start to condense into fibrillar aggregates and reduce their diameter to 30-40 nm. At all stages of spermatid maturation, except the final stages, these globules are convergence centers for elementary fibers. This remodelling process is vectored and directed from the apical (acrosomal) to the basal pole of the nucleus. In mature spermatids, the elementary 8-nm fibers are almost absent and the major components are 40-nm fibrillar aggregates. The nuclei of mature spermatids are structurally identical with the nuclei of spermatozoa with the so-called "immature chromatin," which are commonly found in a low proportion in sperm samples from healthy donors and may prevail over the normal cells in spermiogenetic disorders. The cause of this differentiation blockade remains unknown. Possibly, the formation of intermolecular bonds between protamines, which are required for the final stages of chromatin condensation, is blocked in a part of spermatids. The results of this study are discussed in comparison with the known models of nucleoprotamine chromatin organization in human spermatozoa.
Ontogenez.Ontogenez.2012 Mar-Apr;43(2):143-53.
Changes in chromatin structure at different stages of differentiation of human spermatids were studied. It was shown that, in nuclei of early spermatids, chromatin is loosely packed and its structural element is an 8-nm fiber. This "elementary" fiber is predominant at the initial stages of different
PMID 22650080

Japanese Journal

Nucleoprotamine diet derived from salmon soft roe protects mouse hippocampal neurons from delayed cell death after transient forebrain ischemia

MATSUNAGA Masaji,OHTAKI Hirokazu,TAKAKI Atsushi,IWAI Yumiko,YIN Li,MIZUGUCHI Hiroko,MIYAKE Takeo,USUMI Koji,SHIODA Seiji
Neuroscience research : the official journal of the Japan Neuroscience Society 47(3), 269-276, 2003-11-01
NAID 10016208698

デオキシペントース核蛋白の抽出・精製およびその化学的性状の研究

鈴木〓之
東京大學理工學研究所報告 11(14), 177-217, 1957-12-24
… For the preparation of nucleoprotamine, sperm heads were collected and washed by centrifugation and extracted with strong saline. …
NAID 110001038629

超遠心機によるデオキシペントース核酸および核蛋白の研究

川出由己
東京大學理工學研究所報告 10(12), 149-196, 1957-02-25
… Interaction between DNA and basic protein in strong saline at neutral pH was studied in cases of nucleohistone from calf thymus (TNH), nucleoprotamine from sperm of rainbow trout and artificial mixture of purified preparations of DNA and histone. … In sharp contrast to TNH, nucleoprotamine seemed to be fully dissociated into DNA and protamine throughout the range of sodium chloride concentration studied (0.83〜2.9M). …
NAID 110001038615