English Journal

Frequent carriage of resistance mechanisms to β-lactams and biofilm formation in Haemophilus influenzae causing treatment failure and recurrent otitis media in young children.

García-Cobos S1, Moscoso M2, Pumarola F3, Arroyo M4, Lara N4, Pérez-Vázquez M4, Aracil B4, Oteo J4, García E2, Campos J5.
The Journal of antimicrobial chemotherapy.J Antimicrob Chemother.2014 Sep;69(9):2394-9. doi: 10.1093/jac/dku158. Epub 2014 Jun 2.
OBJECTIVES: Non-typeable Haemophilus influenzae are a major cause of acute otitis media (AOM), including chronic and recurrent otitis in young children. The objective of this study was to determine whether non-typeable H. influenzae isolates causing these infections produce biofilms and carry resist
PMID 24891432

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay is a rapid, cheap, screening test for the in vitro anti-tuberculous activity of chalcones.

Moodley S1, Koorbanally NA2, Moodley T2, Ramjugernath D3, Pillay M4.
Journal of microbiological methods.J Microbiol Methods.2014 Sep;104:72-8. doi: 10.1016/j.mimet.2014.06.014. Epub 2014 Jun 28.
Rapid and reliable drug susceptibility testing facilitates replenishment of the TB drug pipeline in the fight against drug resistant Mycobacterium tuberculosis. This study compared the performance of the MTT and MABA assays on the anti-tuberculous activity of a set of chalcones. Twenty seven chalcon
PMID 24978593

Optimisation of the Microplate Resazurin Assay for Screening and Bioassay-guided Fractionation of Phytochemical Extracts against Mycobacterium tuberculosis.

O'Neill TE1, Li H, Colquhoun CD, Johnson JA, Webster D, Gray CA.
Phytochemical analysis : PCA.Phytochem Anal.2014 Sep;25(5):461-7. doi: 10.1002/pca.2516. Epub 2014 Apr 14.
INTRODUCTION: Because of increased resistance to current drugs, there is an urgent need to discover new anti-mycobacterial compounds for the development of novel anti-tuberculosis drugs. The microplate resazurin assay (MRA) is commonly used to evaluate natural products and synthetic compounds for an
PMID 24733665

Japanese Journal

Novel synergistic approach to exploit the bactericidal efficacy of commercial disinfectants on the biofilms of Salmonella enterica serovar Typhimurium(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

Singla Richu,Goel Honey,Ganguli Abhijit
Journal of bioscience and bioengineering 118(1), 34-40, 2014-07
… Five- and six-fold reduction in biofilm formation was observed in microtitre plates after 20 h and 40 h. …
NAID 110009838766

Resazurin microtitre plate method for screening of anti-microbial resistance from Thai medical plants

Membrane eicosapentaenoic acid is involved in the hydrophobicity of bacterial cells and affects the entry of hydrophilic and hydrophobic compounds

Nishida Takanori,Hori Ryuji,Morita Naoki,Okuyama Hidetoshi
FEMS Microbiology Letters 306(2), 91-96, 2010-05
… Eicosapentaenoic acid (EPA)-producing Shewanella marinintestina IK-1 (IK-1) and its EPA-deficient mutant IK-1Δ8 (IK-1Δ8) were grown on microtitre plates at 20 °C in a nutrient medium that contained various types of growth inhibitors. …
NAID 120003005547