English Journal

Characterization of an extremely thermostable but cold-adaptive β-galactosidase from the hyperthermophilic archaeon Pyrococcus furiosus for use as a recombinant aggregation for batch lactose degradation at high temperature.

Dong Q1, Yan X2, Zheng M3, Yang Z3.
Journal of bioscience and bioengineering.J Biosci Bioeng.2014 Jun;117(6):706-10. doi: 10.1016/j.jbiosc.2013.12.002. Epub 2014 Jan 24.
β-Galactosidase (lactase), which catalyzes the hydrolysis of lactose into glucose and galactose, is one of the most important enzymes used in dairy processing. In this study, a gene that encoded an extremely thermostable β-galactosidase from Pyrococcus furiosus (Pflactase) was cloned and expressed
PMID 24462527

Biochemical and kinetic characterization of the multifunctional β-glucosidase/β-xylosidase/α-arabinosidase, Bgxa1.

Gruninger RJ1, Gong X, Forster RJ, McAllister TA.
Applied microbiology and biotechnology.Appl Microbiol Biotechnol.2014 Apr;98(7):3003-12. doi: 10.1007/s00253-013-5191-4. Epub 2013 Aug 31.
Functional screening of a metagenomic library constructed with DNA extracted from the rumen contents of a grass/hay-fed dairy cow identified a protein, β-glucosidase/β-xylosidase/α-arabinosidase gene (Bgxa1), with high levels of β-glucosidase activity. Purified Bgxa1 was highly active against p-
PMID 23995226

High level production of β-galactosidase exhibiting excellent milk-lactose degradation ability from Aspergillus oryzae by codon and fermentation optimization.

Zhao Q1, Liu F, Hou Z, Yuan C, Zhu X.
Applied biochemistry and biotechnology.Appl Biochem Biotechnol.2014 Mar;172(6):2787-99. doi: 10.1007/s12010-013-0684-2. Epub 2014 Jan 18.
A β-galactosidase gene from Aspergillus oryzae was engineered utilizing codon usage optimization to be constitutively and highly expressed in the Pichia pastoris SMD1168H strain in a high-cell-density fermentation. After fermentation for 96 h in a 50-L fermentor using glucose and glycerol as combin
PMID 24435763

Related Pictures

Experiment Three. Does the amount of In nature, where is lactase found? Ortho-Nitrophenyl Galactoside Test on Experiment Two. How does the hydrolysis of By substituting nitrophenylgalactoside (NP is to use o-nitrophenylgalactoside nitrophenyl galactoside as substrate gus