microelectrophoresis

• 微小電気泳動、微小電気泳動法

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出典(authority):フリー百科事典『ウィキペディア（Wikipedia）』「2016/03/02 11:36:17」(JST)

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Microelectrophoresis is a method of studying electrophoresis of various dispersed particles using optical microscopy. This method provides image of moving particles, which is its unique advantage. e.g. observation of RBCs, neutrophiles and bacteria. This type of electrophoresis is carried out in a closed medium with critical observations by focusing and adjusting the lens of microscope.

Complexity of this method is associated with electro-osmosis generated by electric field influence on the double layers of the sample cell walls. In the usually used closed cells this creates Poiseuille type back flow, leading to parabolic velocity profile. There are two stationary layers, where fluid does not move. Position of these stationary layer regarding cell walls depends on the cell geometry. It is possible to focus microscope on this stationary layers and observe particles motion not affected with electro-osmosis.

The second complexity comes from necessity of diluting sample, if it was initially concentrated. Concentration must be sufficiently low for observing individual particles.

Measurement in the low polar fluids present additional problems. These systems have low electrical conductivity and low dielectric permittivity. Electrophoretic mobility is low and this requires high electric field, by factor 10 and higher.

Details of this method are presented in IUPAC Technical Report prepared by a group of most known world experts on the electrokinetic phenomena.

References

English Journal

• Core-shell polydopamine magnetic nanoparticles as sorbent in micro-dispersive solid-phase extraction for the determination of estrogenic compounds in water samples prior to high-performance liquid chromatography-mass spectrometry analysis.

• Socas-Rodríguez B1, Hernández-Borges J2, Salazar P3, Martín M4, Rodríguez-Delgado MÁ5.
• Journal of chromatography. A.J Chromatogr A.2015 Jun 5;1397:1-10. doi: 10.1016/j.chroma.2015.04.010. Epub 2015 Apr 13.
• In this work, core-shell Fe3O4@poly(dopamine) magnetic nanoparticles (m-NPs) were prepared and characterized in our laboratory and applied as sorbents for the magnetic-micro solid phase extraction (m-μSPE) of twelve estrogenic compounds of interest (i.e. 17α-estradiol, 17β-estradiol, estrone, hex
• PMID 25917309

• Adsorption of cytochrome c on montmorillonite nanoplates: protein concentration dependence.

• Hristova SH1, Zhivkov AM2.
• Journal of colloid and interface science.J Colloid Interface Sci.2015 May 15;446:252-62. doi: 10.1016/j.jcis.2015.01.039. Epub 2015 Jan 24.
• Cytochrome c [cytC] is a mitochondrial hemoprotein functioning as electron carrier in the respiratory chain of the biological cells. Being adsorbed on colloid particles cytC can be introduced in the cells by phagocytoses. In the present work we study the adsorption of cytC on montmorillonite (MM) pa
• PMID 25678159

• Impact of polyacrylamide with different contents of carboxyl groups on the chromium (III) oxide adsorption properties in aqueous solution.

• Wiśniewska M1, Chibowski S2, Urban T2.
• Journal of hazardous materials.J Hazard Mater.2015 Feb 11;283:815-23. doi: 10.1016/j.jhazmat.2014.10.037. Epub 2014 Nov 1.
• The main goal of experiments was determination of solution pH and contents of anionic groups in polyacrylamide (PAM) macromolecules on the stability mechanism of chromium (III) oxide suspension. The spectrophotometry, potentiometric titration, microelectrophoresis, viscosimetry and turbidimetry were
• PMID 25464324

Japanese Journal

• ガラスビーズ及び鹿沼土充填カラムにおけるコロイド粒子の捕捉に関する界面動電的測定に基づく考察

• 農業土木学会論文集 2006(245), 729-735, 2006
• NAID 130004348812

• Synergy between the CoMoS Phase and Supported or Unsupported Cobalt Sulfide. Existence of a Remote Control Effect.

• Bulletin of the Chemical Society of Japan 68(1), 107-119, 1995
• NAID 130004148964

• ヒスタミン遊離研究の展開

• 藥學雜誌 114(3), 147-159, 1994-03-25
• NAID 110003649476

Related Links

• Microelectrophoresis | Define Microelectrophoresis at ...

Microelectrophoresis definition, any of several techniques for observing, by means of a microscope or an ultramicroscope, the electrophoresis of minute surface particles. See more. Dictionary.com Word of the Day Translate Games ...

• Microelectrophoresis | Definition of microelectrophoresis by ...

Definition of MICROELECTROPHORESIS: electrophoresis in which the movement of single particles is observed in a microscope; also: electrophoresis in which micromethods are used — mi · cro · elec · tro · pho · ret · ic \-ˈ re-tik\ adjective

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