English Journal

[Expression of fused luxAB gene of bacterial luciferase in liver carcinoma cells].

Mao C1, Yang S, Zhao M.
Zhonghua zhong liu za zhi [Chinese journal of oncology].Zhonghua Zhong Liu Za Zhi.2001 Sep;23(5):359-62.
OBJECTIVE: This work was done to look into the expression of fused luxAB gene of bacterial luciferase as a reporter gene in liver carcinoma cells.METHODS: The mammalian expression vector pcDNA3-luxAB gene, constructed by the fusion of luxA and luxB genes, were amplified in the polymerase chain react
PMID 11810760

Identification and mutagenesis by allelic exchange of choE, encoding a cholesterol oxidase from the intracellular pathogen Rhodococcus equi.

Navas J1, González-Zorn B, Ladrón N, Garrido P, Vázquez-Boland JA.
Journal of bacteriology.J Bacteriol.2001 Aug;183(16):4796-805.
The virulence mechanisms of the facultative intracellular parasite Rhodococcus equi remain largely unknown. Among the candidate virulence factors of this pathogenic actinomycete is a secreted cholesterol oxidase, a putative membrane-damaging toxin. We identified and characterized the gene encoding t
PMID 11466283

González-Zorn B1, Domínguez-Bernal G, Suárez M, Ripio MT, Vega Y, Novella S, Rodríguez A, Chico I, Tierrez A, Vázquez-Boland JA.
International journal of medical microbiology : IJMM.Int J Med Microbiol.2000 Oct;290(4-5):369-74.
We describe here the fourth listerial membrane-damaging virulence factor, a sphingomyelinase C (SMase) that is produced specifically by the ruminant pathogen Listeria ivanovii. Its coding gene, smcL, is a monocistron expressed independently of PrfA. The smcL product, SmcL, is highly similar to the s
PMID 11111913